Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,791,726 C→T 9.3% G705D (GGC→GAC)  pvdI ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,791,7260CT 9.3% 118.4 / 2.7 43G705D (GGC→GAC) pvdInon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (18/21);  new base T (2/2);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCACGCTGGCATCGGCGCAGGCATCGAGCAGCGCACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACAC  >  NC_002947/4791579‑4791865
                                                                                                                                                   |                                                                                                                                            
agcaCGCTGGCATCGGCGCAGGCATCGAGCAGCGCACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGCTGTACCGTCTTGcc                                                                                                                                             >  4:291616/1‑149 (MQ=255)
 gcaCGCTGGCATCGGCGCAGGCATCGAGCAGCGCACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCg                                                                                                                                            >  1:335772/1‑149 (MQ=255)
   aCGCTGGCATCGGCGCAGGCATCGAGCAGCGCACCCAGGTGCGAAGGAACCATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGTCGGc                                                                                                                                          >  6:24969/1‑149 (MQ=255)
                     gCATCGAGCAGCGCACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCACTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAg                                                                                                                       <  3:200773/149‑1 (MQ=255)
                      cATCGAGCAGCGCACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAg                                                                                                                       >  7:353957/1‑148 (MQ=255)
                          gAGCAGCGCACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATg                                                                                                                   <  5:119891/148‑1 (MQ=255)
                                 gcACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGccc                                                                                                           <  1:7906/149‑1 (MQ=255)
                                         gTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTcgg                                                                                                   >  2:293455/1‑149 (MQ=255)
                                          tGCGAAGGCACGATTTTCAGCGCATTGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTcggc                                                                                                  >  4:197745/1‑149 (MQ=255)
                                          tGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTcggc                                                                                                  <  4:325068/149‑1 (MQ=255)
                                              aaGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTcggcgg                                                                                                >  8:169343/1‑147 (MQ=255)
                                                         ttCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGgaga                                                                                    <  7:181541/148‑1 (MQ=255)
                                                            aGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCAt                                                                                >  7:64971/1‑149 (MQ=255)
                                                                  tCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGaa                                                                          <  3:348220/149‑1 (MQ=255)
                                                                    gACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTTCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAgt                                                                        <  2:217447/149‑1 (MQ=255)
                                                                     aCGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAgtg                                                                       >  6:239287/1‑149 (MQ=255)
                                                                          gTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCa                                                                  <  1:178559/149‑1 (MQ=255)
                                                                          gTTTGCCGAAAGTTTGGCGGCTAAACGGTCGGCATCGTGCACGGCCTCGGGCTGCAGCATATGTACCGTCTTGCCGGCCCAGAGGGCGCCCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTc                                                                    <  1:293455/148‑1 (MQ=255)
                                                                              gacGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATc                                                              >  2:115775/3‑149 (MQ=255)
                                                                              gCCGACAGGTAGGCGGCGGAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTc                                                                    >  2:292760/1‑143 (MQ=255)
                                                                               ccGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGTCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAgtgt                                                                      >  7:262370/1‑140 (MQ=255)
                                                                               ccGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGTCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAgtgt                                                                      <  8:262370/140‑1 (MQ=255)
                                                                                         ggcggcGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGTCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCa                                                    <  5:24969/148‑1 (MQ=255)
                                                                                         ggcggcGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCa                                                    <  1:115775/148‑1 (MQ=255)
                                                                                               gAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCt                                             >  1:192994/1‑149 (MQ=255)
                                                                                                aaaCCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTc                                            <  4:270532/149‑1 (MQ=255)
                                                                                                 aaCCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCg                                           <  1:172418/149‑1 (MQ=255)
                                                                                                     gTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGAc                                        >  1:176397/1‑148 (MQ=255)
                                                                                                        ggCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGc                                    <  7:169343/149‑1 (MQ=255)
                                                                                                          cATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACa                                       <  8:212752/144‑1 (MQ=255)
                                                                                                          cATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACa                                       >  7:212752/1‑144 (MQ=255)
                                                                                                          cATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCt                                  >  7:133667/1‑149 (MQ=255)
                                                                                                          cATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCt                                  >  7:187788/1‑149 (MQ=255)
                                                                                                           aTCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCtt                                 <  4:124177/149‑1 (MQ=255)
                                                                                                               aGCACCGCCGAGCGCTGCAGCAGATGTACCGTCTTGCCGGACCAGAGCGCG‑CCAAACAGCATGGTATTACCAAGGTCGTCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAg                             <  4:230678/149‑1 (MQ=255)
                                                                                                                gCACCGCCTCGCGGTGCAGCATATGTACCGTCTTGCCGGCCCTGAGCGCG‑CCAAACTGCATGGTATGCCCATGGTCGGCGGCTGCCGTGTAGATCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAgg                            <  1:292760/149‑1 (MQ=255)
                                                                                                                 cACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAgg                            >  3:140991/1‑148 (MQ=255)
                                                                                                                      ccTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTg                      >  1:135501/1‑149 (MQ=255)
                                                                                                                      ccTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTg                      >  5:346416/1‑149 (MQ=255)
                                                                                                                          gcgcTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGc                    <  8:64971/147‑1 (MQ=255)
                                                                                                                          gcgcTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCa                  <  5:239287/149‑1 (MQ=255)
                                                                                                                                   cagATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCAt         <  7:262850/149‑1 (MQ=255)
                                                                                                                                     gATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATga       <  6:346416/149‑1 (MQ=255)
                                                                                                                                          aCCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGacac  >  2:24055/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
AGCACGCTGGCATCGGCGCAGGCATCGAGCAGCGCACCCAGGTGCGAAGGCACGATTTTCAGCGCATCGACGTGGTTTGCCGACAGGTAGGCGGCGAAACCGTCGGCATCGAGCACCGCCTCGCGCTGCAGCAGATGTACCGTCTTGCCGGCCCAGAGCGCG‑CCAAACAGCATGGTATGCCCAAGGTCGGCGGCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACAC  >  NC_002947/4791579‑4791865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑