Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,740,842	T→A	21.2%	intergenic (+79/+674)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,842	0	T	A	21.2%	60.2 / 15.1	38	intergenic (+79/+674)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (16/13);  new base A (2/6);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

TCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACC  >  NC_002947/4740693‑4740989                                                                                                                                                       |                                                                                                                                                    tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑tt                                                                                                                                                     <  4:314826/149‑1 (MQ=255)  cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑tt                                                                                                                                                     <  5:238976/148‑1 (MQ=255)   ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGc                                                                                                                                                   <  7:48944/149‑1 (MQ=255)     ccGTCCGCGCCAGCTGTACACCGGCTAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGccc                                                                                                                                                 <  5:268913/149‑1 (MQ=255)          cgcgCCAGCTGTACAGCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAACTCAGCTCCAGAAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGaa                                                                                                                                            <  2:330529/149‑1 (MQ=255)          cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGaa                                                                                                                                            <  1:271945/149‑1 (MQ=255)          cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGaa                                                                                                                                            <  7:347066/149‑1 (MQ=255)           gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAag                                                                                                                                           <  1:371339/149‑1 (MQ=255)            cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAaga                                                                                                                                          <  6:116072/149‑1 (MQ=38)            cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAaga                                                                                                                                          <  4:46668/149‑1 (MQ=255)            agcCATCTGTACACCGGCGAGCAGAAGCTCGACATCGTGGCCCTGAAAGACCGAAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCTCGGGC‑AAGGCCGCGAaga                                                                                                                                          <  4:335/148‑1 (MQ=255)                 gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCg                                                                                                                                     <  2:68832/149‑1 (MQ=255)                     tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGc                                                                                                                                   <  4:310219/147‑1 (MQ=255)                          cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAaga                                                                                                                                          >  3:230922/1‑135 (MQ=38)                          cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGC‑AAGGCCGCGAaga                                                                                                                                          <  4:230922/135‑1 (MQ=38)                                   gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAgg                                                                                                                                        >  2:40043/1‑128 (MQ=255)                                   gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAgg                                                                                                                                        <  1:40043/128‑1 (MQ=255)                                             aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtttt                                                                                                          >  8:2878/1‑148 (MQ=255)                                                     ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                                                                 >  2:257417/1‑149 (MQ=255)                                                             aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACGGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                                                          >  2:232089/1‑148 (MQ=255)                                                               cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAg                                                                                       >  6:340861/1‑149 (MQ=255)                                                                          tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑ATGCCCGCGAAGCGGCCGGCACCGACACCACAAAACCACAGGTTTTCTCCGCCCGCTCACCCAGCCCGTTGAggg                                                                            >  5:218662/1‑149 (MQ=255)                                                                              gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCC‑TTGGCCCGGAAGAGGGCGGCACAGACACCACAAAACCGCACTTTTTATACGCCCCCGCACGGCGCCCGTTAAgggagat                                                                        >  6:17862/1‑145 (MQ=255)                                                                                 gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGGC‑GTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTATCCGCCCGCGAACGCAGCCCGTTAAGGGTATTGa                                                                      >  4:212738/1‑148 (MQ=255)                                                                                   cGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACg                                                                   <  7:2878/149‑1 (MQ=255)                                                                                     aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACggt                                                                 <  1:257417/149‑2 (MQ=255)                                                                                        gCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCGTGTGCCGGCCTCTTCGCGGCG‑TTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                             <  3:128804/134‑1 (MQ=255)                                                                                                                ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCCCGAAGAGGCCGGCACACACACCACAAAACCTCAGTTTTTATCCGCCCGCGCACGCAGCCCTTTCAGGGGAATGTCAGGCGCATCAACCACGCACGTGTTGGcccc                                       >  4:216418/3‑147 (MQ=255)                                                                                                                  ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGGATTGAACGGCGCATCCACCACGAACTTGTTGGcccc                                       >  1:247971/1‑146 (MQ=255)                                                                                                                              cctgTGCCGCCCTCTTCGCGGCC‑TGGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGc                         <  3:212738/146‑1 (MQ=255)                                                                                                                                   gCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCCCAAAACCTCAGGTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTacca                    >  4:329960/1‑149 (MQ=255)                                                                                                                                      ggCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCACCGGCACGCTACCACCt                 >  3:17370/1‑149 (MQ=255)                                                                                                                                         ctctTCGCGGGC‑AAGGCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                              <  5:341333/85‑1 (MQ=37)                                                                                                                                         ctctTCGCGGGC‑AAGGCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                                                              >  6:341333/1‑85 (MQ=37)                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt             >  7:93123/1‑149 (MQ=255)                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt             >  7:99267/1‑149 (MQ=255)                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTCTTGGCCACCATCGCCGGCACGCTACCACCTGGCt             >  6:348873/1‑149 (MQ=255)                                                                                                                                          tctTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACATCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCGCCCACGAACTTGTTGGCCACCATCGCCGGCACGCTCCCCCCTGGCt             >  2:374619/1‑149 (MQ=255)                                                                                                                                              cgcgGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCgg          <  2:247971/148‑1 (MQ=255)                                                                                                                                               gcgGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgt         <  8:99267/148‑1 (MQ=255)                                                                                                                                                      gtGCCCGCGAATCGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCAcc  <  8:352836/148‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                    TCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACC  >  NC_002947/4740693‑4740989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑