Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,346,097	G→A	5.5%	P332L (CCG→CTG)	gor ←	glutathione reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,346,097	0	G	A	5.5%	216.0 / 2.9	73	P332L (CCG→CTG)	gor	glutathione reductase
Reads supporting (aligned to +/- strand):  ref base G (35/34);  new base A (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GTCGGTCAGGGTCAGCTTCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCGGAT  >  NC_002947/4345949‑4346242                                                                                                                                                     |                                                                                                                                                    gTCGGTCAGGGTCAGCTTCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACAGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCg                                                                                                                                                     >  8:282378/1‑149 (MQ=255)        agggtcagCTTCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTa                                                                                                                                              <  4:175772/149‑1 (MQ=255)          ggtcagCTTCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGGTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCAACCGGGCGGTACt                                                                                                                                            >  5:383914/1‑149 (MQ=255)          ggtcagCTTCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACt                                                                                                                                            >  5:265866/1‑149 (MQ=255)                cTTCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCgg                                                                                                                                      >  4:77592/1‑149 (MQ=255)                  tCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCCGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGGTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCTCCGGGCGGTTCTGGTAgggg                                                                                                                                    >  6:258525/1‑148 (MQ=255)                  tCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCgg                                                                                                                                      <  3:195951/147‑1 (MQ=255)                  tCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGATCTGGTAATACACCGGGGGGTAATTCTCGGGt                                                                                                                                    >  6:258527/1‑149 (MQ=255)                  tCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCAGCTCTTCGGACAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGCGATGTTCTGGTAATCCACCGGGCGGTACTGCTCgg                                                                                                                                      >  4:195951/1‑147 (MQ=255)                   cATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGt                                                                                                                                    <  1:106649/148‑1 (MQ=255)                       gCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGa                                                                                                                                <  2:374847/148‑1 (MQ=255)                             aaGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGgcg                                                                                                                         >  7:41977/1‑149 (MQ=255)                                        gAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACgg                                                                                                              >  5:59042/1‑149 (MQ=255)                                        gAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGCGGATGCTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACgg                                                                                                              <  5:258527/149‑1 (MQ=255)                                        gAAAACCTGCACCTTCTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCACGCCTACGGTACCGATGGGTGGCTGGCTGACCACCGCGGTGTGGATGTTCGGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACgc                                                                                                              <  5:258525/149‑2 (MQ=255)                                              cTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGc                                                                                                        <  8:54380/149‑1 (MQ=255)                                              cTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGc                                                                                                        >  1:356502/1‑149 (MQ=255)                                              cTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGGTTGAACAGGCGCCGCGCCACGGCCATGc                                                                                                        >  3:289359/1‑149 (MQ=255)                                               tGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGcc                                                                                                       >  7:146153/1‑149 (MQ=255)                                                   ccTTGTGCCCGTCATCCAGCGCCTGCTCTTCGGTCAAGCCTAGGGTAGCGATGGGTGGCTGGCTGAACACCGCGGTGTGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCa                                                                                                   <  8:125879/149‑1 (MQ=255)                                                   ccTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCtt                                                                                                     >  5:261854/1‑147 (MQ=255)                                                   ccTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCa                                                                                                   <  7:335250/149‑1 (MQ=255)                                                    cTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTAc                                                                                                                                             <  6:225009/106‑1 (MQ=255)                                                    cTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTAc                                                                                                                                             >  5:225009/1‑106 (MQ=255)                                                    cTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAg                                                                                                  >  5:130060/1‑149 (MQ=255)                                                    cTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAg                                                                                                  >  1:252939/1‑149 (MQ=255)                                                       gtgCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGcc                                                                                                >  7:285791/1‑148 (MQ=255)                                                           ccGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCa                                                                                                   >  5:57032/1‑141 (MQ=255)                                                           ccGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCa                                                                                                   <  6:57032/141‑1 (MQ=255)                                                            cGGCCTCCAGCGCCTTCTCTTCGGTCAAGCCTACGGTACCGATGGGTGTCTGGCTTAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCTCCACGGCCATGCCTTCAGCCAA‑‑GGcc                                                                                        <  6:383914/149‑1 (MQ=255)                                                                cTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTg                                                                                    <  3:115429/149‑1 (MQ=255)                                                                   cAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAC‑‑GGCCACTgggg                                                                                 >  2:114039/1‑149 (MQ=255)                                                                   cAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTggg                                                                                  <  6:130060/148‑1 (MQ=255)                                                                   cAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTgg                                                                                   <  6:59042/147‑1 (MQ=255)                                                                    aGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGt                                                                                <  7:282378/149‑1 (MQ=255)                                                                     gcgcCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTc                                                                               <  6:265866/149‑1 (MQ=255)                                                                           gCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTg                                                                          >  2:63475/1‑148 (MQ=255)                                                                           gCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTg                                                                          >  2:317912/1‑148 (MQ=255)                                                                           gCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGAGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTg                                                                          >  4:272874/1‑148 (MQ=255)                                                                            ctctTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTg                                                                          <  4:168330/147‑1 (MQ=255)                                                                               ttCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTggg                                                                                  <  3:130867/136‑1 (MQ=255)                                                                               ttCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTggg                                                                                  >  4:130867/1‑136 (MQ=255)                                                                                 cGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCAcgc                                                                     <  1:340317/147‑1 (MQ=255)                                                                                      aaGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTggg                                                                                  <  4:292494/129‑1 (MQ=255)                                                                                      aaGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTggg                                                                                  >  3:292494/1‑129 (MQ=255)                                                                                              ggTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGc                                                      <  1:317912/149‑1 (MQ=255)                                                                                                         gTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCgag                                            >  5:16458/1‑148 (MQ=255)                                                                                                            gctggctgAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATc                                        <  4:244387/149‑1 (MQ=255)                                                                                                              tggctgAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATc                                        >  1:343154/1‑147 (MQ=255)                                                                                                                gctgAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGTCCATGCCTTCAGCCAA‑‑GTCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGa                                      <  2:343154/147‑1 (MQ=255)                                                                                                                gctgAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAg                                     <  3:272874/148‑1 (MQ=255)                                                                                                                  tgAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGgc                                  >  7:61832/1‑149 (MQ=255)                                                                                                                    aaCACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGACACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAAGCGAGGtcgc                                >  4:5472/1‑149 (MQ=255)                                                                                                                        ccGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCggtg                            <  3:77592/149‑1 (MQ=255)                                                                                                                          gcgGGGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCCATTGGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCggtg                            <  1:114039/149‑1 (MQ=255)                                                                                                                            ggTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCt                        <  1:63475/149‑1 (MQ=255)                                                                                                                                gggATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGCGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTa                    >  5:113532/1‑149 (MQ=255)                                                                                                                                  gATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACt                  >  2:343479/1‑149 (MQ=255)                                                                                                                                  gATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGCTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATAGCCAATGGCGAGAATCGAGGGCGCGCTGGTCTGGTagt                  >  3:21746/1‑147 (MQ=255)                                                                                                                                    tGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCGGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGc                <  2:252939/149‑1 (MQ=255)                                                                                                                                     gTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGc                <  2:356502/148‑1 (MQ=255)                                                                                                                                         tGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATc           >  3:358683/1‑149 (MQ=255)                                                                                                                                          ggTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATc           >  1:362598/1‑148 (MQ=255)                                                                                                                                           gTAATCCACCAGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGa         >  4:329800/1‑149 (MQ=255)                                                                                                                                           gTAATCCACCAGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGa         >  4:42338/1‑149 (MQ=255)                                                                                                                                             aaTCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGAcc       >  6:326960/1‑149 (MQ=255)                                                                                                                                             aaTCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGAcc       >  4:274130/1‑149 (MQ=255)                                                                                                                                               tCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGCATCGAGGGAGCGGAGCTCTGGTACTCCTCATCCACCCg     >  5:22922/1‑149 (MQ=255)                                                                                                                                                 cACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCgg    <  2:362598/148‑1 (MQ=255)                                                                                                                                                  cccGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGCCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCGGAt  <  2:374318/148‑1 (MQ=255)                                                                                                                                                  aCCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCGGAt  <  6:129265/149‑1 (MQ=255)                                                                                                                                                  aCCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCGGAt  <  6:135389/149‑1 (MQ=255)                                                                                                                                                  aCCAGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCGGAt  <  3:42338/149‑1 (MQ=255)                                                                                                                                                  aCCAGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCGGAt  <  3:329800/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTCGGTCAGGGTCAGCTTCATGGCCCGGAAGCGGCTTTCGAATACCTGCACCTTGTGCCCGGCCTCCAGCGCCTGCTCTTCGGTCAAGCCTACGGTACCGATGGGTGGCTGGCTGAACACCGCGGTGGGGATGTTCTGGTAATCCACCGGGCGGTACTGCTCGGGTTTGAACAGGCGCCGCGCCACGGCCATGCCTTCAGCCAA‑‑GGCCACTGGGGTCAACTGCACGCGGCCGATCACATCGCCAATGGCGAGAATCGAGGGCGCGGTGGTCTGGTACTGCTCATCGACCCGGAT  >  NC_002947/4345949‑4346242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑