Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,700,772 T→G 8.2% intergenic (‑85/+65) kdpD ← / ← kdpC sensor protein/potassium‑transporting ATPase subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,700,7720TG 8.2% 185.8 / 7.8 61intergenic (‑85/+65)kdpD/kdpCsensor protein/potassium‑transporting ATPase subunit C
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base G (3/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

CCGGCCCCGGCCGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGAGGG  >  NC_002947/4700625‑4700919
                                                                                                                                                   |                                                                                                                                                   
ccggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                     >  4:339160/1‑148 (MQ=255)
ccggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                     >  7:269729/1‑148 (MQ=255)
 cggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCa                                                                                                                                                   <  8:152313/149‑1 (MQ=255)
  ggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAg                                                                                                                                                  <  5:209275/149‑1 (MQ=255)
  ggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAg                                                                                                                                                  <  5:204276/149‑1 (MQ=255)
      ccggccggGGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCCGGCGGAGTCACTCATGTGTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCCGGGAAATCCCCCATAACGTGTCGGAGCCGCTTAACCCGCGATTACGTCAGTgg                                                                                                                                               >  4:281388/1‑148 (MQ=255)
       cggccggTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGca                                                                                                                                             >  4:93985/1‑149 (MQ=255)
                      aGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTcg                                                                                                                              >  7:18555/1‑149 (MQ=255)
                         ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCgg                                                                                                                           <  1:125622/149‑1 (MQ=255)
                         ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCgg                                                                                                                           <  6:17151/149‑1 (MQ=255)
                         ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg                                                                                                                           <  1:209051/149‑1 (MQ=25)
                           ggCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGt                                                                                                                         >  7:201695/1‑149 (MQ=255)
                            gCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGCGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGt                                                                                                                         <  3:20359/148‑1 (MQ=255)
                             ccAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTg                                                                                                                        >  3:285947/1‑148 (MQ=255)
                              cAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGaa                                                                                                                      >  4:267954/1‑149 (MQ=255)
                               aaCAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAAc                                                                                                                     >  1:366860/1‑149 (MQ=255)
                                aCAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAAcc                                                                                                                    <  8:18555/149‑1 (MQ=255)
                                    cgcATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg                                                                                                                           >  6:29472/1‑138 (MQ=21)
                                    cgcATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg                                                                                                                           <  5:29472/138‑1 (MQ=21)
                                       aTCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc                                                                                                              >  3:257006/1‑148 (MQ=255)
                                           gcgcGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAg                                                                                                         <  7:354793/149‑1 (MQ=255)
                                                  cggAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCt                                                                                                  >  6:36742/1‑149 (MQ=255)
                                                  cggAGTCACTCATGGTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCt                                                                                                  <  7:129375/149‑1 (MQ=255)
                                                      gTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCa                                                                                              <  8:269729/149‑1 (MQ=255)
                                                         aCTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAgcg                                                                                           >  5:203465/1‑149 (MQ=255)
                                                           tCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTgg                                                                                                                                               >  6:5903/1‑95 (MQ=255)
                                                           tCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTgg                                                                                                                                               <  5:5903/95‑1 (MQ=255)
                                                                tttCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGAt                                                                                    <  6:58223/149‑1 (MQ=255)
                                                                   cATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTAc                                                                                 >  8:279219/1‑149 (MQ=255)
                                                                    aTCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                     <  6:290502/80‑1 (MQ=255)
                                                                    aTCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGt                                                                                                                                                     >  5:290502/1‑80 (MQ=255)
                                                                    aTCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTAc                                                                                 <  6:338040/148‑1 (MQ=255)
                                                                       cTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGa                                                                             >  2:279213/1‑149 (MQ=255)
                                                                        tttACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGa                                                                             >  5:69442/1‑148 (MQ=255)
                                                                            cTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATg                                                                        >  8:192141/1‑149 (MQ=255)
                                                                               tGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGc                                                                      <  6:203465/148‑1 (MQ=255)
                                                                                 tCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAg                                                                   >  4:352598/1‑149 (MQ=255)
                                                                                   cAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAAt                                                                         >  4:310556/1‑141 (MQ=255)
                                                                                   cAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAAt                                                                         <  3:310556/141‑1 (MQ=255)
                                                                                    aaCAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCtt                                                                >  3:84012/1‑149 (MQ=255)
                                                                                            gccgcTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAgg                                                        >  7:230940/1‑149 (MQ=255)
                                                                                            gccgcTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAgg                                                        >  8:83998/1‑149 (MQ=255)
                                                                                                  tCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGt                                                  >  6:141284/1‑149 (MQ=255)
                                                                                                       gAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAAc                                             <  4:84012/149‑1 (MQ=255)
                                                                                                           gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa                                         <  8:230940/149‑1 (MQ=255)
                                                                                                           gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa                                         <  7:279219/149‑1 (MQ=255)
                                                                                                           gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa                                         <  3:93985/149‑1 (MQ=255)
                                                                                                             ccccTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAg                                        >  6:76270/1‑148 (MQ=255)
                                                                                                              cccTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCa                                      >  6:251669/1‑149 (MQ=255)
                                                                                                                  tAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc                                                                                                              >  3:256707/1‑73 (MQ=255)
                                                                                                                  tAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc                                                                                                              <  4:256707/73‑1 (MQ=255)
                                                                                                                   aaCTTGTAGGTGGGGTTTTACCCGCGAATACGTCAGTGGCACCACCCCCGCATTCGCGGGTGAACCCGGTCCTACAGGCTTCCTGCCAGCTCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCTCGtt                                  <  8:196495/148‑1 (MQ=38)
                                                                                                                    aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTg                                 <  8:201695/148‑1 (MQ=255)
                                                                                                                    aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGa                                <  1:15943/149‑1 (MQ=255)
                                                                                                                    aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGa                                <  3:267954/149‑1 (MQ=255)
                                                                                                                           ggAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACcgg                         >  6:224697/1‑149 (MQ=255)
                                                                                                                            gAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTAGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACcggc                        >  6:60126/1‑149 (MQ=255)
                                                                                                                                 ggTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCGGGTAAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGcc                   >  5:208515/1‑149 (MQ=21)
                                                                                                                                  gTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCGGGTAAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCg                  >  2:366383/1‑149 (MQ=21)
                                                                                                                                   tttACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGa                 >  2:60970/1‑149 (MQ=255)
                                                                                                                                     tACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGAt                <  7:192141/148‑1 (MQ=255)
                                                                                                                                            gAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGTCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGt        <  1:279213/149‑1 (MQ=255)
                                                                                                                                              aTACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTg       >  6:234927/1‑148 (MQ=255)
                                                                                                                                               tACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGa     <  5:36742/149‑1 (MQ=255)
                                                                                                                                                  gTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGAggg  >  8:262184/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGCCCCGGCCGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGAGGG  >  NC_002947/4700625‑4700919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: