Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,279,813 T→G 6.9% L338R (CTG→CGG)  PP_3750 → GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,279,8130TG 6.9% 181.2 / 3.8 58L338R (CTG→CGG) PP_3750GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (29/25);  new base G (2/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGACTGTGAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCG  >  NC_002947/4279665‑4279957
                                                                                                                                                    |                                                                                                                                                
tacgactGTGAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACt                                                                                                                                                  >  6:270371/1‑149 (MQ=255)
  cgactGTGAGTTCCGCTACCACGGGCCAGCCTCGGATGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGc                                                                                                                                                <  7:133467/149‑1 (MQ=255)
   gactGTGAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCa                                                                                                                                               <  3:95746/149‑1 (MQ=255)
   gactGTGAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCa                                                                                                                                               <  7:335657/149‑1 (MQ=255)
     ctGTGAGTTCCGCTACCACGGGCCCGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAgg                                                                                                                                             <  8:281430/149‑1 (MQ=255)
      tgtgAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGc                                                                                                                                            >  8:62579/1‑149 (MQ=255)
      tgtgAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGc                                                                                                                                            >  2:226728/1‑149 (MQ=255)
        tgAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCg                                                                                                                                          >  2:379813/1‑149 (MQ=255)
               cGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTAGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGt                                                                                                                                   >  8:14788/1‑149 (MQ=255)
                gCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTg                                                                                                                                  >  5:290354/1‑149 (MQ=255)
                    ccACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGc                                                                                                                                <  8:19018/147‑1 (MQ=255)
                               ccTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGc                                                                                                                   <  7:117872/149‑1 (MQ=255)
                               ccTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCAGTACGGCAGGcgg                                                                                                                                          <  1:298665/126‑3 (MQ=255)
                               ccTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCAGTACGGCAGGcgg                                                                                                                                          >  2:298665/1‑124 (MQ=255)
                                    gACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTg                                                                                                              >  1:200745/1‑149 (MQ=255)
                                       gcgcTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGCCCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGca                                                                                                           >  5:20330/1‑149 (MQ=255)
                                              aGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGccc                                                                                                     >  8:200693/1‑148 (MQ=255)
                                                              tGGGCTGGTCGCCTACGTCGGGACCTTCTCAAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTggg                                                                                    >  3:111453/1‑149 (MQ=255)
                                                                     ggcGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGGTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGgc                                                                             <  1:308897/147‑1 (MQ=255)
                                                                     gTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTgg                                                                              <  7:62579/148‑1 (MQ=255)
                                                                     gTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTg                                                                               <  1:226728/147‑1 (MQ=255)
                                                                      tCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGgcg                                                                            >  5:64612/1‑149 (MQ=255)
                                                                           tACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCtt                                                                       >  7:210704/1‑149 (MQ=255)
                                                                             cGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTcc                                                                     <  7:143666/149‑1 (MQ=255)
                                                                              gTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCt                                                                    <  1:149693/149‑1 (MQ=255)
                                                                                 gggACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTgg                                                                 >  4:27233/1‑149 (MQ=255)
                                                                                     ccTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGATTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGaa                                                             <  5:270371/149‑1 (MQ=255)
                                                                                      cTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAg                                                            <  7:200693/149‑1 (MQ=255)
                                                                                       ttCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAgg                                                           >  2:373507/1‑149 (MQ=255)
                                                                                       ttCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAg                                                            >  6:118176/1‑148 (MQ=255)
                                                                                         ctcCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAg                                                            <  5:118176/146‑1 (MQ=255)
                                                                                         ctcCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGc                                                          <  6:290354/148‑1 (MQ=255)
                                                                                            cAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCAc                                                       <  4:111453/148‑1 (MQ=255)
                                                                                             aaGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCt                                                     >  5:326238/1‑149 (MQ=255)
                                                                                                aCCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGaa                                                  >  5:12525/1‑149 (MQ=255)
                                                                                                aCCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGaa                                                  <  7:14788/149‑1 (MQ=255)
                                                                                                      ctgcCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGca                                            <  3:27233/149‑1 (MQ=255)
                                                                                                      ctgcCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGc                                             <  1:379813/148‑1 (MQ=255)
                                                                                                           cGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATcc                                       >  1:75689/1‑149 (MQ=255)
                                                                                                                      tGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCAc                            >  4:202723/1‑149 (MQ=255)
                                                                                                                        ggCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGa                          <  2:75689/149‑1 (MQ=255)
                                                                                                                         gCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAg                         <  6:64612/149‑1 (MQ=255)
                                                                                                                         gCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAg                         >  2:53710/1‑149 (MQ=255)
                                                                                                                           tATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGt                       >  4:7426/1‑149 (MQ=255)
                                                                                                                             tGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCt                     <  6:20330/149‑1 (MQ=255)
                                                                                                                               ccGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTa                    >  1:368946/1‑148 (MQ=255)
                                                                                                                                cGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACa                  <  8:210704/149‑1 (MQ=255)
                                                                                                                                cGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACa                  <  8:143865/149‑1 (MQ=255)
                                                                                                                                 gTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAg                 >  4:47912/1‑149 (MQ=255)
                                                                                                                                    ttACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGAGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAgcg               >  6:306477/1‑148 (MQ=255)
                                                                                                                                     tACCGCCTGCGGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCa             >  7:219547/1‑149 (MQ=255)
                                                                                                                                       cggCCTGCAGTACGGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCAcg           >  6:5283/3‑149 (MQ=255)
                                                                                                                                        cGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGACGCTGCCACGAGGTCTACAGCGCAcgc          >  3:180567/1‑149 (MQ=255)
                                                                                                                                         gCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCAcgcc         >  6:62032/1‑149 (MQ=255)
                                                                                                                                             gCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCAcgccgc       >  1:16838/1‑147 (MQ=255)
                                                                                                                                               cGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAgc   <  3:202723/149‑1 (MQ=255)
                                                                                                                                               cGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAgc   >  5:144664/1‑149 (MQ=255)
                                                                                                                                               agTACGGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAgc   <  5:5283/148‑1 (MQ=255)
                                                                                                                                                gTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCATAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAgcg  >  7:7420/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TACGACTGTGAGTTCCGCTACCACGGGCCAGCCTCGGACGCGCTGCAGCGTCTGGACCGGCATGGGCTGGTCGCCTACGTCGGGACCTTCTCCAAGACCTTGCTGCCCGAGCTGCGCCTGGGCTATGCCGTATTACCGCCTGCCGTACTGCAGGCCGCTTGCGTGGCCAAGCACCTTAGCGACTGGCACAGCCCTACCCTGCTGCAGTGGGCGCTGGCGCGCTTCCTTGGCGAAGGCCACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCG  >  NC_002947/4279665‑4279957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑