Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,584,069 C→T 8.3% L505L (TTG→TTA)  glgE ← alpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,584,0690CT 8.3% 136.6 / 2.3 48L505L (TTG→TTA) glgEalpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base T (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

GGTTGAGCTGGGCGATCTCGGCGATGATGTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACTGGCTCCACGGCGGCTGATTGAGCTGC  >  NC_002947/4583924‑4584213
                                                                                                                                                 |                                                                                                                                                 
ggTTGAGCTGGGCGATCTCGGCGATGATGTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAg                                                                                                                                                >  4:325653/1‑149 (MQ=255)
       cTGGGCGATCTCGGCGATGATGTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGc                                                                                                                                         <  6:234510/149‑1 (MQ=255)
       cTGGGCGATCTCGGCGATGATGTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCACCACAAGCCTGAGc                                                                                                                                         >  8:297133/1‑149 (MQ=255)
        tGGGCGATCTCGGCGATGATGTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGcc                                                                                                                                        >  3:360490/1‑149 (MQ=255)
                   ggCGATGATGTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGt                                                                                                                                  <  3:279402/144‑1 (MQ=255)
                         gatgTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCgg                                                                                                                       >  1:146328/1‑149 (MQ=255)
                          atgTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCgg                                                                                                                       <  5:312411/148‑1 (MQ=255)
                                 cGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATc                                                                                                               <  2:139742/149‑1 (MQ=255)
                                     cTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGa                                                                                                           <  3:325653/149‑1 (MQ=255)
                                        ggTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAAc                                                                                                        <  1:281640/149‑1 (MQ=255)
                                             aaTCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTgcg                                                                                                   <  2:146328/149‑1 (MQ=255)
                                                cgcgTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCgg                                                                                                >  4:1190/1‑149 (MQ=255)
                                                      ggCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAg                                                                                          <  6:310707/149‑1 (MQ=255)
                                                       gCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAgg                                                                                         >  1:201000/1‑149 (MQ=255)
                                                           aTTTCGTACTTCTCCGAATCCACGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGtgtg                                                                                     <  8:51941/149‑1 (MQ=255)
                                                              tCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCa                                                                                   <  8:290968/148‑1 (MQ=255)
                                                              tCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAg                                                                                  >  6:310223/1‑149 (MQ=255)
                                                                   cTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGaaa                                                                              >  8:39401/1‑148 (MQ=255)
                                                                    ttCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGaaaaa                                                                            <  5:281003/149‑1 (MQ=255)
                                                                        ccGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCa                                                                                                              <  6:255653/111‑1 (MQ=255)
                                                                        ccGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCa                                                                                                              >  5:255653/1‑111 (MQ=255)
                                                                         cGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAActg                                                                         <  5:284771/147‑3 (MQ=255)
                                                                         cGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACgg‑g                                                                       <  7:302217/148‑1 (MQ=255)
                                                                            aTCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGa                                                                   <  3:1190/149‑1 (MQ=255)
                                                                                aGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTc                                                               >  2:198730/1‑149 (MQ=255)
                                                                                       cctcctTGACCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGGACGGAACAGGAAACATGCGCGGCCTGAGGTGAGCACGAAAAACGG‑GTTGAGGTCCGGTGTa                                                        >  7:251886/1‑149 (MQ=255)
                                                                                           ctTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGa                                                    <  7:297133/149‑1 (MQ=255)
                                                                                                ccGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACg                                                  >  5:14798/1‑146 (MQ=255)
                                                                                                 cGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTGCGGTGTATTGACgaag                                               >  4:114807/1‑148 (MQ=255)
                                                                                                 cGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACgaag                                               >  4:109575/1‑148 (MQ=255)
                                                                                                  ggCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCATAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGgt                                                           <  4:363581/135‑1 (MQ=255)
                                                                                                  ggCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCATAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGgt                                                           <  4:357765/135‑1 (MQ=255)
                                                                                                  ggCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCATAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGgt                                                           >  3:363581/1‑135 (MQ=255)
                                                                                                  ggCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCATAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGgt                                                           >  3:357765/1‑135 (MQ=255)
                                                                                                                 tCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGGCGTAGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAg                                >  1:127177/1‑148 (MQ=255)
                                                                                                                 tCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGAACAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCa                              >  6:93006/1‑149 (MQ=255)
                                                                                                                   gCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCAc                             <  4:360490/148‑1 (MQ=255)
                                                                                                                   gCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACt                            >  3:118707/1‑149 (MQ=255)
                                                                                                                   gCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACt                            <  2:201000/149‑1 (MQ=255)
                                                                                                                     acaGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAg                                                                                          >  6:329243/1‑86 (MQ=255)
                                                                                                                     acaGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAg                                                                                          <  5:329243/86‑1 (MQ=255)
                                                                                                                      caGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGAACAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACTGGc                         <  5:93006/149‑1 (MQ=255)
                                                                                                                            gAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCgg                                   >  3:244013/1‑133 (MQ=255)
                                                                                                                            gAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCgg                                   <  4:244013/133‑1 (MQ=255)
                                                                                                                                         aTGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACTGGCTCCACGGCGGCTGATTGAg      <  6:14798/149‑1 (MQ=255)
                                                                                                                                         aTGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACTGGCTCCACGGCGGCTGATTGAg      >  4:330136/1‑149 (MQ=255)
                                                                                                                                         aTGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACTGGCTCCACGGCGGCTGATTGAg      >  4:250319/1‑149 (MQ=255)
                                                                                                                                             cccACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACTGGCTCCACGGCGGCTGATTGAgctgc  <  7:39401/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGTTGAGCTGGGCGATCTCGGCGATGATGTTGCCGGGCTGGGTGAAATCGCGTGGGCGGATTTCGTACTTCTCCGAATCCAGGTACTCCTCCTTGCCCGGCAACGGCGTGCCCTCGCACAGCTCGAAGCCCGAATACATGCCCCACAAGCCTGAGCCCATGGTCGCCAGCGCGGCACGGATCAGGAAACCTGCGCGGCCTGAGGTGTGCAGGAAAAACGG‑GTTGATGTCCGGTGTATTGACGAAGAAGTTGGGCCGGTAGCACTGGCTCCACGGCGGCTGATTGAGCTGC  >  NC_002947/4583924‑4584213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑