Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,446,491 G→C 8.1% Y358* (TAC→TAG)  nicC ← 6‑hydroxynicotinate 3‑monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,446,4910GC 8.1% 195.2 / 6.5 62Y358* (TAC→TAG) nicC6‑hydroxynicotinate 3‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (33/24);  new base C (2/3);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CCCTTGCCGCCATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGATGGC  >  NC_002947/4446343‑4446637
                                                                                                                                                     |                                                                                                                                                  
cccTTGCCGCCATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTg                                                                                                                                                    <  5:169401/149‑1 (MQ=255)
 ccTTGCCGCCATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGt                                                                                                                                                   <  4:108905/149‑1 (MQ=255)
     gccgccATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTagag                                                                                                                                               >  4:83934/1‑149 (MQ=255)
       cgccATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTCTACAGc                                                                                                                                              >  1:224863/1‑148 (MQ=255)
         ccATAGCGCAGGTAATGCTGGCGGAGGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTATCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAg                                                                                                                                           <  2:91102/149‑1 (MQ=255)
          cATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTCTACAGCCAgg                                                                                                                                          <  4:53227/149‑1 (MQ=255)
           aTAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGgt                                                                                                                                         >  2:47389/1‑149 (MQ=255)
            tAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTCTACAGCCAggaa                                                                                                                                        <  2:224863/149‑3 (MQ=255)
                gcAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAACCCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTgg                                                                                                                                    >  8:159839/1‑149 (MQ=255)
                gcAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGCTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGCGGTCGTAGCCGGAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTgg                                                                                                                                    <  7:278283/149‑1 (MQ=255)
                    gTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGtt                                                                                                                                >  1:220720/1‑149 (MQ=255)
                    gTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGtt                                                                                                                                <  3:275241/149‑1 (MQ=255)
                     tAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTg                                                                                                                               <  2:42497/149‑1 (MQ=255)
                              cGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGAt                                                                                                                      <  1:305215/149‑1 (MQ=255)
                                      aTTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTg                                                                                                              >  1:187854/1‑149 (MQ=255)
                                      aTTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTg                                                                                                              >  3:185261/1‑149 (MQ=255)
                                                   ttACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAATCCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGctct                                                                                                 <  3:201570/149‑1 (MQ=255)
                                                        accaGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCg                                                                                            >  7:114373/1‑149 (MQ=255)
                                                        accaGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCg                                                                                            >  1:219639/1‑149 (MQ=255)
                                                          caGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGGCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGt                                                                                          <  7:349997/149‑1 (MQ=255)
                                                          caGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGt                                                                                          >  8:320241/1‑149 (MQ=255)
                                                          caGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGt                                                                                          >  1:345536/1‑149 (MQ=255)
                                                           aGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGtt                                                                                         <  7:320241/149‑1 (MQ=255)
                                                              gacgaAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGc                                                                                      <  7:159839/149‑1 (MQ=255)
                                                                  aaGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCa                                                                                  <  3:120096/149‑1 (MQ=255)
                                                                  aaGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCa                                                                                  <  7:235379/149‑1 (MQ=255)
                                                                   aGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCa                                                                                  >  6:372456/1‑148 (MQ=255)
                                                                   aGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCAt                                                                                 <  1:47389/149‑1 (MQ=255)
                                                                     gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATa                                                                                >  3:28655/1‑148 (MQ=255)
                                                                     gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATa                                                                                >  4:171387/1‑148 (MQ=255)
                                                                     gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATAc                                                                               <  3:83934/149‑1 (MQ=255)
                                                                      tGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATAc                                                                               >  6:347444/1‑148 (MQ=255)
                                                                           aTGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGAATATTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAAcgc                                                                          >  1:297132/1‑149 (MQ=255)
                                                                                   tCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAAcgcg                                                                         >  1:151839/1‑141 (MQ=255)
                                                                                      ccGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCg                                                              <  1:7135/149‑1 (MQ=255)
                                                                                       cGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCgg                                                             >  2:252288/1‑149 (MQ=255)
                                                                                        gCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCggt                                                            >  4:52087/1‑149 (MQ=255)
                                                                                          tttCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCggtgg                                                          >  6:244509/1‑149 (MQ=255)
                                                                                                           tAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGtctc                                         <  1:357536/149‑1 (MQ=255)
                                                                                                           tAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGtctc                                         <  5:347444/149‑1 (MQ=255)
                                                                                                             gagGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCt                                       >  8:212613/1‑149 (MQ=255)
                                                                                                             gagGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCt                                       >  5:36401/1‑149 (MQ=255)
                                                                                                                   gTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTgcagg                                  >  4:90054/1‑148 (MQ=255)
                                                                                                                        cGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCgg                            >  6:131339/1‑149 (MQ=255)
                                                                                                                         gTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCggg                           >  2:308288/1‑149 (MQ=255)
                                                                                                                           aaaCCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTg                                                                                              >  3:152711/1‑80 (MQ=255)
                                                                                                                           aaaCCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTg                                                                                              <  4:152711/80‑1 (MQ=255)
                                                                                                                            aaCCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTc                         <  2:219639/148‑1 (MQ=255)
                                                                                                                               ccAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCa                     >  3:66374/1‑149 (MQ=255)
                                                                                                                                 aGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATg                   <  3:227376/149‑1 (MQ=255)
                                                                                                                                  ggCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATgg                  <  3:178933/149‑1 (MQ=255)
                                                                                                                                   gCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGc                 <  5:131339/149‑1 (MQ=255)
                                                                                                                                   gCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGc                 >  8:193495/1‑149 (MQ=255)
                                                                                                                                      gg‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCcgc              >  5:32177/1‑149 (MQ=255)
                                                                                                                                          tCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTc           >  1:62830/1‑149 (MQ=255)
                                                                                                                                            ttCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCt         >  4:147924/1‑149 (MQ=255)
                                                                                                                                               cTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGa      <  4:66374/149‑1 (MQ=255)
                                                                                                                                               cTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGa      >  6:175235/1‑149 (MQ=255)
                                                                                                                                               cTGGCTCTACAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGcc                                                <  6:233344/107‑1 (MQ=255)
                                                                                                                                               cTGGCTCTACAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGcc                                                >  5:233344/1‑107 (MQ=255)
                                                                                                                                                tGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGAt     >  6:223440/1‑149 (MQ=255)
                                                                                                                                                 ggCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGATg    <  2:297132/149‑1 (MQ=255)
                                                                                                                                                    tGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGATGGc  >  2:268210/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCCTTGCCGCCATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGG‑ATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTGGATACCGATTGCACCTGCGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGATGGC  >  NC_002947/4446343‑4446637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑