Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,700,769 A→G 7.7% intergenic (‑82/+68) kdpD ← / ← kdpC sensor protein/potassium‑transporting ATPase subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,700,7690AG 7.7% 191.1 / 5.1 65intergenic (‑82/+68)kdpD/kdpCsensor protein/potassium‑transporting ATPase subunit C
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base G (3/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

AGCCGGCCCCGGCCGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGA  >  NC_002947/4700623‑4700916
                                                                                                                                                  |                                                                                                                                                   
aGCCGGCCCCGGCCGGTTCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATAc                                                                                                                                                    <  7:337968/148‑1 (MQ=255)
  ccggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                  >  7:269729/1‑148 (MQ=255)
  ccggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                  >  4:339160/1‑148 (MQ=255)
   cggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCa                                                                                                                                                <  8:152313/149‑1 (MQ=255)
    ggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAg                                                                                                                                               <  5:204276/149‑1 (MQ=255)
    ggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAg                                                                                                                                               <  5:209275/149‑1 (MQ=255)
        ccggccggGGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCCGGCGGAGTCACTCATGTGTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCCGGGAAATCCCCCATAACGTGTCGGAGCCGCTTAACCCGCGATTACGTCAGTgg                                                                                                                                            >  4:281388/1‑148 (MQ=255)
         cggccggTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGca                                                                                                                                          >  4:93985/1‑149 (MQ=255)
                        aGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTcg                                                                                                                           >  7:18555/1‑149 (MQ=255)
                           ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCgg                                                                                                                        <  6:17151/149‑1 (MQ=255)
                           ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCgg                                                                                                                        <  1:125622/149‑1 (MQ=255)
                           ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg                                                                                                                        <  1:209051/149‑1 (MQ=25)
                             ggCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGt                                                                                                                      >  7:201695/1‑149 (MQ=255)
                              gCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGCGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGt                                                                                                                      <  3:20359/148‑1 (MQ=255)
                               ccAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTg                                                                                                                     >  3:285947/1‑148 (MQ=255)
                                cAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGaa                                                                                                                   >  4:267954/1‑149 (MQ=255)
                                 aaCAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAAc                                                                                                                  >  1:366860/1‑149 (MQ=255)
                                  aCAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAAcc                                                                                                                 <  8:18555/149‑1 (MQ=255)
                                      cgcATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg                                                                                                                        <  5:29472/138‑1 (MQ=21)
                                      cgcATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg                                                                                                                        >  6:29472/1‑138 (MQ=21)
                                         aTCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc                                                                                                           >  3:257006/1‑148 (MQ=255)
                                             gcgcGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAg                                                                                                      <  7:354793/149‑1 (MQ=255)
                                                    cggAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCt                                                                                               >  6:36742/1‑149 (MQ=255)
                                                    cggAGTCACTCATGGTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCt                                                                                               <  7:129375/149‑1 (MQ=255)
                                                        gTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCa                                                                                           <  8:269729/149‑1 (MQ=255)
                                                           aCTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAgcg                                                                                        >  5:203465/1‑149 (MQ=255)
                                                             tCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTgg                                                                                                                                            <  5:5903/95‑1 (MQ=255)
                                                             tCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTgg                                                                                                                                            >  6:5903/1‑95 (MQ=255)
                                                                  tttCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGAt                                                                                 <  6:58223/149‑1 (MQ=255)
                                                                     cATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTAc                                                                              >  8:279219/1‑149 (MQ=255)
                                                                      aTCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                  >  5:290502/1‑80 (MQ=255)
                                                                      aTCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                  <  6:290502/80‑1 (MQ=255)
                                                                      aTCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTAc                                                                              <  6:338040/148‑1 (MQ=255)
                                                                         cTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGa                                                                          >  2:279213/1‑149 (MQ=255)
                                                                          tttACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGa                                                                          >  5:69442/1‑148 (MQ=255)
                                                                              cTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATg                                                                     >  8:192141/1‑149 (MQ=255)
                                                                                 tGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGc                                                                   <  6:203465/148‑1 (MQ=255)
                                                                                   tCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAg                                                                >  4:352598/1‑149 (MQ=255)
                                                                                     cAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAAt                                                                      <  3:310556/141‑1 (MQ=255)
                                                                                     cAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAAt                                                                      >  4:310556/1‑141 (MQ=255)
                                                                                      aaCAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCtt                                                             >  3:84012/1‑149 (MQ=255)
                                                                                              gccgcTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAgg                                                     >  7:230940/1‑149 (MQ=255)
                                                                                              gccgcTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAgg                                                     >  8:83998/1‑149 (MQ=255)
                                                                                                    tCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGt                                               >  6:141284/1‑149 (MQ=255)
                                                                                                         gAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAAc                                          <  4:84012/149‑1 (MQ=255)
                                                                                                             gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa                                      <  8:230940/149‑1 (MQ=255)
                                                                                                             gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa                                      <  7:279219/149‑1 (MQ=255)
                                                                                                             gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa                                      <  3:93985/149‑1 (MQ=255)
                                                                                                               ccccTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAg                                     >  6:76270/1‑148 (MQ=255)
                                                                                                                cccTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCa                                   >  6:251669/1‑149 (MQ=255)
                                                                                                                    tAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc                                                                                                           >  3:256707/1‑73 (MQ=255)
                                                                                                                    tAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc                                                                                                           <  4:256707/73‑1 (MQ=255)
                                                                                                                     aaCTTGTAGGTGGGGTTTTACCCGCGAATACGTCAGTGGCACCACCCCCGCATTCGCGGGTGAACCCGGTCCTACAGGCTTCCTGCCAGCTCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCTCGtt                               <  8:196495/148‑1 (MQ=38)
                                                                                                                      aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTg                              <  8:201695/148‑1 (MQ=255)
                                                                                                                      aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGa                             <  1:15943/149‑1 (MQ=255)
                                                                                                                      aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGa                             <  3:267954/149‑1 (MQ=255)
                                                                                                                             ggAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACcgg                      >  6:224697/1‑149 (MQ=255)
                                                                                                                              gAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTAGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACcggc                     >  6:60126/1‑149 (MQ=255)
                                                                                                                                   ggTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCGGGTAAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGcc                >  5:208515/1‑149 (MQ=21)
                                                                                                                                    gTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCGGGTAAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCg               >  2:366383/1‑149 (MQ=21)
                                                                                                                                     tttACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGa              >  2:60970/1‑149 (MQ=255)
                                                                                                                                       tACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGAt             <  7:192141/148‑1 (MQ=255)
                                                                                                                                              gAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGTCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGt     <  1:279213/149‑1 (MQ=255)
                                                                                                                                                aTACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTg    >  6:234927/1‑148 (MQ=255)
                                                                                                                                                 tACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGa  <  5:36742/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCCGGCCCCGGCCGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGA  >  NC_002947/4700623‑4700916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑