Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,375,590 A→C 9.9% intergenic (‑55/+55) PP_5637 ← / ← PP_3851 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,375,5900AC 9.9% 163.5 / 10.3 61intergenic (‑55/+55)PP_5637/PP_3851hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/30);  new base C (4/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01

TCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAG  >  NC_002947/4375446‑4375737
                                                                                                                                                |                                                                                                                                                   
tCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGtata                                                                                                                                                     >  1:100640/1‑145 (MQ=255)
  gACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATa                                                                                                                                               >  5:180588/1‑149 (MQ=255)
  gACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATa                                                                                                                                               <  2:224263/149‑1 (MQ=255)
    cccAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACa                                                                                                                                             <  5:73704/149‑1 (MQ=255)
     ccAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAg                                                                                                                                            >  6:203931/1‑149 (MQ=255)
         agGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATCTTTATACAGTAtt                                                                                                                                        <  1:243354/149‑2 (MQ=255)
         agGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATc                                                                                                                                        >  8:192915/1‑149 (MQ=255)
             tCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGta                                                                                                                                     >  4:150902/1‑148 (MQ=255)
                 tgtgATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAgat                                                                                                                                >  8:21329/1‑149 (MQ=255)
                         tCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAgatgccgatgc                                                                                                                        <  6:36449/149‑1 (MQ=255)
                         tCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAgatgccgatgc                                                                                                                        >  2:323346/1‑149 (MQ=255)
                               aTGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAgg                                                                                                                  <  1:129011/149‑1 (MQ=255)
                                     aCGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATc                                                                                                            >  2:106809/1‑149 (MQ=255)
                                            cgcgGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATc                                                                                                     <  5:203931/149‑1 (MQ=255)
                                              cgGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATcag                                                                                                   <  3:83479/149‑1 (MQ=255)
                                                gAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATcagca                                                                                                 <  2:188918/149‑1 (MQ=255)
                                                     gCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGAt                                                                                              >  6:197416/1‑147 (MQ=255)
                                                     gCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCa                                                                                            <  3:65611/149‑1 (MQ=255)
                                                     gCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCa                                                                                            <  6:180588/149‑1 (MQ=255)
                                                            tttCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAgg                                                                                     >  3:127551/1‑149 (MQ=255)
                                                               ccGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACGTGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGc                                                                                  >  4:77422/1‑149 (MQ=255)
                                                               ccGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAgggg                                                                                   <  7:21329/148‑1 (MQ=255)
                                                                cGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGTCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCg                                                                                 <  3:150902/149‑1 (MQ=255)
                                                                  tAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCgg                                                                                >  1:1260/1‑148 (MQ=255)
                                                                    ggCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGaa                                                                              <  2:6556/148‑1 (MQ=255)
                                                                             aGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAg                                                                                                                                                  >  5:295012/1‑71 (MQ=255)
                                                                             aGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAg                                                                                                                                                  <  6:295012/71‑1 (MQ=255)
                                                                             aGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCtt                                                                       <  8:40977/146‑1 (MQ=255)
                                                                              gggCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGccc                                                                   <  5:127482/149‑1 (MQ=255)
                                                                                  aaGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCa                                                               >  7:92180/1‑149 (MQ=255)
                                                                                   aGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCAt                                                              <  4:127551/149‑1 (MQ=255)
                                                                                        aCGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTgg                                                         >  4:49709/1‑149 (MQ=255)
                                                                                        aCGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTgg                                                         <  8:92180/149‑1 (MQ=255)
                                                                                         cGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGc                                                        <  2:1260/149‑1 (MQ=255)
                                                                                         cGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGc                                                        <  1:233806/149‑1 (MQ=255)
                                                                                             aTGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAAt                                                     >  5:331748/1‑148 (MQ=255)
                                                                                              tGCTCGCGGTGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCa                                                   >  5:268206/1‑149 (MQ=255)
                                                                                                cTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCAcc                                                 <  7:192915/149‑1 (MQ=255)
                                                                                                cTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCAc                                                  <  3:49709/148‑1 (MQ=255)
                                                                                                 tCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCAccc                                                >  6:65911/1‑149 (MQ=255)
                                                                                                            aaCGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGt                                       >  2:343009/1‑147 (MQ=255)
                                                                                                            aaCGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGt                                       <  1:343009/147‑1 (MQ=255)
                                                                                                            aaCGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTaa                                     >  8:53103/1‑149 (MQ=255)
                                                                                                                 gaaggaagTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGa                                >  8:106127/1‑149 (MQ=255)
                                                                                                                   aggaagTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGTGCAATCGCCGATCAGGAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGaca                              <  2:100640/149‑1 (MQ=255)
                                                                                                                    ggaagTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGacac                             >  5:218220/1‑149 (MQ=255)
                                                                                                                    ggaagTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGacac                             <  1:130745/149‑1 (MQ=255)
                                                                                                                        gTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGaa                                                                                       >  6:174708/1‑87 (MQ=255)
                                                                                                                        gTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGaa                                                                                       <  5:174708/87‑1 (MQ=255)
                                                                                                                           cATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGa                      <  1:106809/149‑1 (MQ=255)
                                                                                                                               cGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAAc                   <  5:345776/148‑1 (MQ=255)
                                                                                                                                 tatacgaTACTGTATCTTTATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACa                  >  8:353340/7‑147 (MQ=255)
                                                                                                                                     cAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGt                                                                                          >  8:162846/1‑71 (MQ=255)
                                                                                                                                     cAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGt                                                                                          <  7:162846/71‑1 (MQ=255)
                                                                                                                                      gaTACTGTATCTTTATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAAc                   <  5:358556/140‑1 (MQ=255)
                                                                                                                                      gaTACTGTATCTTTATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAAc                   >  6:358556/2‑141 (MQ=255)
                                                                                                                                      gaTACTGTATCTTTATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTc           >  8:345958/2‑149 (MQ=255)
                                                                                                                                      gaTACTGTATCTTTATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTc           >  5:47962/2‑149 (MQ=255)
                                                                                                                                      aaTACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTc           <  6:331748/149‑1 (MQ=255)
                                                                                                                                           tGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGAcaccac                          >  7:325546/1‑129 (MQ=255)
                                                                                                                                           tGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGAcaccac                          <  8:325546/129‑1 (MQ=255)
                                                                                                                                           tGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGc        >  3:328327/1‑147 (MQ=255)
                                                                                                                                               taAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCcagcag  >  2:50514/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAAGATACAGTATCGTATAGATGCCGATGCCGCAGGGCAATCGCCGATCAGCAGATCAGTGAAGGGGCGGAAGCTCCTTGCCCCGCATCTTGGCAATCACCCGAAGCTGGTAATCGGACACCACCTGAAACAGAGACTCGGCCAGCAG  >  NC_002947/4375446‑4375737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑