Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,344,304	C→T	13.0%	intergenic (+35/‑121)	PP_3817 → / → PP_3818	polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,344,304	0	C	T	13.0%	110.1 / 7.0	46	intergenic (+35/‑121)	PP_3817/PP_3818	polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base T (2/4);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCT  >  NC_002947/4344162‑4344450                                                                                                                                               |                                                                                                                                                   ccGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCAccgc                                                                                                                                               <  5:141072/148‑1 (MQ=255)         gCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCgg                                                                                                                                       >  5:118748/1‑148 (MQ=255)              gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACg                                                                                                                                 <  2:123718/149‑1 (MQ=255)                 aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATc                                                                                                                              >  5:95023/1‑149 (MQ=38)                 aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATc                                                                                                                              >  2:9154/1‑149 (MQ=255)                  cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCt                                                                                                                             >  8:232196/1‑149 (MQ=38)                   aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTg                                                                                                                            <  6:95023/149‑1 (MQ=37)                   aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTg                                                                                                                            <  2:304030/149‑1 (MQ=37)                   aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTg                                                                                                                            >  1:42406/1‑149 (MQ=255)                      gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAAATAGAGCCACGCCCGCGCGGCCCACGaaatgg                                                                                                                           >  6:257332/1‑142 (MQ=255)                      gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCa                                                                                                                         <  3:180091/149‑1 (MQ=37)                      gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTAAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                           >  3:154969/1‑147 (MQ=255)                            gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCt                                                                                                                             >  6:148662/1‑139 (MQ=255)                            gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCt                                                                                                                             <  5:148662/139‑1 (MQ=255)                             tGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGGCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTg                                                                                                                  <  3:86260/149‑1 (MQ=255)                              gCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGc                                                                                                                 <  7:232196/149‑1 (MQ=25)                                 ttGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCGTAGCGCGACCTGTGCAAGTCGGCCGGGGCGCATTACCGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCGGCGCGGCACACGATCTGGCTGGCGCTGCACt                                                                                                              <  8:349730/149‑1 (MQ=255)                                     gCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTg                                                                                                          >  2:234102/1‑149 (MQ=255)                                              gACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACAGGATCTGGCAGGCGCTGCACTATTGGAGGCGTAc                                                                                                 <  6:118748/149‑1 (MQ=255)                                                     gCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCATGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGt                                                                                          >  1:351317/1‑149 (MQ=255)                                                       aTTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTca                                                                                        >  5:196844/1‑149 (MQ=255)                                                           ataCCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACAt                                                                                     >  4:312943/1‑148 (MQ=255)                                                             aCCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTat                                                                                  >  2:59619/1‑149 (MQ=255)                                                             aCCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTa                                                                                   >  4:41713/1‑148 (MQ=255)                                                                 aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCAGCCAGAACCAACACCCCCCCCCCGGCAAACCATAAGGCACGCCCAGCACTATTTGAGGCGTACGCCCCCTCACCTTataaa                                                                               >  1:303626/1‑145 (MQ=255)                                                                       ccGAGAGCGACGTGAGCAAGGCGGCGGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACTATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCa                                                                        <  6:196844/149‑1 (MQ=255)                                                                           gagCGACTTAAGCATGTGGGCGGGGGCGCTTTAACGCTCTTTTAGGTTGCAGCGCCTGCCATATCGAGCCCCGCCCGCGCGGCACACGATCTGGCTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTg                                                                     <  4:154969/148‑1 (MQ=255)                                                                                 ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAAt                                                              <  2:42406/149‑1 (MQ=255)                                                                                       cAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGcc                                                         <  3:41713/148‑1 (MQ=255)                                                                                              gCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAAct                                                 >  5:92526/1‑149 (MQ=255)                                                                                                  gggCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTAt                                             <  3:312943/149‑1 (MQ=255)                                                                                                   ggCGAATTAACGTTCTTGTAGGTTGCAGCGCCGGCCTTATCGAGCACCGCCGGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATg                                            <  2:351317/149‑1 (MQ=255)                                                                                                        aTTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                       <  1:234102/149‑1 (MQ=255)                                                                                                             cGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTAc                                                                                                 <  4:66286/86‑1 (MQ=255)                                                                                                             cGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTAc                                                                                                 >  3:66286/1‑86 (MQ=255)                                                                                                              gCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAc                                 >  8:179871/1‑149 (MQ=255)                                                                                                                  ttGTACGTTGCAGCGCCTGCCTGAGCCCGCACCGCCCGCGGGCCAACCTATCGGGCTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCg                              <  5:257332/148‑1 (MQ=255)                                                                                                                     tACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCa                           <  6:92526/148‑1 (MQ=255)                                                                                                                            cAGCGCCTGCCAGACCGACCACCGCCCGCGCGGCACACGATCTGGCTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGc                   <  1:9154/149‑1 (MQ=255)                                                                                                                                   tGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTa                                                                                   <  1:189999/78‑1 (MQ=255)                                                                                                                                   tGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTa                                                                                   >  2:189999/1‑78 (MQ=255)                                                                                                                                    gCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCAAACTCTAAGCCACAGGTCACCCGGCATGCTCCGCCTGCATg            >  8:323471/1‑148 (MQ=255)                                                                                                                                      cAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt         >  4:169080/1‑149 (MQ=255)                                                                                                                                       aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGATCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt        >  1:109406/1‑149 (MQ=255)                                                                                                                                        gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGTCATGCTCCGCCTGCTTGTCTtg       >  8:171668/1‑149 (MQ=255)                                                                                                                                             aGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTgctgct  <  5:334648/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   CCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCT  >  NC_002947/4344162‑4344450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑