Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,547,143	A→T	7.5%	L68H (CTC→CAC)	pydP ←	NCS1 family transporter PydP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,547,143	0	A	T	7.5%	196.0 / 4.9	67	L68H (CTC→CAC)	pydP	NCS1 family transporter PydP
Reads supporting (aligned to +/- strand):  ref base A (34/28);  new base T (3/2);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CCGAACGACGAACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATC  >  NC_002947/4546996‑4547282                                                                                                                                                    |                                                                                                                                                ccGAACGACGAACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAg                                                                                                                                                >  7:277974/1‑149 (MQ=255)  cGAACGACGAACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGc                                                                                                                                               >  6:298360/1‑149 (MQ=255)    aaCGACGAACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCAc                                                                                                                                             <  8:277974/149‑1 (MQ=255)     acgacgAACGCATCAGCACCGGAAACGGGATGACGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCTCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCAc                                                                                                                                             <  3:285329/148‑1 (MQ=255)         cgaACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCAcgccgc                                                                                                                                        >  4:146504/1‑149 (MQ=255)          gaACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACgccgcc                                                                                                                                       >  7:285775/1‑149 (MQ=255)             cGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAg                                                                                                                                    >  3:107695/1‑149 (MQ=255)                   agcaCCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAAGGCTTCGCCAACGCTGAGGACGAAGTAGGCGCTGAGCACGCCGCCGAGGgtg                                                                                                                                >  1:310847/1‑147 (MQ=255)                    gcaCCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAg                                                                                                                             <  7:312734/149‑1 (MQ=255)                       ccGGAAACGGGATGAAGTACTTGGTTCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGAACAATTACGTAGGCCAGCAAAATCGACAGCAGCGCTTCGCCAACGATCACGCCGAAGTAGGCGGTGAGCACGACGCAGAGGGTGTAGGTg                                                                                                                          >  4:287790/1‑149 (MQ=255)                        cGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTgg                                                                                                                         <  5:98576/149‑1 (MQ=255)                        cGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTgg                                                                                                                         <  4:122560/149‑1 (MQ=255)                                      cGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGgtgt                                                                                                                               >  4:64967/1‑129 (MQ=255)                                      cGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGgtgt                                                                                                                               <  3:64967/129‑1 (MQ=255)                                             ggTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑cc                                                                                                 <  1:149604/148‑1 (MQ=255)                                                gCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑ccacc                                                                                              >  8:139682/1‑148 (MQ=255)                                                 ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAgg                                                                                                                            >  2:72730/1‑121 (MQ=255)                                                 ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAgg                                                                                                                            <  1:72730/121‑1 (MQ=255)                                                 ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑ccaccca                                                                                            >  5:266265/1‑149 (MQ=255)                                                 ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑ccaccca                                                                                            >  3:226422/1‑149 (MQ=255)                                                      aaaGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAggg                                                                                       <  3:146504/149‑1 (MQ=255)                                                      aaaGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAggg                                                                                       <  6:255341/149‑1 (MQ=255)                                                        aGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCCAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCg                                                                                     >  1:126295/1‑149 (MQ=255)                                                         ggCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCgg                                                                                    >  6:23752/1‑149 (MQ=255)                                                             ttGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGa                                                                                 <  2:126295/148‑1 (MQ=255)                                                             ttGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGAt                                                                                <  5:250395/149‑1 (MQ=255)                                                             ttGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGAACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGAt                                                                                >  2:278667/1‑149 (MQ=255)                                                                      aGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGATGCCGAAGTAGGCGGTGAGCACGCCGCCGAGCGTGTAGGTGGGTACGCAG‑AAGGACATGC‑‑‑CAACCCACAGGGCGGTGATGTGCCAttt                                                                       >  3:201543/1‑149 (MQ=255)                                                                          gTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTc                                                                   >  1:29648/1‑149 (MQ=255)                                                                               aGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGt                                                              <  7:139682/149‑1 (MQ=255)                                                                                 cACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgc                                                            >  3:146248/1‑149 (MQ=255)                                                                                 cACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCAATTGTTCCAGGTgc                                                            >  8:178381/1‑149 (MQ=255)                                                                                  aCGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAGCATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgc                                                            <  1:278667/149‑1 (MQ=255)                                                                                  aCGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgcg                                                           >  7:320874/1‑149 (MQ=255)                                                                                  aCGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgcg                                                           >  1:46479/1‑149 (MQ=255)                                                                                   cGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgcgc                                                          >  1:289110/1‑149 (MQ=255)                                                                                      ttACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTgg                                                       <  6:266265/149‑1 (MQ=255)                                                                                          gTTGGCCAGCTAAATCGCCAGCAGCTCTTCGCCAACGCTGAGGCCGAAGTAGTCGGTGAGCACGCCGCCGAGTGTGTATGTGGGTACGCAG‑ATGGACATGC‑‑‑GCAGCCACAGGGCTGTGATTTGCCATTTGTTCCAGGTGCGCTGGTGCa                                                   <  4:201543/149‑1 (MQ=255)                                                                                          gTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCa                                                   >  4:96003/1‑149 (MQ=255)                                                                                              gCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCtt                                               <  1:178654/149‑1 (MQ=255)                                                                                                cAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTgg                                             >  6:166954/1‑149 (MQ=255)                                                                                                 aGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGCTGGGGGCCCACAGGGCGGTGATGTGCAATTTGTTCCAGGTGCGATGGTGCACCtt                                               >  1:312025/1‑149 (MQ=255)                                                                                                 aGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGt                                            >  5:68538/1‑149 (MQ=255)                                                                                                 aGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCACCAGCGCACCtggaa                                            <  8:285775/149‑5 (MQ=255)                                                                                                 aGCAAAAACGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGACCACGACGCCGAGGCTGTAGGTGGGTACGCAG‑ATGGACATGA‑‑‑CCACCCACAGGCCGCTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTAGGt                                            >  4:94140/1‑149 (MQ=255)                                                                                                  gCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTc                                           <  5:255827/149‑1 (MQ=255)                                                                                                  gCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTc                                           >  6:255827/1‑149 (MQ=255)                                                                                                     aaaTCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGt                                        <  5:166954/149‑1 (MQ=255)                                                                                                        tCGCCAGCAGCGCTTCGCCGACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTcc                                                                  >  5:16124/1‑120 (MQ=255)                                                                                                        tCGCCAGCAGCGCTTCGCCGACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTcc                                                                  <  6:16124/120‑1 (MQ=255)                                                                                                              gcagcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTg                                                        >  8:93046/1‑124 (MQ=255)                                                                                                              gcagcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTg                                                        <  7:93046/124‑1 (MQ=255)                                                                                                              gcagcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCg                               >  5:153496/1‑149 (MQ=255)                                                                                                                agcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCggg                             <  2:46479/149‑1 (MQ=255)                                                                                                                 gcgcTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCgggg                            >  3:281956/1‑149 (MQ=255)                                                                                                                       cGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTa                       <  3:96003/148‑1 (MQ=255)                                                                                                                         caaaCGCTGAGGCCGAAGTAGGCGGTGAGAACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCAAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTGGTAgcg                    >  6:127537/3‑149 (MQ=255)                                                                                                                               cTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCt              <  4:107695/149‑1 (MQ=255)                                                                                                                               cTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCt              <  2:289110/149‑1 (MQ=255)                                                                                                                                    gCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCAATTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGa         <  7:178381/149‑1 (MQ=255)                                                                                                                                     ccGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAg        >  1:241289/1‑149 (MQ=255)                                                                                                                                        ccctcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGa      >  4:279349/6‑148 (MQ=255)                                                                                                                                        aaGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGAc     <  4:111001/149‑1 (MQ=255)                                                                                                                                           tcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGt                                            <  1:300603/105‑1 (MQ=255)                                                                                                                                           tcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGt                                            >  2:300603/3‑107 (MQ=255)                                                                                                                                           tcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATc  >  8:327475/3‑149 (MQ=255)                                                                                                                                             ggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATc  <  7:327475/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                CCGAACGACGAACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATC  >  NC_002947/4546996‑4547282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑