Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,734,028 T→G 7.0% E536A (GAG→GCG) ‡ sucA ← 2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,734,0280TG 7.0% 218.1 / 6.2 71E536A (GAG→GCG) ‡sucA2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base T (31/35);  new base G (2/3);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCATACAGCTCACGGGTA  >  NC_002947/4733883‑4734171
                                                                                                                                                 |                                                                                                                                               
cTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTcgc                                                                                                                                              >  4:303771/1‑149 (MQ=255)
         cTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCt                                                                                                                                     >  4:150230/1‑149 (MQ=255)
           gACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTcc                                                                                                                                   >  3:334422/1‑149 (MQ=255)
            aCGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACCGCGAGCTGTTCgtcga                                                                                                                                    <  4:186408/147‑5 (MQ=255)
            aCGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTcc                                                                                                                                   <  5:345248/148‑1 (MQ=255)
            aCGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTcc                                                                                                                                   <  3:232652/148‑1 (MQ=255)
            aCGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCt                                                                                                                                  <  8:78086/149‑1 (MQ=255)
             cGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCtt                                                                                                                                 <  5:329541/149‑1 (MQ=255)
                tGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGAc                                                                                                                              >  5:233359/1‑149 (MQ=255)
                     cACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACCAACAGCTCGCGCTTCGCCTCCTTGACCAGGc                                                                                                                         >  4:113611/1‑149 (MQ=255)
                         agctcTTCCGGCAGCTGGAGCTGCTTGGCCGACAGATCGTGCTGTGTCTTGAGGTCGAAACGGGTGTCGTGTCGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCtttt                                                                                                                     <  6:234101/145‑1 (MQ=255)
                         aaaCCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCtttt                                                                                                                     <  3:194365/149‑1 (MQ=255)
                          atccTTCCGGCCGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCttttt                                                                                                                    <  2:294463/147‑1 (MQ=255)
                          aagcTTCCGGCAGCTCGAGCTGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGTGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCttttt                                                                                                                    <  7:127924/146‑1 (MQ=255)
                          aaCCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACCGCGAGCTGTTCgtc                                                                                                                                      >  7:201170/1‑129 (MQ=255)
                          aaCCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACCGCGAGCTGTTCgtc                                                                                                                                      <  8:201170/131‑3 (MQ=255)
                            ccTTCCGGCAGCTCGAGCAGCTTGGCCGACAGTTCGTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGATGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTAc                                                                                                                  <  8:192188/149‑1 (MQ=255)
                             cTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTAcc                                                                                                                 <  3:34132/149‑1 (MQ=255)
                             cTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTAcc                                                                                                                 <  3:38813/149‑1 (MQ=255)
                             cTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTAc                                                                                                                  >  2:83290/1‑148 (MQ=255)
                              ttCCGGCAGCTCGAGCAGCTTGGCCGACACATCCTGCAGGGTCTTGAGGTCGAAACGGGTGACGTGACGCGAGGTCCAGGCATGGCCCAGGTACGGACGCAAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCtttt                                                                                                                     >  5:234101/1‑144 (MQ=255)
                                ccGGCAGCTCGCCCAGCTTGGCCGAACGATCCTTCAGGGTCTTGAGGTCGCAACGGGTGGCGTGACGCGCGGTCCAGGCATGGCCCATGGACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTTACCAGGCTTTTTACCa                                                                                                                <  4:62101/147‑1 (MQ=255)
                                ccGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCa                                                                                                                <  4:334422/147‑1 (MQ=255)
                                ccGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCa                                                                                                                <  6:319961/147‑1 (MQ=255)
                                   gCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCCGGTACGGACGCCAGTCGCCGAACAGGTCGCGGTGCGGCTCCTGGCCGAGGCGTTTTGCCACGTGc                                                                                                           >  4:8164/1‑149 (MQ=255)
                                   gCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCTTTGACCAGGCTTTTTACCACGTg                                                                                                            >  4:57773/1‑148 (MQ=255)
                                                     ccGACAGATCCTGCAGGGTCTTGAGGTCGATACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTa                                                                                                                   >  1:35759/1‑123 (MQ=255)
                                                     ccGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTa                                                                                                                   <  2:35759/123‑1 (MQ=255)
                                                       gACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCttttt                                                                                                                    <  5:58866/120‑1 (MQ=255)
                                                       gACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCttttt                                                                                                                    >  6:58866/1‑120 (MQ=255)
                                                       gACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGtt                                                                                       >  1:198238/1‑149 (MQ=255)
                                                          aGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCg                                                                                    <  2:198743/149‑1 (MQ=255)
                                                            aTCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCgg                                                                                   >  8:130272/1‑148 (MQ=255)
                                                               cTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACt                                                                               <  1:222335/149‑1 (MQ=255)
                                                                  cAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACtcgt                                                                            <  6:233359/149‑1 (MQ=255)
                                                                  cAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACtcgt                                                                            >  5:328339/1‑149 (MQ=255)
                                                                     ggTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGa                                                                         >  6:79060/1‑149 (MQ=255)
                                                                       tCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATc                                                                       <  3:303771/149‑1 (MQ=255)
                                                                         ttGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCtt                                                                     <  3:150230/149‑1 (MQ=255)
                                                                            aGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTgg                                                                   <  2:198238/148‑1 (MQ=255)
                                                                                 gAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTcc                                                                                                                                   <  3:41115/79‑1 (MQ=255)
                                                                                 gAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTcc                                                                                                                                   >  4:41115/1‑79 (MQ=255)
                                                                                 gAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAg                                                             >  1:27279/1‑149 (MQ=255)
                                                                                 gAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAg                                                             >  1:140849/1‑149 (MQ=255)
                                                                                     cGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAgcgcg                                                         <  2:140849/149‑1 (MQ=255)
                                                                                           tCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGc                                                   >  7:325423/1‑149 (MQ=255)
                                                                                           gcGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGc                                                   <  5:33437/148‑1 (MQ=255)
                                                                                             gTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGt                                                 <  2:27279/149‑1 (MQ=255)
                                                                                             gTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGt                                                 >  2:295426/1‑149 (MQ=255)
                                                                                              tGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTc                                                >  5:366494/1‑149 (MQ=255)
                                                                                                 cgcgcgGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGAt                                             <  7:130272/149‑1 (MQ=255)
                                                                                                  gcgcgGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATg                                            >  2:140856/1‑149 (MQ=255)
                                                                                                   cgcgGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGc                                           <  6:328339/149‑1 (MQ=255)
                                                                                                    gcgGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGTCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTg                                                                                      <  1:340475/105‑1 (MQ=255)
                                                                                                    gcgGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGACTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTg                                                                                      >  2:340475/1‑105 (MQ=255)
                                                                                                     cgGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCTTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGa                                         <  3:57773/149‑1 (MQ=255)
                                                                                                          cAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGc                                    >  5:38552/1‑149 (MQ=255)
                                                                                                           aGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGcc                                   >  4:214676/1‑149 (MQ=255)
                                                                                                                 ggCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGAt                              >  7:30522/1‑148 (MQ=255)
                                                                                                                  gCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTg                                                                                                  >  6:44438/1‑79 (MQ=255)
                                                                                                                  gCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTg                                                                                                  <  5:44438/79‑1 (MQ=255)
                                                                                                                  gCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCa                            <  3:113611/149‑1 (MQ=255)
                                                                                                                    ccAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGAGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAgc                          >  3:285961/1‑149 (MQ=255)
                                                                                                                           cGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCg                    <  5:79060/148‑1 (MQ=255)
                                                                                                                             gACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCa                 <  1:295426/149‑1 (MQ=255)
                                                                                                                                    gTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCATACAGCt          >  1:41045/1‑149 (MQ=255)
                                                                                                                                     tCGACCAACAGCTCGAGGTTCGGCTCCTTGACCAGGCTTTTTACAACCTGCAGGCCATTCTCCAGAGCGTTGCCGTACTCGTCGATCTTGGCCTGAGAGCGCTCGGCGTCGATGCGACCGGACTGGATCAGATGCTCGGCATACAGCt          >  8:82218/1‑148 (MQ=255)
                                                                                                                                     gagccgaACCGCGAGCTGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCATACAGCt          >  4:28077/5‑148 (MQ=255)
                                                                                                                                      agccgaACCGCGAGCTGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCATACAGCTCa        <  3:28077/146‑1 (MQ=255)
                                                                                                                                          gaACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCATACAGCTCACggg    >  4:317811/1‑149 (MQ=255)
                                                                                                                                            aCAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCATACAGCTCACGGGTa  <  3:8164/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTTCGACACCTGACGCTGGACCACGAAACCTTCCGGCAGCTCGAGCAGCTTGGCCGACAGATCCTGCAGGGTCTTGAGGTCGAAACGGGTGTCGTGACGCGCGGTCCAGGCATGGCCCAGGTACGGACGCCAGTCGACGAACAGCTCGCGGTTCGGCTCCTTGACCAGGCTTTTTACCACGTGCAGGCCATTGTCCAGCGCGTTGCGGTACTCGTCGATCTTGGCCTGAGCGCGCTCGGCGTCGATGCGACCGGCCTGGATCAGCTGCTCGGCATACAGCTCACGGGTA  >  NC_002947/4733883‑4734171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑