Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,509,002	A→T	6.9%	S354T (TCG→ACG)	serS ←	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,509,002	0	A	T	6.9%	177.3 / 2.7	58	S354T (TCG→ACG)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (25/29);  new base T (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGTTTTCCAGCACAGCAACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGT  >  NC_002947/4508856‑4509149                                                                                                                                                   |                                                                                                                                                    gTAGTTTTCCAGCACAGCAACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGa                                                                                                                                                     <  2:298197/147‑1 (MQ=255) gTAGTTTTCCAGCACAGCAACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGc                                                                                                                                                   >  6:150037/1‑149 (MQ=255)    gTTTTCCAGCACAGCAACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGTGCTGa                                                                                                                                                >  6:282215/1‑149 (MQ=255)      tttCCAGCACAGCAACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCCGGAAGTCACCACAGTTGGAGCACGAGCTGAtt                                                                                                                                              >  4:82001/1‑149 (MQ=255)           agcacagcaACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGTGCTGATTTcgcg                                                                                                                                         >  5:37997/1‑149 (MQ=255)            gcacagcaACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCgg                                                                                                                                        <  2:345316/149‑1 (MQ=255)                 gcaACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTTGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACtt                                                                                                                                   <  3:267037/149‑1 (MQ=255)                  caACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTg                                                                                                                                  >  2:119539/1‑149 (MQ=255)                     ccAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTcc                                                                                                                               >  3:155349/1‑149 (MQ=255)                           tacggcctacggccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTc                                                                                                                         <  1:189349/149‑1 (MQ=255)                             cggcctacggccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCg                                                                                                                        <  5:150037/148‑1 (MQ=255)                              ggcctacggccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCgg                                                                                                                       >  7:35396/1‑148 (MQ=255)                               gcctacggccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCa                                                                                                                     >  6:351854/1‑149 (MQ=255)                                 ctacggccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTGCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCAcc                                                                                                                   <  8:238623/149‑1 (MQ=255)                                     ggccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCcaca                                                                                                               >  1:39846/1‑149 (MQ=255)                                      gccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCacac                                                                                                              >  3:177127/1‑149 (MQ=255)                                      gccAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCacac                                                                                                              <  4:140278/149‑1 (MQ=255)                                          gACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCCGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTcc                                                                                                          <  3:82001/149‑1 (MQ=255)                                                 gCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGTGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGt                                                                                                   <  5:282215/149‑1 (MQ=255)                                                  ccGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGTGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTa                                                                                                  <  6:37997/149‑1 (MQ=255)                                                  ccGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCg                                                                                                    >  5:119093/1‑147 (MQ=255)                                                  ccGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTa                                                                                                  <  2:371587/149‑1 (MQ=255)                                                   cGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAg                                                                                                 <  5:358396/149‑1 (MQ=255)                                                   cGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAg                                                                                                 <  8:86203/149‑1 (MQ=255)                                                   cGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAg                                                                                                 >  2:342104/1‑149 (MQ=255)                                                     ttGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGATGCGCCAGCGAGCCTGCATGAGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGt                                                                                               <  5:267553/149‑1 (MQ=255)                                                          cGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCa                                                                                          <  8:282700/149‑1 (MQ=255)                                                                cACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCAc                                                                                    >  2:308869/1‑149 (MQ=255)                                                                            cTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGt                                                                        <  6:119093/149‑1 (MQ=255)                                                                             ttGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCAc                                                                                         <  2:87486/131‑1 (MQ=255)                                                                             ttGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTc                                                                       >  2:193912/1‑149 (MQ=255)                                                                               gCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGc                                                                     >  3:94268/1‑149 (MQ=255)                                                                                   gTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCCCCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGt                                                                 >  3:223401/1‑149 (MQ=255)                                                                                        cGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGca                                                              >  7:100219/1‑147 (MQ=255)                                                                                           gTTGCGCCAGGGAGCCTGCATGAGGCGCGCCTTGAAGTCACCACAGTTGGAGCACGAGCTGAGTTCGCGGTAGTTGTCCTGGCTCGGCAACCACACTTCAAGATCGTAGGTGTTCACAGCCGAAAAGCCCATGTCGCCAGTGcagcggg                                                         >  5:65726/1‑144 (MQ=255)                                                                                           gTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTc                                                                                                           >  8:172909/1‑99 (MQ=255)                                                                                           gTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTc                                                                                                           <  7:172909/99‑1 (MQ=255)                                                                                           gTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAggg                                                         >  6:348577/1‑149 (MQ=255)                                                                                               cgcCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCa                                                      <  1:119539/148‑1 (MQ=255)                                                                                                      gAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTAGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTACAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCCCCACTGCACAGGGCCAGCAcgccg                                              >  2:325749/1‑147 (MQ=255)                                                                                                       aGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGt                                             <  1:308869/149‑1 (MQ=255)                                                                                                          cTGCATGCGGCGCGCCGGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCTCACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATg                                          >  1:120249/1‑149 (MQ=255)                                                                                                            gCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAACCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATgg                                         >  6:54617/1‑148 (MQ=255)                                                                                                             cATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATgg                                         <  5:351854/147‑1 (MQ=255)                                                                                                                 cggcgCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCCGCTc                                   >  4:231813/1‑149 (MQ=255)                                                                                                                 cggcgCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTc                                   <  1:342104/149‑1 (MQ=255)                                                                                                                         cTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCt                           <  4:177127/149‑1 (MQ=255)                                                                                                                         cTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCt                           <  5:148437/149‑1 (MQ=255)                                                                                                                           ggAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTc                                   >  7:110426/1‑139 (MQ=255)                                                                                                                           ggAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTc                                   <  8:110426/139‑1 (MQ=255)                                                                                                                                   ccacAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCAcgcg                 >  5:303671/1‑149 (MQ=255)                                                                                                                                      caGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTc              <  5:348577/149‑1 (MQ=255)                                                                                                                                       aGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCg             <  4:223401/149‑1 (MQ=255)                                                                                                                                       aGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCg             <  3:89203/149‑1 (MQ=255)                                                                                                                                              gCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTgg      <  8:100219/149‑1 (MQ=255)                                                                                                                                               cACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATgg                                         >  7:310371/1‑113 (MQ=255)                                                                                                                                               cACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATgg                                         <  8:310371/113‑1 (MQ=255)                                                                                                                                               cACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGc     <  4:155349/149‑1 (MQ=255)                                                                                                                                                  gAGCTGATTTAGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGt  <  4:94268/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GTAGTTTTCCAGCACAGCAACCAGGGTACGGCCTACGGCCAGACCGGAGCCGTTGAGCGTGTGCACCAGCTCTGGCTTGCCGGTTTCCGGGTTGCGCCAGCGAGCCTGCATGCGGCGCGCCTGGAAGTCACCACAGTTGGAGCACGAGCTGATTTCGCGGTACTTGTCCTGGCTCGGCACCCACACTTCCAGATCGTAGGTTTTCACCGCACCAAAGCCCATGTCGCCAGTGCACAGGGCCAGCACGCGGTATGGCAGCTCCAGCAGCTGCAGCACGCGCTCGGCATTGGCAGT  >  NC_002947/4508856‑4509149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑