Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,872,316	C→T	8.0%	H231Y (CAC→TAC)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,872,316	0	C	T	8.0%	144.1 / 2.4	51	H231Y (CAC→TAC)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base T (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

TGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTACACCTGTGCGAC  >  NC_002947/4872171‑4872459                                                                                                                                                  |                                                                                                                                                tGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGca                                                                                                                                                <  7:9544/147‑1 (MQ=255)    cGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACg                                                                                                                                            >  5:173006/1‑148 (MQ=255)                aCTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCTGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGaa                                                                                                                                >  4:235849/1‑148 (MQ=255)                    aTCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCt                                                                                                                           <  3:295466/149‑1 (MQ=255)                       gAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCCGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCaa                                                                                                                        <  3:235849/149‑1 (MQ=255)                        aGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGGACACCGGAACTATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAATACCTCAAg                                                                                                                       <  4:153475/149‑1 (MQ=255)                        aGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                       <  2:199938/149‑1 (MQ=255)                        aGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                       <  4:147701/149‑1 (MQ=255)                          cgcTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAgg                                                                                                                      >  6:177016/1‑148 (MQ=255)                              ggcatggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                       >  7:118061/1‑143 (MQ=255)                              ggcatggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAg                                                                                                                       <  8:118061/143‑1 (MQ=255)                               gcatggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATc                                                                                                                 <  5:177016/148‑1 (MQ=255)                                 atggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGaaa                                                                                                                   >  3:255931/1‑144 (MQ=255)                                 atggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGaaa                                                                                                                   <  4:255931/144‑1 (MQ=255)                                  tggcaAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACt                                                                                                             <  6:94692/149‑1 (MQ=255)                                      aaGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGt                                                                                                         >  3:352932/1‑149 (MQ=255)                                         ggCCGCCGGCACTATGCAGTCACCCCGCGCTTCGCCCCCACCAGCACACCGGAACAATTGACCCTGGCCGGCCACTTGCTCAAGGAACACCCCGGCGAGTACATCCACACGCCCCTGTCGGAAACCCTCAACGAAATCGCCTGGGTc                                                                                                        >  3:237810/1‑147 (MQ=255)                                           ccgccTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCCCCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGt                                                                                                    >  4:114857/1‑149 (MQ=255)                                                          aGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAg                                                                                      >  5:251710/1‑148 (MQ=255)                                                          aGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAg                                                                                      >  3:34646/1‑148 (MQ=255)                                                              aCCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAg                                                                                   >  6:81500/1‑147 (MQ=255)                                                                cccGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCCGAAAACCTCAAGGAAATCGACTGGGTCAAGGCGCTGTTCCCCGCGCAGAAgg                                                                               >  7:319051/1‑149 (MQ=255)                                                                cccGCGCTTCGCCCCAAACAGCAAAAATGAACAATTGACCCTGGCAGGCCAATTGCTCAAGGAACACACCGGCGTTTAAATCAACACGCACCTGTCGCAAAACCTAAAGGAAATCGAATGGGAAAAGTCGATGTTCCCCGAGCAGAAgg                                                                               >  4:56078/1‑149 (MQ=255)                                                                    cgcTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCt                                                                                                <  2:147762/128‑1 (MQ=255)                                                                           ccccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGc                                                                                                 <  7:189603/120‑1 (MQ=255)                                                                           ccccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGc                                                                                                 >  8:189603/1‑120 (MQ=255)                                                                           ccccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGAc                                                                    <  4:352932/149‑1 (MQ=255)                                                                             ccAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGt                                                                  >  8:279396/1‑149 (MQ=255)                                                                                 ccAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTAc                                                              <  4:34646/149‑1 (MQ=255)                                                                                      acacCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCa                                                         >  5:65682/1‑149 (MQ=255)                                                                                        acCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACt                                                       >  7:194342/1‑149 (MQ=255)                                                                                          cGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTc                                                     >  4:99300/1‑149 (MQ=255)                                                                                          cGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTc                                                     <  6:173006/149‑1 (MQ=255)                                                                                                    gACCCTGGCCGCCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGATATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGctgctg                                            <  8:70758/148‑1 (MQ=255)                                                                                                      cccTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGc                                         >  7:139981/1‑149 (MQ=255)                                                                                                       ccTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGc                                         >  7:137645/1‑148 (MQ=255)                                                                                                           gccggccAATTGCTCAAGGAACACCCCGGCGTGTACATGCACAAGCACCTGTAGGAAAACCTCAAGGAAATCGACTGTGTCAAGTCGCTGTTCCCCGAGCATAAGTGTTATCTGGACTTCTACGATCACTTCGAGCTGCTGGGCGAgc                                     <  3:56078/148‑1 (MQ=255)                                                                                                             cggccAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCt                                  >  1:343768/1‑149 (MQ=255)                                                                                                               gccAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCg                                >  4:22646/1‑149 (MQ=255)                                                                                                                      gCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGGTGGGCGAGCGCTCGGTGTTTg                         >  1:180411/1‑149 (MQ=255)                                                                                                                      gCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGttt                          <  8:139981/148‑1 (MQ=255)                                                                                                                       cTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGc                        >  6:230164/1‑149 (MQ=255)                                                                                                                        tCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGcc                       >  6:265949/1‑149 (MQ=255)                                                                                                                                gacaggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCg                >  7:260201/8‑148 (MQ=255)                                                                                                                                gacaggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGt               <  8:260201/142‑1 (MQ=255)                                                                                                                                cacCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGTGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGt               <  5:230164/149‑1 (MQ=255)                                                                                                                                   cccGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTaca            >  7:77247/1‑149 (MQ=255)                                                                                                                                    ggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTacac           >  5:31496/4‑149 (MQ=255)                                                                                                                                    ggtgCGTGTGCATGTACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTacac           <  6:31496/146‑1 (MQ=255)                                                                                                                                      ggCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTACACCt         <  5:265949/149‑1 (MQ=255)                                                                                                                                        cGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGC‑TACACCtgtg      <  2:343768/149‑1 (MQ=255)                                                                                                                                              cATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTACACCTGTGcgac  <  3:22646/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                TGCCGAAAGCAAGGCACTGATCGAGCGCTGGCATGGCAAGGGCCGCCTGCACTATGCAGTCACCCCGCGCTTCGCCCCAACCAGCACACCGGAACAATTGACCCTGGCCGGCCAATTGCTCAAGGAACACCCCGGCGTGTACATGCACACGCACCTGTCGGAAAACCTCAAGGAAATCGACTGGGTCAAGTCGCTGTTCCCCGAGCAGAAGGGTTATCTGGACGTCTACGATCACTTCGAGCTGCTGGGCGAGCGCTCGGTGTTTGCCCACGGCGTACACCTGTGCGAC  >  NC_002947/4872171‑4872459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑