Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,740,841 T→A 23.7% intergenic (+78/+675) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8410TA23.7% 56.1 / 16.0 38intergenic (+78/+675)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (14/14);  new base A (3/6);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

ATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGT  >  NC_002947/4740692‑4740982
                                                                                                                                                      |                                                                                                                                             
aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑t                                                                                                                                               <  1:191760/149‑1 (MQ=255)
 tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑tt                                                                                                                                              <  4:314826/149‑1 (MQ=255)
  cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑tt                                                                                                                                              <  5:238976/148‑1 (MQ=255)
   ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGc                                                                                                                                            <  7:48944/149‑1 (MQ=255)
     ccGTCCGCGCCAGCTGTACACCGGCTAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGccc                                                                                                                                          <  5:268913/149‑1 (MQ=255)
          cgcgCCAGCTGTACAGCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAACTCAGCTCCAGAAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGaa                                                                                                                                     <  2:330529/149‑1 (MQ=255)
          cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGaa                                                                                                                                     <  1:271945/149‑1 (MQ=255)
          cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGaa                                                                                                                                     <  7:347066/149‑1 (MQ=255)
           gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAag                                                                                                                                    <  1:371339/149‑1 (MQ=255)
            cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAaga                                                                                                                                   <  6:116072/149‑1 (MQ=38)
            cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAaga                                                                                                                                   <  4:46668/149‑1 (MQ=255)
            agcCATCTGTACACCGGCGAGCAGAAGCTCGACATCGTGGCCCTGAAAGACCGAAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCTCGGGC‑AAGGCCGCGAaga                                                                                                                                   <  4:335/148‑1 (MQ=255)
                 gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCg                                                                                                                              <  2:68832/149‑1 (MQ=255)
                     tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGc                                                                                                                            <  4:310219/147‑1 (MQ=255)
                          cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAaga                                                                                                                                   >  3:230922/1‑135 (MQ=38)
                          cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAaga                                                                                                                                   <  4:230922/135‑1 (MQ=38)
                                   gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAgg                                                                                                                                 >  2:40043/1‑128 (MQ=255)
                                   gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAgg                                                                                                                                 <  1:40043/128‑1 (MQ=255)
                                             aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtttt                                                                                                   >  8:2878/1‑148 (MQ=255)
                                                     ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                                                          >  2:257417/1‑149 (MQ=255)
                                                             aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACGGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                                                   >  2:232089/1‑148 (MQ=255)
                                                               cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAg                                                                                >  6:340861/1‑149 (MQ=255)
                                                                          tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑ATGCCCGCGAAGCGGCCGGCACCGACACCACAAAACCACAGGTTTTCTCCGCCCGCTCACCCAGCCCGTTGAggg                                                                     >  5:218662/1‑149 (MQ=255)
                                                                              gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCC‑TTGGCCCGGAAGAGGGCGGCACAGACACCACAAAACCGCACTTTTTATACGCCCCCGCACGGCGCCCGTTAAgggagat                                                                 >  6:17862/1‑145 (MQ=255)
                                                                                 gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGGC‑GTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTATCCGCCCGCGAACGCAGCCCGTTAAGGGTATTGa                                                               >  4:212738/1‑148 (MQ=255)
                                                                                   cGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACg                                                            <  7:2878/149‑1 (MQ=255)
                                                                                     aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACggt                                                          <  1:257417/149‑2 (MQ=255)
                                                                                        gCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCGTGTGCCGGCCTCTTCGCGGCG‑TTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                      <  3:128804/134‑1 (MQ=255)
                                                                                                                ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCCCGAAGAGGCCGGCACACACACCACAAAACCTCAGTTTTTATCCGCCCGCGCACGCAGCCCTTTCAGGGGAATGTCAGGCGCATCAACCACGCACGTGTTGGcccc                                >  4:216418/3‑147 (MQ=255)
                                                                                                                  ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGGATTGAACGGCGCATCCACCACGAACTTGTTGGcccc                                >  1:247971/1‑146 (MQ=255)
                                                                                                                              cctgTGCCGCCCTCTTCGCGGCC‑TGGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGc                  <  3:212738/146‑1 (MQ=255)
                                                                                                                                   gCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCCCAAAACCTCAGGTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTacca             >  4:329960/1‑149 (MQ=255)
                                                                                                                                      ggCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCACCGGCACGCTACCACCt          >  3:17370/1‑149 (MQ=255)
                                                                                                                                         ctctTCGCGGGC‑AAGGCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       <  5:341333/85‑1 (MQ=37)
                                                                                                                                         ctctTCGCGGGC‑AAGGCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                       >  6:341333/1‑85 (MQ=37)
                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  7:93123/1‑149 (MQ=255)
                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  7:99267/1‑149 (MQ=255)
                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTCTTGGCCACCATCGCCGGCACGCTACCACCTGGCt      >  6:348873/1‑149 (MQ=255)
                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACATCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCGCCCACGAACTTGTTGGCCACCATCGCCGGCACGCTCCCCCCTGGCt      >  2:374619/1‑149 (MQ=255)
                                                                                                                                              cgcgGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCgg   <  2:247971/148‑1 (MQ=255)
                                                                                                                                               gcgGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgt  <  8:99267/148‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                             
ATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGT  >  NC_002947/4740692‑4740982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑