Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 375,860 A→G 7.8% I384V (ATT→GTT)  PP_0312 → electron transfer flavoprotein subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947375,8600AG 7.8% 141.7 / 2.7 51I384V (ATT→GTT) PP_0312electron transfer flavoprotein subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (28/19);  new base G (2/2);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTGGTGCCCACCCCAGCTCCGG  >  NC_002947/375715‑376006
                                                                                                                                                  |                                                                                                                                                  
tACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTg                                                                                                                                                 <  5:361298/149‑1 (MQ=255)
   cTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTTACCTGTCGGTGAtt                                                                                                                                                  <  2:294977/145‑1 (MQ=255)
   cTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTAACCTGTCGGTGAtt                                                                                                                                                  >  1:294977/1‑145 (MQ=255)
                    tCTATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATtctc                                                                                                                             <  8:164165/149‑1 (MQ=255)
                      tATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATtc                                                                                                                               >  4:53596/1‑145 (MQ=255)
                      tATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATtc                                                                                                                               <  3:53596/145‑1 (MQ=255)
                         gtgGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGc                                                                                                                                               >  8:335095/1‑126 (MQ=255)
                         gtgGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGc                                                                                                                                               <  7:335095/126‑1 (MQ=255)
                           ggCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGGTTGGCGACAGTTCGGCGATTCTCAAGgcgc                                                                                                                      >  7:135750/1‑149 (MQ=255)
                           ggCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGGTTGGCGACAGTTCGGCGATTCTCAAGgcgc                                                                                                                      >  5:42775/1‑149 (MQ=255)
                            gCTGTTGGTATCTCGTGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCt                                                                                                                     <  8:149587/149‑1 (MQ=255)
                               gtgGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGGCCTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGa                                                                                                                   <  1:273297/149‑1 (MQ=255)
                                tgGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATc                                                                                                                 >  5:287655/1‑149 (MQ=255)
                                   gTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTTGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAg                                                                                                              >  5:265203/1‑149 (MQ=255)
                                   gTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAg                                                                                                              >  8:28171/1‑149 (MQ=255)
                                      tctcGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCt                                                                                                           >  4:124284/1‑149 (MQ=255)
                                        tcGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCtgt                                                                                                         >  3:293979/1‑149 (MQ=255)
                                                         ccTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCg                                                                                        >  3:238557/1‑149 (MQ=255)
                                                            gCAGGGTATCGGTGCCTGTGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCgg                                                                                       >  4:138454/1‑147 (MQ=255)
                                                              aGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGGTTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGcagc                                                                                   <  8:135750/149‑1 (MQ=255)
                                                              aGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGGTTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGcagc                                                                                   <  6:42775/149‑1 (MQ=255)
                                                              aGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGcagc                                                                                   >  7:131529/1‑149 (MQ=255)
                                                                          ccTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGAcgcg                                                                       >  3:333872/1‑149 (MQ=255)
                                                                             gCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCa                                                                    >  5:53246/1‑149 (MQ=255)
                                                                              cGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGc                                                                     <  1:379980/147‑1 (MQ=255)
                                                                              cGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGc                                                                     <  7:28171/147‑1 (MQ=255)
                                                                                  aaGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAgg                                                               >  3:368490/1‑149 (MQ=255)
                                                                                  aaGGTGGTGGAGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTTTCGGTGATTGGCGACAGTTCGTCGATTCTCAAGGCGCTGATCGAGGCTTTGGACAACTACCGCAGCGGCAGCCAGCGCGACTCGTCATAAgg                                                               <  4:188288/149‑1 (MQ=255)
                                                                                    ggtggtGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAggg                                                              >  2:93313/1‑148 (MQ=255)
                                                                                      tggtggCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCAc                                                           >  1:290302/1‑149 (MQ=255)
                                                                                      tggtggCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCAc                                                           <  6:287655/149‑1 (MQ=255)
                                                                                             gATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATg                                                    <  4:333872/149‑1 (MQ=255)
                                                                                               tCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAg                                                  >  3:218647/1‑149 (MQ=255)
                                                                                                 aaCATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTa                                                <  3:124284/149‑1 (MQ=255)
                                                                                                  aCATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTAc                                               >  6:279037/1‑149 (MQ=255)
                                                                                                         tCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGt                                         >  5:47590/1‑148 (MQ=255)
                                                                                                               cTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGc                                  >  3:158116/1‑149 (MQ=255)
                                                                                                                  cGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTg                               >  6:11471/1‑149 (MQ=255)
                                                                                                                        gATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATaa                                                                 <  7:127314/109‑1 (MQ=255)
                                                                                                                        gATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATaa                                                                 >  8:127314/1‑109 (MQ=255)
                                                                                                                         aTCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCa                        <  4:238557/149‑1 (MQ=255)
                                                                                                                            aaaCGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCAtt                      <  4:158116/148‑1 (MQ=255)
                                                                                                                            aaaCGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTg                     <  6:47590/149‑1 (MQ=255)
                                                                                                                            aaaCGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTg                     >  5:124712/1‑149 (MQ=255)
                                                                                                                            aaaCGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTg                     >  1:356118/1‑149 (MQ=255)
                                                                                                                             aaCGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTg                     <  3:316875/148‑1 (MQ=255)
                                                                                                                             aaCGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTg                     >  4:316875/1‑148 (MQ=255)
                                                                                                                                       aCCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTGGTGCCCAccc           <  3:138454/149‑1 (MQ=255)
                                                                                                                                         cTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTGGTGCCCAccc           >  1:66305/1‑147 (MQ=255)
                                                                                                                                              ggTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTGGTGCCCACCCCAGCTcc    >  1:170162/1‑149 (MQ=255)
                                                                                                                                                tGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTGGTGCCCACCCCAGCTCCgg  >  7:264924/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATCCAGCACCTGCAGGGTATCGGTGCCTGCGACAAGGTGGTGGCGATCAACATGGATCCGGGCTGCGACATGATCAAACGGG‑CTGACCTGTCGGTGATTGGCGACAGTTCGGCGATTCTCAAGGCGCTGATCGAGGCTGTGGACAACTACCGCAGCGGCAGCCAGCGCGACGCGGCATAAGGGCACGAGCATGAGTACGAAAGTGATCAGCCTGGTTTCCATTGGTGCCCACCCCAGCTCCGG  >  NC_002947/375715‑376006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑