Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,273,231 A→T 8.0% Y216N (TAC→AAC)  glcC ← transcriptional regulator GlcC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,273,2310AT 8.0% 145.9 / 3.2 50Y216N (TAC→AAC) glcCtranscriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base T (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

CTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCT  >  NC_002947/4273083‑4273371
                                                                                                                                                    |                                                                                                                                            
cTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTa                                                                                                                                              <  1:101435/149‑1 (MQ=255)
   cACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACa                                                                                                                                            <  7:120561/148‑1 (MQ=255)
     cccccAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAAcc                                                                                                                                         >  6:156904/1‑149 (MQ=255)
         cAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTTGTACCgcgc                                                                                                                                     <  8:40354/149‑1 (MQ=255)
          aGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTTGTACCGCGCa                                                                                                                                    <  2:66059/149‑1 (MQ=255)
           gCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAt                                                                                                                                   >  8:262604/1‑149 (MQ=255)
              atccatACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgct                                                                                                                                >  2:250485/1‑149 (MQ=255)
                 catACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgctgc                                                                                                                              <  3:53696/148‑1 (MQ=255)
                   taggCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCAGGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGa                                                                                                                           <  2:306381/146‑1 (MQ=255)
                     cGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATc                                                                                                                         <  7:48199/149‑1 (MQ=255)
                         tGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATc                                                                                                                     >  3:253676/1‑149 (MQ=255)
                                 cTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGtt                                                                                                              >  6:198877/1‑148 (MQ=255)
                                     ccAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTgc                                                                                                         >  4:145283/1‑149 (MQ=255)
                                      cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgct                                                                                                                                >  7:273239/1‑125 (MQ=255)
                                      cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgct                                                                                                                                <  8:273239/125‑1 (MQ=255)
                                      cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTgcg                                                                                                        >  8:216593/1‑149 (MQ=255)
                                             tgctCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTa                                                                                                                                              >  4:336329/1‑104 (MQ=255)
                                             tgctCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTa                                                                                                                                              <  3:336329/104‑1 (MQ=255)
                                               ctctTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCgg                                                                                                <  2:342402/148‑1 (MQ=255)
                                               ctctTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCgg                                                                                                <  5:198877/148‑1 (MQ=255)
                                                tctTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCa                                                                                              >  8:302025/1‑149 (MQ=255)
                                                    ccTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTa                                                                                           >  8:338313/1‑148 (MQ=255)
                                                        cTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgt                                                                                      >  8:104010/1‑149 (MQ=255)
                                                           gATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                    <  5:367783/148‑1 (MQ=255)
                                                           gATCTCCCGCAAACTGTCGCGTATGCCGATGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCACGCATGCTGCCGATCAATCTGGCGTTTCTGCGATGGCCGCCAGAACAAgttgt                                                                                   >  3:239733/1‑149 (MQ=255)
                                                                 ccGCAAACTGTCGCGTAGGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                    <  2:319934/142‑1 (MQ=255)
                                                                 cagCATACTGTCGGGGATGCCGTTGATATGCTCACGCGCCGAACGCTGCGCCTGCTCGGGCAGTTTTTCCATGACTTCTCGGTACAACCGCGCATGCTGCCGATCAATCTGTCGTTTCTGCGCTGGCCGGCAGTACAAgttgtt                                                                                  <  8:257387/142‑1 (MQ=255)
                                                                        cTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                      <  7:216593/149‑1 (MQ=255)
                                                                         tGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                      >  4:352284/1‑148 (MQ=255)
                                                                         tGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                      >  2:39924/1‑148 (MQ=255)
                                                                             gcgTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACAcc                                                                  <  4:253676/148‑1 (MQ=255)
                                                                              cgTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATGAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGAGCGACGCAAACACCGt                                                                <  6:370634/149‑1 (MQ=255)
                                                                                tATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGc                                                              <  7:302025/149‑1 (MQ=255)
                                                                                  tGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTc                                                            <  5:156904/149‑1 (MQ=255)
                                                                                    ccGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                    <  2:271222/123‑1 (MQ=255)
                                                                                    ccGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                    >  1:271222/1‑123 (MQ=255)
                                                                                      gTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACg                                                        >  2:268403/1‑149 (MQ=255)
                                                                                                tCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACt                                              >  4:102754/1‑149 (MQ=255)
                                                                                                  aCGCGCCGCCAGCTGCGCCTTCTCGTGCAGTTTTTCCATGACTTCTCGGTACAAACTCGCATTCTGCCGATCAATCTGGCGTTTCTGCTCTGGCCGGGAGTACAAGTTGTTTACCGACGCAAAGACCGTGCTCAACGTCAGGTCACt                                              <  7:13573/147‑1 (MQ=255)
                                                                                                      gccgccCGCTGCGCCTGCTCGGGGAGTTGTTCCATGTCTGGGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCATCGTCAGGTCACTGAGCGa                                        <  4:239733/149‑1 (MQ=255)
                                                                                                            cgcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCa                                                                                              >  7:330624/1‑89 (MQ=255)
                                                                                                            cgcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCa                                                                                              <  8:330624/89‑1 (MQ=255)
                                                                                                                 cgcCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGt                               <  1:250485/147‑1 (MQ=255)
                                                                                                                       cTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAACCACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCAc                         <  1:268403/147‑1 (MQ=255)
                                                                                                                       cTCGGCCAGTTCTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTCGCGTTTCTGCGCTGCCCGGCAGTACAAGTTGTTGA‑CGACGCAAACACCGTGCTCAACCTCAGGTCACTGAGCGAATGCAGCGTATGCACCAg                      >  2:28524/1‑149 (MQ=255)
                                                                                                                                     ccATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGc         >  8:312318/1‑149 (MQ=255)
                                                                                                                                      cATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCa                       <  5:175616/134‑1 (MQ=255)
                                                                                                                                      cATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCa                       >  6:175616/1‑134 (MQ=255)
                                                                                                                                      cATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGc         >  1:204803/1‑148 (MQ=255)
                                                                                                                                       cagcatGCGCGGTTGTACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGa       >  8:341954/6‑149 (MQ=255)
                                                                                                                                            tGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCt  >  2:53702/1‑149 (MQ=255)
                                                                                                                                            tGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCt  >  3:12950/1‑149 (MQ=255)
                                                                                                                                             gcgcGGTTGTACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCt  >  4:167829/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCT  >  NC_002947/4273083‑4273371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑