Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,337,466	C→G	7.5%	L36V (CTC→GTC) ‡	PP_3812 →	lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,337,466	0	C	G	7.5%	194.4 / 4.2	63	L36V (CTC→GTC) ‡	PP_3812	lipase
Reads supporting (aligned to +/- strand):  ref base C (28/30);  new base G (2/3);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGACGCCCACTACCAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGATTCAGGCCAATGGCCA  >  NC_002947/4337318‑4337614                                                                                                                                                       |                                                                                                                                                         cAAGACGCCCACTACCAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCTGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTcc                                                                                                                                                          <  1:284260/149‑1 (MQ=255)  aaGACGCCCACTACCAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCt                                                                                                                                                         <  7:92564/149‑1 (MQ=255)   aGACGCCCACTACCAACAATTGCACAGACACGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCAACTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCATCCCTTTTGTCGAAAAACACACCCAGTCCTTCCTc                                                                                                                                                        <  1:233080/149‑1 (MQ=255)      cGCCCACTACCAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCTGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGaa                                                                                                                                                     <  1:89618/149‑1 (MQ=255)      cGCCCACTACCAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGaa                                                                                                                                                     <  4:73937/149‑1 (MQ=255)         ccACTACCAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGcc                                                                                                                                                  >  8:327793/1‑149 (MQ=255)               ccAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑ga                                                                                                                                           <  7:324300/148‑1 (MQ=255)                cAACACTTGCACAGAAAAGGATTCGCCCATTAACATTGTCCACAAAGCCTACTTACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑ga                                                                                                                                           <  1:122815/149‑1 (MQ=255)                         cacaGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGt                                                                                                                                 <  8:133119/148‑1 (MQ=255)                         cacaGAAAAGGATTCGCCCATGAACAATGTCCACAACGCC‑‑CTCACCACCTCCCTCTTGGCCATGACCGTTTCCAGTGCCTTCGCCGCAGCCAGCCCTGGTGTCGAACAACACACCCACGCCTTACTCGAAGCCCTT‑‑GAGAAAGGGCGTg                                                                                                                                >  6:76865/1‑149 (MQ=255)                                aaGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGcc                                                                                                                         >  4:290451/1‑149 (MQ=255)                                 aGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCCAGCCg                                                                                                                        >  8:151049/1‑149 (MQ=255)                                        gCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAAc                                                                                                                 <  3:230823/149‑1 (MQ=255)                                          ccATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGGCTTCGAGGAAGGCCTg‑‑gg                                                                                                                                           <  3:327556/121‑2 (MQ=255)                                          ccATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGGCTTCGAGGAAGGCCTg‑‑gg                                                                                                                                           >  4:327556/1‑120 (MQ=255)                                           cATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGc                                                                                                              >  4:323125/1‑149 (MQ=255)                                               aaCATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTca                                                                                                           <  3:290451/148‑1 (MQ=255)                                                 cATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCacac                                                                                                        <  7:221962/149‑1 (MQ=255)                                                 cATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCacac                                                                                                        <  2:87753/149‑1 (MQ=255)                                                  aTTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCacac                                                                                                        >  5:26757/1‑148 (MQ=255)                                                      tCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGGCTTCGAGGAAGGCCTg‑‑ggtgtg                                                                                                                                       >  1:314893/1‑108 (MQ=38)                                                      tCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGGCTTCGAGGAAGGCCTg‑‑ggtgtg                                                                                                                                       <  2:314893/113‑6 (MQ=38)                                                       ccACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAac                                                                                                   >  6:128018/1‑148 (MQ=255)                                                        cacaAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAacgt                                                                                                 >  2:211184/1‑148 (MQ=255)                                                        cacaAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAacga                                                                                                 >  8:171611/1‑149 (MQ=255)                                                         acaAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAacgac                                                                                                >  2:51232/1‑149 (MQ=255)                                                         acaAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAacgac                                                                                                <  1:36278/149‑1 (MQ=255)                                                           aaaGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGc                                                                                              <  7:327793/149‑1 (MQ=255)                                                           aaaGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGc                                                                                              >  4:117681/1‑149 (MQ=255)                                                                c‑‑cTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTg                                                                                                                     >  8:61034/1‑121 (MQ=255)                                                                c‑‑cTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTg                                                                                                                     <  7:61034/121‑1 (MQ=255)                                                                      accaccTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCgg                                                                                      >  3:286037/1‑148 (MQ=255)                                                                                      cccTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGTCGCCCGCGCGGTACTGA‑CCG‑GTGCCCAg                                                                   <  1:211184/149‑1 (MQ=255)                                                                                        cTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGc                                                                 >  6:136591/1‑149 (MQ=255)                                                                                             cGTTTCCAGTGCCTTCGCCTCATGCAGCCCTGGTGTCGTACAACACACCCAGGCCTTCGTCGAATCCCTG‑‑GAGAAATGGGTTGGCAAGCCGCTGTAACAGCTCACACCAAACTACGCCCGCGCGGTACTGA‑CCT‑GTGCCCAGGCTTCg                                                             <  2:62061/148‑1 (MQ=255)                                                                                             cGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCg                                                             <  7:151049/148‑1 (MQ=255)                                                                                              gTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGt                                                           <  8:108345/149‑1 (MQ=255)                                                                                               tttCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGt                                                           >  6:2605/1‑148 (MQ=255)                                                                                                  ccAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAg                                                       >  4:344392/1‑149 (MQ=255)                                                                                                   cAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCACGCCGCGGGAACAGCTCCCACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAgg                                                      >  7:159182/1‑149 (MQ=255)                                                                                                    aGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGt                                                     >  4:320319/1‑149 (MQ=255)                                                                                                        ccTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGAc                                                 <  3:117681/149‑1 (MQ=255)                                                                                                        ccTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGAc                                                 <  5:128018/149‑1 (MQ=255)                                                                                                          ttCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTGGAGAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGAc                                                 >  1:108822/1‑149 (MQ=255)                                                                                                              ccgcAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCtt                                                               >  3:71777/1‑129 (MQ=255)                                                                                                              ccgcAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCtt                                                               <  4:71777/129‑1 (MQ=255)                                                                                                                 caggcagCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCgggg                                        <  7:171611/149‑1 (MQ=255)                                                                                                                       gCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAg                                  <  1:51232/149‑1 (MQ=255)                                                                                                                                tCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAgg                                                      <  2:80683/120‑1 (MQ=255)                                                                                                                                tCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAgg                                                      >  1:80683/1‑120 (MQ=255)                                                                                                                                  gAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCGTGTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAAc                        <  1:94576/149‑1 (MQ=255)                                                                                                                                  gAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACg                       >  4:290944/1‑149 (MQ=255)                                                                                                                                   aacaacACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACg                       >  1:369159/1‑148 (MQ=255)                                                                                                                                      aacaCACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGACCCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAgg                                 <  1:263617/136‑1 (MQ=255)                                                                                                                                      aacaCACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAgg                                 >  2:263617/1‑135 (MQ=255)                                                                                                                                      aacaCACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAAc                        >  2:371234/1‑144 (MQ=255)                                                                                                                                      aacaCACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAAc                        <  1:371234/144‑1 (MQ=255)                                                                                                                                         acacCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGAt                 >  6:93322/1‑148 (MQ=255)                                                                                                                                           acCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGAtt                >  1:143823/1‑147 (MQ=255)                                                                                                                                           acCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGATTCa              <  5:76865/149‑1 (MQ=255)                                                                                                                                            cccAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGGCCGCGCGGTACTGA‑CCG‑GTGCCGAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGATTCAg             <  3:144349/149‑1 (MQ=255)                                                                                                                                              cAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGaa                             >  7:296477/1‑131 (MQ=255)                                                                                                                                              cAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGaa                             <  8:296477/131‑1 (MQ=255)                                                                                                                                                   cTTCCTCGAAGCCCTT‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCACGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGATTCAGGCCAATg      <  8:167497/149‑1 (MQ=255)                                                                                                                                                       cTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGATTCAGGCCAATGGc    >  5:328328/1‑147 (MQ=255)                                                                                                                                                       cTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGATTCAGGCCAATGGCCa  <  6:26757/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                         CAAGACGCCCACTACCAACACTTGCACAGAAAAGGATTCGCCCATGAACATTGTCCACAAAGCC‑‑CTCACCACCTCCCTCTTGGCCCTGTCCGTTTCCAGTGCCTTCGCCGCAGGCAGCCCTGGTGTCGAACAACACACCCAGGCCTTCCTCGAAGCCCTG‑‑GAGAAAGGGGGTGGCAAGCCGCTGGAACAGCTCACACCAAACGACGCCCGCGCGGTACTGA‑CCG‑GTGCCCAGGCTTCGGTAAAGGTCGACCTATCGGGGGTCGAGGTGAAGGAACGCACGATTCAGGCCAATGGCCA  >  NC_002947/4337318‑4337614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑