| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,905,499 | C→T | 7.2% | intergenic (‑93/+35) | PP_4313 ← / ← PP_4314 | peptidylprolyl isomerase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,905,499 | 0 | C | T | 7.2% | 157.6 / 2.5 | 55 | intergenic (‑93/+35) | PP_4313/PP_4314 | peptidylprolyl isomerase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (21/30); new base T (2/2); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
GGCAGCTTTGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAG > NC_002947/4905353‑4905642 | ggCAGCTTTGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGcc > 3:67822/1‑148 (MQ=255)ggCAGCTTTGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGcc > 5:43589/1‑148 (MQ=255) gCAGCTTTGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGa > 8:318592/1‑149 (MQ=255) caaagctGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCAAGCAGAATTGTACGGAGGCATT‑CGCCGAc > 6:102608/7‑149 (MQ=255) cGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAATAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCt < 4:60184/149‑1 (MQ=255) tctcCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTcc < 3:9033/149‑1 (MQ=255) ctcCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCg > 1:98201/1‑149 (MQ=255) ccTCGACCATCGCGAAGATCTGCAGTTCTTGGCTCTCGCCCCATACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATTCCGTCGGC‑GAATGCCTcc < 1:316103/146‑1 (MQ=255) ccTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTa > 2:98282/1‑148 (MQ=255) ccTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTa > 4:82896/1‑148 (MQ=255) ccTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTa > 3:358196/1‑148 (MQ=255) cTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACa < 3:192223/149‑1 (MQ=255) gACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGa < 4:67822/148‑1 (MQ=255) gACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGAc < 5:354117/149‑1 (MQ=255) ccAGGTCGATGATCTGCAGTTATTGGCTCACT‑GGTAAAATCCAAACGCTTAAAAACAAAACCGACCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGAAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACAt < 1:238174/149‑1 (MQ=255) gatgatCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTg < 4:137042/149‑1 (MQ=255) attatCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAATCCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGa < 7:329935/146‑1 (MQ=255) atgatCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTg > 3:193397/1‑148 (MQ=255) atgatCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGa < 1:98282/149‑1 (MQ=255) cAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCa < 5:102608/145‑1 (MQ=255) aGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCGCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGtt < 3:95097/138‑1 (MQ=255) aGTTCTTGGCTCACC‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGtt > 4:95097/1‑138 (MQ=255) tGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGc < 3:121990/148‑1 (MQ=255) ggCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGc < 8:322566/147‑1 (MQ=255) g‑ggTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACAt < 5:1712/118‑1 (MQ=255) g‑ggTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACAt > 6:1712/1‑118 (MQ=255) gTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGATGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACctc > 4:260800/1‑149 (MQ=255) cacTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTg < 4:193397/149‑1 (MQ=255) cTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGc < 1:206016/149‑1 (MQ=255) cTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGc < 5:271642/149‑1 (MQ=255) cTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGc > 3:147982/1‑149 (MQ=255) cAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTGACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGCCGTTGATTCAGCTGTTCTGCTCGAACACCTCTTGGCCg > 8:192018/1‑148 (MQ=255) cAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGcc < 2:98201/147‑1 (MQ=255) aaaCGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGa < 7:318592/149‑1 (MQ=255) cGCTGAAAAACAAACCCGCCATTTTCTCAGGAATGCAGCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAg < 4:229919/149‑1 (MQ=255) tGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATTCCGTCGGC‑GAATGCCTcc > 5:282528/1‑91 (MQ=37) tGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATTCCGTCGGC‑GAATGCCTcc < 6:282528/91‑1 (MQ=37) aaaaaCAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTg < 8:216004/135‑1 (MQ=255) aaaaaCAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTg > 7:216004/1‑135 (MQ=255) aaaacaaaacCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTc < 6:358592/87‑1 (MQ=255) caaaacCGCCCTTTTCTCAGGAATGCACCGGTTTGCGCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTgg > 1:56906/1‑148 (MQ=255) caaaacCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGATGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTc < 3:260800/149‑1 (MQ=255) aacCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCtca > 2:238757/1‑147 (MQ=255) ttttCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCg > 4:335438/1‑147 (MQ=255) ttCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAg < 4:147982/148‑1 (MQ=255) ttCTCAGGAATGCACCGGTTTC‑GCACGCTCACGCAGAATTGTACGCAGGCATT‑CGCCGACGGAAGGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGTCAGCTCCTTGGTCTGATTGCCGCAg < 2:323264/148‑1 (MQ=255) tcAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCTTTGGTCTGATTGGCGAAGCAg > 1:162259/1‑148 (MQ=255) aGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTAc > 4:321173/1‑149 (MQ=255) tGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGGATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCAcgc > 5:360338/1‑149 (MQ=255) aCCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGc < 2:44921/131‑1 (MQ=255) aCCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGc > 1:44921/1‑131 (MQ=255) gTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGACTCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTtc < 5:174129/149‑1 (MQ=255) aaGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTcga < 3:82896/149‑1 (MQ=255) cACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGaa < 4:358196/148‑1 (MQ=255) tACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTc > 6:127035/1‑148 (MQ=255) ggAGGCATTCCGTCGGC‑GAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGaa > 7:34391/1‑148 (MQ=255) aTT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCTTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAg < 2:162259/149‑1 (MQ=255) | GGCAGCTTTGCCGTCTCCCTCGACCAGGTCGATGATCTGCAGTTCTTGGCTCACG‑GGTACACTCCAAACGCTGAAAAACAAAACCGCCCTTTTCTCAGGAATGCACCGGTTTG‑GCAAGTTCACGCAGAATTGTACGGAGGCATT‑CGCCGACGGAATGCCTCCGTACAGACATGACGTTGAATCAGCTGTTCTGCTCGAACACCTCTTGGCCGGACAGCTCCTTGGTCTGATTGGCGAAGCAGTACCACGCGGGTTTCTCTTCGACGAAGATCTGCAGGTCGAAATCCCAG > NC_002947/4905353‑4905642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |