Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,872,311 A→G 7.1% Y229C (TAC→TGC)  guaD → guanine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,872,3110AG 7.1% 159.9 / 2.4 56Y229C (TAC→TGC) guaDguanine deaminase
Reads supporting (aligned to +/- strand):  ref base A (28/24);  new base G (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TCGGGCTATGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTACACCTGTG  >  NC_002947/4872163‑4872455
                                                                                                                                                    |                                                                                                                                                
tCGGGCTATGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTa                                                                                                                                                  >  4:111962/1‑149 (MQ=255)
  gggCTATGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACa                                                                                                                                                >  1:199938/1‑149 (MQ=255)
  gggCTATGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACa                                                                                                                                                <  5:351782/149‑1 (MQ=255)
  gggCTATGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACa                                                                                                                                                <  5:357193/149‑1 (MQ=255)
        tGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGca                                                                                                                                            <  7:9544/147‑1 (MQ=255)
           cGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACg                                                                                                                                        >  5:173006/1‑148 (MQ=255)
                       aCTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCTGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGaa                                                                                                                            >  4:235849/1‑148 (MQ=255)
                           aTCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCt                                                                                                                       <  3:295466/149‑1 (MQ=255)
                              gAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCCGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCaa                                                                                                                    <  3:235849/149‑1 (MQ=255)
                               aGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGGACACCGGAACTATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAATACCTCAAg                                                                                                                   <  4:153475/149‑1 (MQ=255)
                               aGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                   <  2:199938/149‑1 (MQ=255)
                               aGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                   <  4:147701/149‑1 (MQ=255)
                                 cgcTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAgg                                                                                                                  >  6:177016/1‑148 (MQ=255)
                                     ggcatggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                   >  7:118061/1‑143 (MQ=255)
                                     ggcatggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                   <  8:118061/143‑1 (MQ=255)
                                      gcatggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATc                                                                                                             <  5:177016/148‑1 (MQ=255)
                                        atggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGaaa                                                                                                               <  4:255931/144‑1 (MQ=255)
                                        atggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGaaa                                                                                                               >  3:255931/1‑144 (MQ=255)
                                         tggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACt                                                                                                         <  6:94692/149‑1 (MQ=255)
                                             aaGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGt                                                                                                     >  3:352932/1‑149 (MQ=255)
                                                ggCCGCCGGCACTATGCAGTCACCCCGCGCTTCGCCCCCACCAGCACACCGGAACAATTGACCCTGGCCGGCCACTTGCTCAAGGAACACCCCGGCGAGTACATCCACACGCCCCTGTCGGAAACCCTCAACGAAATCGCCTGGGTc                                                                                                    >  3:237810/1‑147 (MQ=255)
                                                  ccgccTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCCCCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGt                                                                                                >  4:114857/1‑149 (MQ=255)
                                                    gccTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACAt                                                                                                                                               <  6:269964/100‑1 (MQ=255)
                                                    gccTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACAt                                                                                                                                               >  5:269964/1‑100 (MQ=255)
                                                                 aGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAg                                                                                  >  3:34646/1‑148 (MQ=255)
                                                                 aGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAg                                                                                  >  5:251710/1‑148 (MQ=255)
                                                                     aCCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAg                                                                               >  6:81500/1‑147 (MQ=255)
                                                                       cccGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCCGAAAACCTCAAGGAAATCGACTGGGTCAAGGCGCTGTTCCCCGCGCAGAAgg                                                                           >  7:319051/1‑149 (MQ=255)
                                                                       cccGCGCTTCGCCCCAAACAGCAAAAATGAACAATTGACCCTGGCAGGCCAATTGCTCAAGGAACACACCGGCGTTTAAATCAACACGCACCTGTCGCAAAACCTAAAGGAAATCGAATGGGAAAAGTCGATGTTCCCCGAGCAGAAgg                                                                           >  4:56078/1‑149 (MQ=255)
                                                                           cgcTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCt                                                                                            <  2:147762/128‑1 (MQ=255)
                                                                                  ccccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGc                                                                                             <  7:189603/120‑1 (MQ=255)
                                                                                  ccccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGc                                                                                             >  8:189603/1‑120 (MQ=255)
                                                                                  ccccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGAc                                                                <  4:352932/149‑1 (MQ=255)
                                                                                    ccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGt                                                              >  8:279396/1‑149 (MQ=255)
                                                                                        ccAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTAc                                                          <  4:34646/149‑1 (MQ=255)
                                                                                             acacCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCa                                                     >  5:65682/1‑149 (MQ=255)
                                                                                               acCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACt                                                   >  7:194342/1‑149 (MQ=255)
                                                                                                 cGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTc                                                 >  4:99300/1‑149 (MQ=255)
                                                                                                 cGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTc                                                 <  6:173006/149‑1 (MQ=255)
                                                                                                           gACCCTGGCCGCCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGATATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGctgctg                                        <  8:70758/148‑1 (MQ=255)
                                                                                                             cccTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGc                                     >  7:139981/1‑149 (MQ=255)
                                                                                                              ccTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGc                                     >  7:137645/1‑148 (MQ=255)
                                                                                                                  gccggccAATTGCTCAAGGAACACCCCGGCGTGTACATGCACAAGCACCTGTAGGAAAACCTCAAGGAAATCGACTGTGTCAAGTCGCTGTTCCCCGAGCATAAGTGTTATCTGGACTTCTACGATCACTTCGAGCTGCTGGGCGAgc                                 <  3:56078/148‑1 (MQ=255)
                                                                                                                    cggccAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCt                              >  1:343768/1‑149 (MQ=255)
                                                                                                                      gccAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCg                            >  4:22646/1‑149 (MQ=255)
                                                                                                                             gCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGGTGGGCGAGCGCTCGGTGTTTg                     >  1:180411/1‑149 (MQ=255)
                                                                                                                             gCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGttt                      <  8:139981/148‑1 (MQ=255)
                                                                                                                              cTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGc                    >  6:230164/1‑149 (MQ=255)
                                                                                                                               tCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGcc                   >  6:265949/1‑149 (MQ=255)
                                                                                                                                       gacaggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCg            >  7:260201/8‑148 (MQ=255)
                                                                                                                                       gacaggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGt           <  8:260201/142‑1 (MQ=255)
                                                                                                                                       cacCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGTGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGt           <  5:230164/149‑1 (MQ=255)
                                                                                                                                          cccGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTaca        >  7:77247/1‑149 (MQ=255)
                                                                                                                                           ggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTacac       >  5:31496/4‑149 (MQ=255)
                                                                                                                                           ggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTacac       <  6:31496/146‑1 (MQ=255)
                                                                                                                                             ggCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTACACCt     <  5:265949/149‑1 (MQ=255)
                                                                                                                                               cGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGC‑TACACCtgtg  <  2:343768/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCGGGCTATGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTACACCTGTG  >  NC_002947/4872163‑4872455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑