| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,700,774 | A→G | 8.3% | intergenic (‑87/+63) | kdpD ← / ← kdpC | sensor protein/potassium‑transporting ATPase subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,700,774 | 0 | A | G | 8.3% | 170.8 / 5.3 | 60 | intergenic (‑87/+63) | kdpD/kdpC | sensor protein/potassium‑transporting ATPase subunit C |
| Reads supporting (aligned to +/- strand): ref base A (29/26); new base G (3/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01 | |||||||||||
CGGCCCCGGCCGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGAGGG > NC_002947/4700626‑4700919 | cggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCa < 8:152313/149‑1 (MQ=255) ggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAg < 5:209275/149‑1 (MQ=255) ggccccggccGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAg < 5:204276/149‑1 (MQ=255) ccggccggGGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCCGGCGGAGTCACTCATGTGTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCCGGGAAATCCCCCATAACGTGTCGGAGCCGCTTAACCCGCGATTACGTCAGTgg > 4:281388/1‑148 (MQ=255) cggccggTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGca > 4:93985/1‑149 (MQ=255) aGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTcg > 7:18555/1‑149 (MQ=255) ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCgg < 1:125622/149‑1 (MQ=255) ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCgg < 6:17151/149‑1 (MQ=255) ttGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg < 1:209051/149‑1 (MQ=25) ggCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGt > 7:201695/1‑149 (MQ=255) gCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGCGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGt < 3:20359/148‑1 (MQ=255) ccAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTg > 3:285947/1‑148 (MQ=255) cAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGaa > 4:267954/1‑149 (MQ=255) aaCAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAAc > 1:366860/1‑149 (MQ=255) aCAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAAcc < 8:18555/149‑1 (MQ=255) cgcATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg > 6:29472/1‑138 (MQ=21) cgcATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCgg < 5:29472/138‑1 (MQ=21) aTCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc > 3:257006/1‑148 (MQ=255) gcgcGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAg < 7:354793/149‑1 (MQ=255) cggAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCt > 6:36742/1‑149 (MQ=255) cggAGTCACTCATGGTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCt < 7:129375/149‑1 (MQ=255) gTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCa < 8:269729/149‑1 (MQ=255) aCTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAgcg > 5:203465/1‑149 (MQ=255) tCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTgg > 6:5903/1‑95 (MQ=255) tCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTgg < 5:5903/95‑1 (MQ=255) tttCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGAt < 6:58223/149‑1 (MQ=255) cATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTAc > 8:279219/1‑149 (MQ=255) aTCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTAc < 6:338040/148‑1 (MQ=255) cTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGa > 2:279213/1‑149 (MQ=255) tttACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGa > 5:69442/1‑148 (MQ=255) cTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATg > 8:192141/1‑149 (MQ=255) tGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGc < 6:203465/148‑1 (MQ=255) tCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAg > 4:352598/1‑149 (MQ=255) cAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAAt < 3:310556/141‑1 (MQ=255) cAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAAt > 4:310556/1‑141 (MQ=255) aaCAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCtt > 3:84012/1‑149 (MQ=255) gccgcTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAgg > 7:230940/1‑149 (MQ=255) gccgcTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAgg > 8:83998/1‑149 (MQ=255) tCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGt > 6:141284/1‑149 (MQ=255) gAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAAc < 4:84012/149‑1 (MQ=255) gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa < 8:230940/149‑1 (MQ=255) gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa < 7:279219/149‑1 (MQ=255) gCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCa < 3:93985/149‑1 (MQ=255) ccccTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAg > 6:76270/1‑148 (MQ=255) cccTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCa > 6:251669/1‑149 (MQ=255) tAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc > 3:256707/1‑73 (MQ=255) tAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTcc < 4:256707/73‑1 (MQ=255) aaCTTGTAGGTGGGGTTTTACCCGCGAATACGTCAGTGGCACCACCCCCGCATTCGCGGGTGAACCCGGTCCTACAGGCTTCCTGCCAGCTCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCTCGtt < 8:196495/148‑1 (MQ=38) aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTg < 8:201695/148‑1 (MQ=255) aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGa < 1:15943/149‑1 (MQ=255) aCGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGa < 3:267954/149‑1 (MQ=255) ggAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACcgg > 6:224697/1‑149 (MQ=255) gAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTAGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACcggc > 6:60126/1‑149 (MQ=255) ggTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCGGGTAAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGcc > 5:208515/1‑149 (MQ=21) gTTCACCCGCGAATGCGGTGGTGGTGCCACTGACGTATTCGCGGGTAAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCg > 2:366383/1‑149 (MQ=21) tttACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGa > 2:60970/1‑149 (MQ=255) tACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGAt < 7:192141/148‑1 (MQ=255) gAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGTCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGt < 1:279213/149‑1 (MQ=255) aTACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTg > 6:234927/1‑148 (MQ=255) tACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGa < 5:36742/149‑1 (MQ=255) gTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGAggg > 8:262184/1‑149 (MQ=255) | CGGCCCCGGCCGGTGCGTGGGAGGTTGGCCAACAGCGCATCGGCGCGGGCGGAGTCACTCATGTTTCATCCTTTACTTTGTCCAACAGTCCGCCGCTTCAGGGAAAGCCCCCTTAACGTGTAGGAGCGGGTTTACCCGCGAATACGTCAGTGGCACCACCACCGCATTCGCGGGTGAACCCGCTCCTACAGGCTTCCTGCCAGCGCTTCGATTACCTGACAATGGCGAGCTTTTCCAAGGCCTGGTTCAACGCCAGCACGTTGACCACCGGCGGGCCGATCAATGGGTGGAGGG > NC_002947/4700626‑4700919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |