Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,632,595	G→C	7.8%	noncoding (35/76 nt)	PP_t59 ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,632,595	0	G	C	7.8%	191.3 / 7.1	64	noncoding (35/76 nt)	PP_t59	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (28/31);  new base C (3/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

GTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAG  >  NC_002947/4632448‑4632737                                                                                                                                                    |                                                                                                                                               gTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                <  5:31027/148‑1 (MQ=255)         cGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgg                                                                                                                                       <  6:114693/149‑2 (MQ=255)          ggggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                      <  3:290095/149‑1 (MQ=255)                ttGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                                                                                >  1:342213/1‑149 (MQ=255)                    ggCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATC‑TTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                           <  6:45086/149‑1 (MQ=255)                        caaTGCATTCCACCCAAAAACGTCCCCGAAGGGCCTTTTTTCAACGACTTACCGACTTCGACTGAAACCTGTAGTTCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                        <  8:296845/148‑1 (MQ=21)                            gCATTCGAAAGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAt                                                                                                                    <  2:298224/149‑1 (MQ=255)                                   aacgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgcg                                                                                                             >  4:232959/1‑149 (MQ=255)                                   aacgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgcg                                                                                                             >  1:121796/1‑149 (MQ=255)                                     tgaaAAAAGGCCCTTCGGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgtc                                                                                                           >  1:175103/2‑149 (MQ=255)                                         aaaaGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGa                                                                                                       <  5:20613/149‑1 (MQ=255)                                          aaaGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGAt                                                                                                      >  7:193/1‑149 (MQ=255)                                            aGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                    <  7:316346/149‑1 (MQ=255)                                            aGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                    <  8:6038/149‑1 (MQ=255)                                            aGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                    <  2:303116/149‑1 (MQ=255)                                              gCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGt                                                                                                  >  4:75319/1‑149 (MQ=255)                                                cccGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGc                                                                                                >  1:142229/1‑149 (MQ=255)                                                   gAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTTATGGGTGCCAt                                                                                             >  1:312970/1‑149 (MQ=255)                                                       ggCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCTAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgtc                                                                                                           <  6:206481/131‑1 (MQ=255)                                                       ggCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCTAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgtc                                                                                                           >  5:206481/1‑131 (MQ=255)                                                        gCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAg                                                                                        <  2:142229/149‑1 (MQ=255)                                                                tCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTc                                                                                 >  7:65713/1‑148 (MQ=255)                                                                  aaCGACTTACAGACTTCGACTGAAACCTGTAGAGCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTaa                                                                              <  6:61684/149‑1 (MQ=255)                                                                      aCTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACa                                                                          <  4:365286/149‑1 (MQ=255)                                                                        ttACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGc                                                                        >  2:237990/1‑149 (MQ=255)                                                                          aCAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACCGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGc                                                                        <  5:214886/147‑1 (MQ=255)                                                                           cAGACTTCGACTGAAAGCTGTCGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGt                                                                      <  2:248598/148‑1 (MQ=255)                                                                            aGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAg                                                                                        >  6:264695/1‑129 (MQ=255)                                                                            aGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAg                                                                                        <  5:264695/129‑1 (MQ=255)                                                                            aGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCCTGCTATACCAACTGAGCTATTCCCGCGTCTTGATGGGTGCCATTCTAGCGATTTCTAAAACACCGTc                                                                     >  7:345426/1‑148 (MQ=255)                                                                              aCTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAc                                                                  <  3:47586/149‑1 (MQ=255)                                                                               cTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAc                                                                  <  1:373388/148‑1 (MQ=255)                                                                                ttCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAccc                                                                >  7:205723/1‑149 (MQ=255)                                                                                ttCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAccc                                                                >  5:216208/1‑149 (MQ=255)                                                                                   gACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCtt                                                             >  7:66138/1‑149 (MQ=255)                                                                                   gACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCt                                                              <  3:232959/148‑1 (MQ=255)                                                                                    aCTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCtt                                                             >  8:344094/1‑148 (MQ=255)                                                                                        aaaCCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGAtt                                                         <  8:66138/148‑1 (MQ=255)                                                                                           ccTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCaaa                                                     <  1:237990/149‑1 (MQ=255)                                                                                              gTAGATCTTTATTTGGAGCGGGAAACGAGACTCTAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTaaaa                                                                            <  2:248957/123‑1 (MQ=255)                                                                                              gTAGATCTTTATTTGGAGCGGGAAAAGAGACTCGAACTCGCGAACCCGACCTTGGCAAGGTCGTGCTCTACCAACTTATCTATTCCCGCGTCGTTATTGGTGGCATTCTAGCGATTTCTaaaa                                                                            >  1:248957/1‑123 (MQ=18)                                                                                                     tttATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTAt                                           >  4:148439/1‑149 (MQ=255)                                                                                                      ttATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTAtt                                          >  6:120604/1‑149 (MQ=255)                                                                                                       tATTTGGAGCGGGAAACGAGACTCGAACTCGAGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACttt                                              <  2:312970/144‑1 (MQ=255)                                                                                                         tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTAttttt                                       <  8:205723/149‑1 (MQ=255)                                                                                                         tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTAttttt                                       <  2:195649/149‑1 (MQ=255)                                                                                                           tGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCGTTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTAtttttt                                      <  2:121796/148‑1 (MQ=255)                                                                                                            ggAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCACCCCCTTGATTCAAAAAACTTTTAtttttttt                                    >  6:343912/1‑149 (MQ=255)                                                                                                             gAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTc                                   >  3:193309/1‑149 (MQ=255)                                                                                                                cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGa                                <  6:216208/149‑1 (MQ=255)                                                                                                                cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGa                                <  8:193/149‑1 (MQ=255)                                                                                                                cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGa                                <  3:75319/149‑1 (MQ=255)                                                                                                                    aaaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACAAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACACCGTCAACCCATTTATTCAAAAAAATTTTATTTTTTTTCAGAGcat                            >  7:232374/1‑147 (MQ=25)                                                                                                                        gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTcgc                        >  4:196364/1‑149 (MQ=255)                                                                                                                           aCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTCAAACAGCGTCCACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGCGCCTTcgcg                       >  7:357032/1‑147 (MQ=255)                                                                                                                               gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAGCCCCGTGATTCAAAAAGCTTTTATTTTTTTTCCGAGCCTTCGCGAAGGTg                 >  4:84981/1‑149 (MQ=255)                                                                                                                                 aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcg               <  2:175103/149‑1 (MQ=255)                                                                                                                                      cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccag          <  5:343912/149‑1 (MQ=255)                                                                                                                                        aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggc        <  7:344094/149‑1 (MQ=255)                                                                                                                                          cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcgg      >  1:64827/1‑149 (MQ=255)                                                                                                                                          cacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCt                                                              <  6:255098/91‑1 (MQ=255)                                                                                                                                          cacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCt                                                              >  5:255098/3‑93 (MQ=255)                                                                                                                                          cacGACCTTGCCAAGGTCGGGGTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcgg      >  4:356450/3‑149 (MQ=255)                                                                                                                                           acGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcggc     >  3:287971/2‑149 (MQ=255)                                                                                                                                               ccTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcggccag  >  7:344256/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                               GTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAG  >  NC_002947/4632448‑4632737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑