Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,792,979 G→C 6.7% A287A (GCC→GCG)  pvdI ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,792,9790GC 6.7% 191.6 / 3.7 60A287A (GCC→GCG) pvdInon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (25/31);  new base C (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

CCGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTACTCCAGCTGGC  >  NC_002947/4792833‑4793127
                                                                                                                                                  |                                                                                                                                                    
ccGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGc                                                                                                                                                    <  3:140685/149‑1 (MQ=255)
 cGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGcc                                                                                                                                                   <  7:33683/149‑1 (MQ=255)
       gCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGACCAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAgg                                                                                                                                             >  1:63286/1‑149 (MQ=255)
        ccccTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGgc                                                                                                                                            >  8:24520/1‑149 (MQ=255)
            tCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTg                                                                                                                                        >  4:155920/1‑149 (MQ=255)
              cACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTgg                                                                                                                                       <  1:181133/148‑1 (MQ=255)
              cACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTgg                                                                                                                                       <  7:19577/148‑1 (MQ=255)
                    aCTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACg                                                                                                                                <  1:106248/149‑1 (MQ=255)
                    aCTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACg                                                                                                                                >  2:106248/1‑149 (MQ=255)
                    aCTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGCGCCTGCAGGCCCTggccaggc                                                                                                                                 <  7:9403/148‑7 (MQ=255)
                        cggttgcggttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGc                                                                                                                            <  8:299868/149‑1 (MQ=255)
                        cggttgcggttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTATTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTg                                                                                                                             <  6:131812/148‑1 (MQ=255)
                               ggttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGc                                                                                                                                                <  6:26958/122‑1 (MQ=255)
                               ggttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGc                                                                                                                                                >  5:26958/1‑122 (MQ=255)
                                gttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGAttt                                                                                                                    <  1:348861/149‑1 (MQ=255)
                                 ttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTg                                                                                                                   >  4:42255/1‑149 (MQ=255)
                                        tCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAg                                                                                                                >  6:219093/1‑145 (MQ=255)
                                        tCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAg                                                                                                                <  5:219093/145‑1 (MQ=255)
                                             acacCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTATAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCg                                                                                                       >  4:266904/1‑149 (MQ=255)
                                                 cGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCg                                                                                                   >  6:179064/1‑149 (MQ=255)
                                                  gACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGc                                                                                                  <  5:25004/149‑1 (MQ=255)
                                                   aCGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGc                                                                                                  >  2:13976/1‑148 (MQ=255)
                                                       gTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACgg                                                                                             >  2:331579/1‑149 (MQ=255)
                                                       gTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTCGCAGCTCCAGGCg                                                                                                       >  7:17209/1‑139 (MQ=255)
                                                       gTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTCGCAGCTCCAGGCg                                                                                                       <  8:17209/139‑1 (MQ=255)
                                                        tACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCACCGCTGCATCGATTTGCAGCTCCAGGCGTTCGcgccgga                                                                                            >  4:321546/1‑143 (MQ=255)
                                                           ccGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGAt                                                                                                                      >  4:182560/1‑120 (MQ=255)
                                                           ccGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGAt                                                                                                                      <  3:182560/120‑1 (MQ=255)
                                                           ccGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGc                                                                                         >  5:5177/1‑149 (MQ=255)
                                                                aCTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGc                                                                                    <  3:155920/149‑1 (MQ=255)
                                                                 cTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACTGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCa                                                                                   <  7:151898/149‑1 (MQ=255)
                                                                    gCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAgcg                                                                                >  5:351907/1‑149 (MQ=255)
                                                                           tAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGa                                                                           <  2:63286/147‑1 (MQ=255)
                                                                              cGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAg                                                                      <  1:167789/149‑1 (MQ=255)
                                                                                 tACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCt                                                                                        <  6:9871/128‑1 (MQ=255)
                                                                                  acaacaAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCa                                                                                                <  8:212822/119‑1 (MQ=255)
                                                                                  acaacaAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCa                                                                                                >  7:212822/1‑119 (MQ=255)
                                                                                    aacaaGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATc                                                                >  6:193528/1‑149 (MQ=255)
                                                                                      caaGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGc                                                              >  5:7399/1‑149 (MQ=255)
                                                                                      caaGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGc                                                              >  6:307544/1‑149 (MQ=255)
                                                                                        aGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTc                                                            >  2:141797/1‑149 (MQ=255)
                                                                                         ggTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAg                                                                      >  6:208164/1‑138 (MQ=255)
                                                                                         ggTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAg                                                                      <  5:208164/138‑1 (MQ=255)
                                                                                           tCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCgg                                                          >  4:207969/1‑148 (MQ=255)
                                                                                                aaaGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAAtt                                                     <  7:24520/148‑1 (MQ=255)
                                                                                                            agcaCCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGggtg                                        <  1:7956/149‑1 (MQ=255)
                                                                                                            agcaCCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGggtg                                        <  5:193528/149‑1 (MQ=255)
                                                                                                                ccacGAACAGCGTCACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCg                                    >  3:261322/1‑149 (MQ=255)
                                                                                                                 cacGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACg                                                                         <  4:160133/111‑1 (MQ=255)
                                                                                                                           gTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAgat                          >  8:262215/1‑148 (MQ=255)
                                                                                                                                   gtgCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAAc                  <  6:351907/148‑1 (MQ=255)
                                                                                                                                    tgCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCa                <  6:7399/149‑1 (MQ=255)
                                                                                                                                      ccgccTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAg               <  7:92/148‑1 (MQ=255)
                                                                                                                                       cgccTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTa             <  8:7430/149‑1 (MQ=255)
                                                                                                                                        gttggcccAGCGCCTGCAGGCCCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAgatga                        <  3:97735/131‑1 (MQ=255)
                                                                                                                                        gttggcccAGCGCCTGCAGGCCCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAgatga                        >  4:97735/7‑137 (MQ=255)
                                                                                                                                        gttggcccAGCGCCTGCAGGCCCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTAc            >  2:265564/7‑149 (MQ=255)
                                                                                                                                        gccTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTAc            <  7:262215/149‑1 (MQ=255)
                                                                                                                                        gccTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTAc            <  5:179064/149‑1 (MQ=255)
                                                                                                                                                aGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTACTCCAGCTg    <  3:42255/149‑1 (MQ=255)
                                                                                                                                                  ggCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTACTCCAGCTGgc  >  3:69572/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTACTCCAGCTGGC  >  NC_002947/4792833‑4793127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑