Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,344,321 A→T 17.8% intergenic (+52/‑104) PP_3817 → / → PP_3818 polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,344,3210AT17.8% 98.9 / 15.3 50intergenic (+52/‑104)PP_3817/PP_3818polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (22/18);  new base T (4/5);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

GCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTCG  >  NC_002947/4344175‑4344467
                                                                                                                                                  |                                                                                                                                                  
gCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACg                                                                                                                                                  <  2:123718/149‑1 (MQ=255)
   aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATc                                                                                                                                               >  5:95023/1‑149 (MQ=38)
   aCAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATc                                                                                                                                               >  2:9154/1‑149 (MQ=255)
    cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCt                                                                                                                                              >  8:232196/1‑149 (MQ=38)
     aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTg                                                                                                                                             <  6:95023/149‑1 (MQ=37)
     aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTg                                                                                                                                             <  2:304030/149‑1 (MQ=37)
     aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTg                                                                                                                                             >  1:42406/1‑149 (MQ=255)
        gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTTCCAAATAGAGCCACGCCCGCGCGGCCCACGaaatgg                                                                                                                                            >  6:257332/1‑142 (MQ=255)
        gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCa                                                                                                                                          <  3:180091/149‑1 (MQ=37)
        gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTAAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTgg                                                                                                                                            >  3:154969/1‑147 (MQ=255)
              gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCt                                                                                                                                              <  5:148662/139‑1 (MQ=255)
              gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCt                                                                                                                                              >  6:148662/1‑139 (MQ=255)
               tGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGGCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTg                                                                                                                                   <  3:86260/149‑1 (MQ=255)
                gCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGc                                                                                                                                  <  7:232196/149‑1 (MQ=25)
                   ttGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCGTAGCGCGACCTGTGCAAGTCGGCCGGGGCGCATTACCGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCGGCGCGGCACACGATCTGGCTGGCGCTGCACt                                                                                                                               <  8:349730/149‑1 (MQ=255)
                       gCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTg                                                                                                                           >  2:234102/1‑149 (MQ=255)
                                gACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACAGGATCTGGCAGGCGCTGCACTATTGGAGGCGTAc                                                                                                                  <  6:118748/149‑1 (MQ=255)
                                       gCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCATGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGt                                                                                                           >  1:351317/1‑149 (MQ=255)
                                         aTTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTca                                                                                                         >  5:196844/1‑149 (MQ=255)
                                             ataCCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACAt                                                                                                      >  4:312943/1‑148 (MQ=255)
                                               aCCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTat                                                                                                   >  2:59619/1‑149 (MQ=255)
                                               aCCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTa                                                                                                    >  4:41713/1‑148 (MQ=255)
                                                   aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCAGCCAGAACCAACACCCCCCCCCCGGCAAACCATAAGGCACGCCCAGCACTATTTGAGGCGTACGCCCCCTCACCTTataaa                                                                                                >  1:303626/1‑145 (MQ=255)
                                                         ccGAGAGCGACGTGAGCAAGGCGGCGGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACTATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCa                                                                                         <  6:196844/149‑1 (MQ=255)
                                                             gagCGACTTAAGCATGTGGGCGGGGGCGCTTTAACGCTCTTTTAGGTTGCAGCGCCTGCCATATCGAGCCCCGCCCGCGCGGCACACGATCTGGCTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTg                                                                                      <  4:154969/148‑1 (MQ=255)
                                                                   ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAAt                                                                               <  2:42406/149‑1 (MQ=255)
                                                                         cAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGcc                                                                          <  3:41713/148‑1 (MQ=255)
                                                                                gCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAAct                                                                  >  5:92526/1‑149 (MQ=255)
                                                                                    gggCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTAt                                                              <  3:312943/149‑1 (MQ=255)
                                                                                     ggCGAATTAACGTTCTTGTAGGTTGCAGCGCCGGCCTTATCGAGCACCGCCGGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATg                                                             <  2:351317/149‑1 (MQ=255)
                                                                                          aTTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCcaca                                                        <  1:234102/149‑1 (MQ=255)
                                                                                               cGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTAc                                                                                                                  <  4:66286/86‑1 (MQ=255)
                                                                                               cGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTAc                                                                                                                  >  3:66286/1‑86 (MQ=255)
                                                                                                gCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAc                                                  >  8:179871/1‑149 (MQ=255)
                                                                                                    ttGTACGTTGCAGCGCCTGCCTGAGCCCGCACCGCCCGCGGGCCAACCTATCGGGCTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCg                                               <  5:257332/148‑1 (MQ=255)
                                                                                                       tACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCa                                            <  6:92526/148‑1 (MQ=255)
                                                                                                              cAGCGCCTGCCAGACCGACCACCGCCCGCGCGGCACACGATCTGGCTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGc                                    <  1:9154/149‑1 (MQ=255)
                                                                                                                     tGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTa                                                                                                    >  2:189999/1‑78 (MQ=255)
                                                                                                                     tGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTa                                                                                                    <  1:189999/78‑1 (MQ=255)
                                                                                                                      gCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCAAACTCTAAGCCACAGGTCACCCGGCATGCTCCGCCTGCATg                             >  8:323471/1‑148 (MQ=255)
                                                                                                                        cAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCt                          >  4:169080/1‑149 (MQ=255)
                                                                                                                         aGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGATCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCtt                         >  1:109406/1‑149 (MQ=255)
                                                                                                                          gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGTCATGCTCCGCCTGCTTGTCTtg                        >  8:171668/1‑149 (MQ=255)
                                                                                                                              gtgtgccgcgcggGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAccc                                                >  8:26329/6‑121 (MQ=37)
                                                                                                                              gtgtgccgcgcggGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                                >  2:338769/6‑137 (MQ=38)
                                                                                                                              gtgtgccgcgcggGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                                <  1:338769/132‑1 (MQ=38)
                                                                                                                               aGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTgctgct                   <  5:334648/149‑1 (MQ=255)
                                                                                                                                   ccgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCAcc               <  1:59619/149‑1 (MQ=255)
                                                                                                                                         gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTa         >  3:109996/1‑149 (MQ=255)
                                                                                                                                         gcgcgGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTa         >  7:196764/1‑149 (MQ=255)
                                                                                                                                               cacaCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTc   >  1:245037/1‑149 (MQ=255)
                                                                                                                                               cacaCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTc   <  8:196764/149‑1 (MQ=255)
                                                                                                                                               cacaCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTc   <  4:109996/149‑1 (MQ=255)
                                                                                                                                                acacGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTcg  >  7:308032/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTCG  >  NC_002947/4344175‑4344467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑