Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,273,232 C→G 8.0% L215F (TTG→TTC) ‡ glcC ← transcriptional regulator GlcC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,273,2320CG 8.0% 155.2 / 4.5 50L215F (TTG→TTC) ‡glcCtranscriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base G (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

CACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATG  >  NC_002947/4273086‑4273379
                                                                                                                                                  |                                                                                                                                                   
cACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACa                                                                                                                                                    <  7:120561/148‑1 (MQ=255)
  cccccAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAAcc                                                                                                                                                 >  6:156904/1‑149 (MQ=255)
      cAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTTGTACCgcgc                                                                                                                                             <  8:40354/149‑1 (MQ=255)
       aGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTTGTACCGCGCa                                                                                                                                            <  2:66059/149‑1 (MQ=255)
        gCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAt                                                                                                                                           >  8:262604/1‑149 (MQ=255)
           atccatACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgct                                                                                                                                        >  2:250485/1‑149 (MQ=255)
              catACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgctgc                                                                                                                                      <  3:53696/148‑1 (MQ=255)
                taggCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCAGGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGa                                                                                                                                   <  2:306381/146‑1 (MQ=255)
                  cGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATc                                                                                                                                 <  7:48199/149‑1 (MQ=255)
                      tGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATc                                                                                                                             >  3:253676/1‑149 (MQ=255)
                              cTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGtt                                                                                                                      >  6:198877/1‑148 (MQ=255)
                                  ccAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTgc                                                                                                                 >  4:145283/1‑149 (MQ=255)
                                   cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgct                                                                                                                                        <  8:273239/125‑1 (MQ=255)
                                   cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCAtgct                                                                                                                                        >  7:273239/1‑125 (MQ=255)
                                   cAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTgcg                                                                                                                >  8:216593/1‑149 (MQ=255)
                                            ctctTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCgg                                                                                                        <  2:342402/148‑1 (MQ=255)
                                            ctctTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCgg                                                                                                        <  5:198877/148‑1 (MQ=255)
                                             tctTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCa                                                                                                      >  8:302025/1‑149 (MQ=255)
                                                 ccTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTa                                                                                                   >  8:338313/1‑148 (MQ=255)
                                                     cTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgt                                                                                              >  8:104010/1‑149 (MQ=255)
                                                        gATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                            <  5:367783/148‑1 (MQ=255)
                                                        gATCTCCCGCAAACTGTCGCGTATGCCGATGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCACGCATGCTGCCGATCAATCTGGCGTTTCTGCGATGGCCGCCAGAACAAgttgt                                                                                           >  3:239733/1‑149 (MQ=255)
                                                              ccGCAAACTGTCGCGTAGGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                            <  2:319934/142‑1 (MQ=255)
                                                              cagCATACTGTCGGGGATGCCGTTGATATGCTCACGCGCCGAACGCTGCGCCTGCTCGGGCAGTTTTTCCATGACTTCTCGGTACAACCGCGCATGCTGCCGATCAATCTGTCGTTTCTGCGCTGGCCGGCAGTACAAgttgtt                                                                                          <  8:257387/142‑1 (MQ=255)
                                                                     cTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                              <  7:216593/149‑1 (MQ=255)
                                                                      tGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                              >  4:352284/1‑148 (MQ=255)
                                                                      tGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCaaa                                                                              >  2:39924/1‑148 (MQ=255)
                                                                          gcgTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACAcc                                                                          <  4:253676/148‑1 (MQ=255)
                                                                           cgTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATGAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGAGCGACGCAAACACCGt                                                                        <  6:370634/149‑1 (MQ=255)
                                                                             tATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGc                                                                      <  7:302025/149‑1 (MQ=255)
                                                                               tGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTc                                                                    <  5:156904/149‑1 (MQ=255)
                                                                                 ccGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                            >  1:271222/1‑123 (MQ=255)
                                                                                 ccGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAgttg                                                                                            <  2:271222/123‑1 (MQ=255)
                                                                                   gTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACg                                                                >  2:268403/1‑149 (MQ=255)
                                                                                             tCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACt                                                      >  4:102754/1‑149 (MQ=255)
                                                                                               aCGCGCCGCCAGCTGCGCCTTCTCGTGCAGTTTTTCCATGACTTCTCGGTACAAACTCGCATTCTGCCGATCAATCTGGCGTTTCTGCTCTGGCCGGGAGTACAAGTTGTTTACCGACGCAAAGACCGTGCTCAACGTCAGGTCACt                                                      <  7:13573/147‑1 (MQ=255)
                                                                                                   gccgccCGCTGCGCCTGCTCGGGGAGTTGTTCCATGTCTGGGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCATCGTCAGGTCACTGAGCGa                                                <  4:239733/149‑1 (MQ=255)
                                                                                                         cgcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCa                                                                                                      >  7:330624/1‑89 (MQ=255)
                                                                                                         cgcTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCa                                                                                                      <  8:330624/89‑1 (MQ=255)
                                                                                                              cgcCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGt                                       <  1:250485/147‑1 (MQ=255)
                                                                                                                    cTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAACCACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCAc                                 <  1:268403/147‑1 (MQ=255)
                                                                                                                    cTCGGCCAGTTCTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTCGCGTTTCTGCGCTGCCCGGCAGTACAAGTTGTTGA‑CGACGCAAACACCGTGCTCAACCTCAGGTCACTGAGCGAATGCAGCGTATGCACCAg                              >  2:28524/1‑149 (MQ=255)
                                                                                                                                  ccATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGc                 >  8:312318/1‑149 (MQ=255)
                                                                                                                                   cATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCa                               >  6:175616/1‑134 (MQ=255)
                                                                                                                                   cATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCa                               <  5:175616/134‑1 (MQ=255)
                                                                                                                                   cATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGc                 >  1:204803/1‑148 (MQ=255)
                                                                                                                                    cagcatGCGCGGTTGTACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGa               >  8:341954/6‑149 (MQ=255)
                                                                                                                                         tGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCt          >  3:12950/1‑149 (MQ=255)
                                                                                                                                         tGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCt          >  2:53702/1‑149 (MQ=255)
                                                                                                                                          gcgcGGTTGTACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCt          >  4:167829/1‑148 (MQ=255)
                                                                                                                                                  cAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATg  <  7:338313/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGGGCAGTTGTTCCATGACTGCGCGGTACAACCGCGCATGCTGCCGATCAATCTGGCGTTTCTGCGCTGGCCGGCAGTACAAGTTGTTGACCGACGCAAACACCGTGCTCAACGTCAGGTCACTGAGCGATTGCAGGGTATGCACCAGCACTGGGTTATGCGACGCCTCACTGATG  >  NC_002947/4273086‑4273379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑