Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,547,148	G→A	8.2%	G66G (GGC→GGT)	pydP ←	NCS1 family transporter PydP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,547,148	0	G	A	8.2%	170.9 / 4.7	61	G66G (GGC→GGT)	pydP	NCS1 family transporter PydP
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base A (3/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

CGAACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATCGCTGCCCTCGGACA  >  NC_002947/4547004‑4547296                                                                                                                                                 |                                                                                                                                                         cgaACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCAcgccgc                                                                                                                                                      >  4:146504/1‑149 (MQ=255)  gaACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACgccgcc                                                                                                                                                     >  7:285775/1‑149 (MQ=255)     cGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAg                                                                                                                                                  >  3:107695/1‑149 (MQ=255)           agcaCCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAAGGCTTCGCCAACGCTGAGGACGAAGTAGGCGCTGAGCACGCCGCCGAGGgtg                                                                                                                                              >  1:310847/1‑147 (MQ=255)            gcaCCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAg                                                                                                                                           <  7:312734/149‑1 (MQ=255)               ccGGAAACGGGATGAAGTACTTGGTTCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGAACAATTACGTAGGCCAGCAAAATCGACAGCAGCGCTTCGCCAACGATCACGCCGAAGTAGGCGGTGAGCACGACGCAGAGGGTGTAGGTg                                                                                                                                        >  4:287790/1‑149 (MQ=255)                cGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTgg                                                                                                                                       <  5:98576/149‑1 (MQ=255)                cGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTgg                                                                                                                                       <  4:122560/149‑1 (MQ=255)                              cGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGgtgt                                                                                                                                             >  4:64967/1‑129 (MQ=255)                              cGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGgtgt                                                                                                                                             <  3:64967/129‑1 (MQ=255)                                     ggTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑cc                                                                                                               <  1:149604/148‑1 (MQ=255)                                        gCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑ccacc                                                                                                            >  8:139682/1‑148 (MQ=255)                                         ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAgg                                                                                                                                          <  1:72730/121‑1 (MQ=255)                                         ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAgg                                                                                                                                          >  2:72730/1‑121 (MQ=255)                                         ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑ccaccca                                                                                                          >  5:266265/1‑149 (MQ=255)                                         ccGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGc‑‑‑ccaccca                                                                                                          >  3:226422/1‑149 (MQ=255)                                              aaaGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAggg                                                                                                     <  6:255341/149‑1 (MQ=255)                                              aaaGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAggg                                                                                                     <  3:146504/149‑1 (MQ=255)                                                aGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCCAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCg                                                                                                   >  1:126295/1‑149 (MQ=255)                                                 ggCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCgg                                                                                                  >  6:23752/1‑149 (MQ=255)                                                     ttGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGa                                                                                               <  2:126295/148‑1 (MQ=255)                                                     ttGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGAt                                                                                              <  5:250395/149‑1 (MQ=255)                                                     ttGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGAACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGAt                                                                                              >  2:278667/1‑149 (MQ=255)                                                              aGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGATGCCGAAGTAGGCGGTGAGCACGCCGCCGAGCGTGTAGGTGGGTACGCAG‑AAGGACATGC‑‑‑CAACCCACAGGGCGGTGATGTGCCAttt                                                                                     >  3:201543/1‑149 (MQ=255)                                                                  gTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTc                                                                                 >  1:29648/1‑149 (MQ=255)                                                                       aGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGt                                                                            <  7:139682/149‑1 (MQ=255)                                                                         cACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgc                                                                          >  3:146248/1‑149 (MQ=255)                                                                         cACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCAATTGTTCCAGGTgc                                                                          >  8:178381/1‑149 (MQ=255)                                                                          aCGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAGCATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgc                                                                          <  1:278667/149‑1 (MQ=255)                                                                          aCGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgcg                                                                         >  7:320874/1‑149 (MQ=255)                                                                          aCGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgcg                                                                         >  1:46479/1‑149 (MQ=255)                                                                           cGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTgcgc                                                                        >  1:289110/1‑149 (MQ=255)                                                                              ttACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTgg                                                                     <  6:266265/149‑1 (MQ=255)                                                                                  gTTGGCCAGCTAAATCGCCAGCAGCTCTTCGCCAACGCTGAGGCCGAAGTAGTCGGTGAGCACGCCGCCGAGTGTGTATGTGGGTACGCAG‑ATGGACATGC‑‑‑GCAGCCACAGGGCTGTGATTTGCCATTTGTTCCAGGTGCGCTGGTGCa                                                                 <  4:201543/149‑1 (MQ=255)                                                                                  gTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCa                                                                 >  4:96003/1‑149 (MQ=255)                                                                                      gCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCtt                                                             <  1:178654/149‑1 (MQ=255)                                                                                        cAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTgg                                                           >  6:166954/1‑149 (MQ=255)                                                                                         aGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGCTGGGGGCCCACAGGGCGGTGATGTGCAATTTGTTCCAGGTGCGATGGTGCACCtt                                                             >  1:312025/1‑149 (MQ=255)                                                                                         aGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGt                                                          >  5:68538/1‑149 (MQ=255)                                                                                         aGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCACCAGCGCACCtggaa                                                          <  8:285775/149‑5 (MQ=255)                                                                                         aGCAAAAACGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGACCACGACGCCGAGGCTGTAGGTGGGTACGCAG‑ATGGACATGA‑‑‑CCACCCACAGGCCGCTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTAGGt                                                          >  4:94140/1‑149 (MQ=255)                                                                                          gCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTc                                                         >  6:255827/1‑149 (MQ=255)                                                                                          gCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTc                                                         <  5:255827/149‑1 (MQ=255)                                                                                             aaaTCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGt                                                      <  5:166954/149‑1 (MQ=255)                                                                                                tCGCCAGCAGCGCTTCGCCGACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTcc                                                                                >  5:16124/1‑120 (MQ=255)                                                                                                tCGCCAGCAGCGCTTCGCCGACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTcc                                                                                <  6:16124/120‑1 (MQ=255)                                                                                                      gcagcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTg                                                                      >  8:93046/1‑124 (MQ=255)                                                                                                      gcagcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTg                                                                      <  7:93046/124‑1 (MQ=255)                                                                                                      gcagcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCg                                             >  5:153496/1‑149 (MQ=255)                                                                                                        agcGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCggg                                           <  2:46479/149‑1 (MQ=255)                                                                                                         gcgcTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCgggg                                          >  3:281956/1‑149 (MQ=255)                                                                                                               cGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTa                                     <  3:96003/148‑1 (MQ=255)                                                                                                                 caaaCGCTGAGGCCGAAGTAGGCGGTGAGAACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCAAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTGGTAgcg                                  >  6:127537/3‑149 (MQ=255)                                                                                                                       cTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCt                            <  4:107695/149‑1 (MQ=255)                                                                                                                       cTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCt                            <  2:289110/149‑1 (MQ=255)                                                                                                                            gCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCAATTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGa                       <  7:178381/149‑1 (MQ=255)                                                                                                                             ccGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAg                      >  1:241289/1‑149 (MQ=255)                                                                                                                                ccctcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGa                    >  4:279349/6‑148 (MQ=255)                                                                                                                                aaGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGAc                   <  4:111001/149‑1 (MQ=255)                                                                                                                                   tcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGt                                                          <  1:300603/105‑1 (MQ=255)                                                                                                                                   tcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGt                                                          >  2:300603/3‑107 (MQ=255)                                                                                                                                   tcggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATc                >  8:327475/3‑149 (MQ=255)                                                                                                                                     ggcggcGTGCTCACCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATc                <  7:327475/147‑1 (MQ=255)                                                                                                                                                 gccgccGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATCGCTGCCCTCGGACa  <  6:68538/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                         CGAACGCAGCAGCACCGGAAACGGGATGCCGTACTTGGTGCCGGGAAAGGCATTGAGGGTAAGCGGTATCAGCACGATTACGTTGGCCAGCAAAATCGCCAGCAGCGCTTCGCCAACGCTGAGGCCGAAGTAGGCGGTGAGCACGCCGCCGAGGGTGTAGGTGGGTACGCAG‑ATGGACATGC‑‑‑CCACCCACAGGGCGGTGATGTGCCATTTGTTCCAGGTGCGCTGGTGCACCTTGGTCGGTGCTATGTCGGGGTTGTAGCGCGGGCTGTCGAGGACATCGCTGCCCTCGGACA  >  NC_002947/4547004‑4547296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑