| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,530,025 | T→A | 10.3% | intergenic (+31/‑137) | PP_4018 → / → topB | acetyltransferase/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,530,025 | 0 | T | A | 10.3% | 115.9 / 2.0 | 45 | intergenic (+31/‑137) | PP_4018/topB | acetyltransferase/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base T (27/13); new base A (3/2); total (30/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATG > NC_002947/4529889‑4530164 | gCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGGCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGGCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTAAACCCGCGAaga < 7:259089/149‑1 (MQ=255) cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAaga < 4:119521/148‑1 (MQ=255) cacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCgg > 8:3565/1‑149 (MQ=255) ttCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCCGGTTTACCAGCGAAGAGGCCGGCTCAGGc > 7:237711/1‑149 (MQ=255) gctaggggCGGCTGGGCTTTCGCGTTGGCCTGGTGGAATTCCTCGATGGTGGCATCCGCCATGTCTCCATGGTGCGCG‑ACGCGCGCGCCTGATCCATCACC‑GGCAGGGGCCTCTTCGCGGTTTTCCCCGCTATTAGGCCGGCTCAGGc < 2:303560/141‑1 (MQ=255) gggCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCg > 2:25862/1‑149 (MQ=255) tttCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGGTTTACCGCGAAGAGGGCGGCTCAGGCGAATTACATCCCCGcgg > 1:169367/1‑146 (MQ=255) cgTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGGGAAGAGGCCGGCTCAGGCGATATACATACCCGCTGACaaa > 8:79803/1‑148 (MQ=255) cgTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTAGCGGGGTTAACCGCGAAGAGGCCGGCTCAGGCGCTATACATCCCCGCTGACaaa > 1:172869/1‑148 (MQ=255) gTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTAGCGGGGTTACCCGCGAAGAGGCCGGCTCAGGCGATATAAATCCACGCTGACaaaa > 5:27829/1‑148 (MQ=255) tAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGAAGACGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACaaaa > 1:293231/1‑148 (MQ=255) agcttcGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGAAATACATCCCCGCTGACAAAAAAcc > 7:139314/6‑149 (MQ=255) gccgCGAGTAATTCCTGGAAGCTGGCAGCCCGCATGGCGACTTGGGGCGCG‑ACTGGCGCGCCTTATCCATCCCC‑TCCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCt < 6:163245/146‑1 (MQ=255) aGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTg > 1:90896/1‑148 (MQ=255) aGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcat > 5:221431/1‑149 (MQ=255) gaaTCCCGAATGTCGAATTGGTGGGGG‑CCTCGGGCGCCTTATCCATCACC‑TGCACGGGCATCTGGGGGTTTTTACCCGCTAAGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACtt < 4:317757/146‑1 (MQ=255) cGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACCGTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCctct > 1:268700/1‑149 (MQ=255) gCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTgc > 5:314152/1‑149 (MQ=255) aCATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGc < 7:3565/149‑1 (MQ=255) aCATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCATCGCGGGTTTACCCGCGAAGACGCCGGCACAGGCGATATAAAACCCCCCTTACAAAAAACCAGTTCATCCATCCAGATAAAACAAGCCTATGCGCCCCCGc > 4:126747/1‑149 (MQ=255) g‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCCCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgcgatgcag > 3:239468/1‑143 (MQ=255) cacggccgcgcgTCATCCATCCCC‑TGCACTGCCCTCTGCGGGGGTTTCCCGGCTAATTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCt < 6:27829/143‑1 (MQ=255) cgcgcgCCTTACCCAACACC‑TGCCCGGGCCTGTTCGCGGTTTTCCCCGCTACGCGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATgg < 8:139314/149‑1 (MQ=255) cgcgcgCCTGATCCATCACC‑TGCACTTGCCTCTTCGGGGTTTAACCGGGGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATgg < 1:25862/149‑1 (MQ=255) gcCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACTTCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCccac > 4:37589/1‑149 (MQ=255) cTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCccacca > 3:342335/1‑149 (MQ=255) ttcaaTCACG‑TGCACTGGCCTCTTCGCGGGTTTACCCGCTATGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAccc < 2:293231/145‑1 (MQ=255) aTCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAccc > 3:308687/1‑149 (MQ=255) aTCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGAt > 6:143828/1‑149 (MQ=255) aTCACC‑TGCACTGGCCTCTTAGCGGGTTTAACCGCGAAGATGCCCGCAAAGGCGATATACATACCCGCGGACAAAAAAACGGTACAAACATCCAGATAAAACATGGCTATGCGCCCCCCCTTGCGCAACCTATGGGCCACcccccccag > 4:207561/1‑146 (MQ=255) aCC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGaa < 4:308687/149‑1 (MQ=255) aCC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGaa < 2:90896/149‑1 (MQ=255) c‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAccc > 5:121668/1‑140 (MQ=255) c‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAccc < 6:121668/140‑1 (MQ=255) c‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGc > 6:305570/1‑149 (MQ=255) gCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCCTCCTATGGCCCACCCCCCCGATGAAGCGTCCCCgg > 7:103191/1‑149 (MQ=255) ctctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGa > 1:312905/1‑133 (MQ=255) ctctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGa < 2:312905/133‑1 (MQ=255) tctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCa > 5:213824/1‑149 (MQ=255) tctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCa > 8:76912/1‑149 (MQ=255) tctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCAt > 4:370843/1‑149 (MQ=255) tctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTTACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCa > 1:275871/1‑149 (MQ=255) ttCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATg > 1:251403/1‑149 (MQ=255) cgGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGcc > 6:267852/1‑142 (MQ=255) cgGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGcc < 5:267852/142‑1 (MQ=255) | GCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCG‑ACTCGCGCGCCTGATCCATCACC‑TGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATG > NC_002947/4529889‑4530164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |