| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 461,023 | C→A | 7.2% | R481L (CGG→CTG) | pqqF ← | coenzyme PQQ synthesis protein F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 461,023 | 0 | C | A | 7.2% | 296.1 / 11.1 | 97 | R481L (CGG→CTG) | pqqF | coenzyme PQQ synthesis protein F |
| Reads supporting (aligned to +/- strand): ref base C (44/46); new base A (3/4); total (47/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01 | |||||||||||
CCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCG > NC_002947/460877‑461165 | ccGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgc < 3:22104/149‑1 (MQ=255) cTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcag > 6:360864/1‑148 (MQ=255) ggggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcagca < 7:102759/147‑1 (MQ=255) gCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCACGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGATTGTTCCAC‑TGA‑CCGCAGCActgctgcag > 6:365454/1‑147 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCa < 6:232590/148‑1 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CAGCAGCAGTGCTGCGGGCa < 1:917/148‑1 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CAGCAGCAGTGCTGCGGGCa < 6:137725/148‑1 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAgg < 2:190644/149‑1 (MQ=255) ccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGc > 6:359095/1‑149 (MQ=255) ccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGc < 5:359095/149‑1 (MQ=255) ccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGc > 1:217701/1‑149 (MQ=255) aCCGCGTCTGGCAGTTGCTTGAGCAGGGGCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCTTCAACGCCAAGGCTTGTTCCAC‑TGC‑CAGCAGCAGTGCTGCGGGCAgtggaa < 2:168440/148‑6 (MQ=255) cgcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGgcg > 8:313022/1‑148 (MQ=255) gcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGgcg > 7:95829/1‑147 (MQ=255) gTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGt < 8:187715/149‑1 (MQ=255) tCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTg > 3:264521/1‑149 (MQ=255) gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAAc > 8:350678/1‑149 (MQ=255) gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAAc > 8:17713/1‑149 (MQ=255) cAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt < 4:235220/149‑1 (MQ=255) aGTTGCTTGAGCAGGGCCCGGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTg < 6:247838/149‑1 (MQ=255) gTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGc < 4:185403/149‑1 (MQ=255) ttGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGc > 3:208209/1‑148 (MQ=255) gCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCa < 6:232654/148‑1 (MQ=255) cTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcag > 7:299107/1‑111 (MQ=255) cTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcag < 8:299107/111‑1 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcag < 7:183382/110‑1 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcag > 8:183382/1‑110 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcag > 6:318172/1‑110 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCgcag < 5:318172/110‑1 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTa > 3:224435/1‑149 (MQ=255) tGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTAt > 3:256003/1‑149 (MQ=255) gggCGCTGATGGGGATGAGTGCCGGGGGGAGCGCCGTGCAGGGCAGCGAGCAACTGGCAGGTGGCTTGAGCATCTAGGCCTAGGCTTGTTCAAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCGCCg < 8:159340/149‑1 (MQ=255) ggCCCTGATCCCCATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCgg > 5:80197/1‑149 (MQ=255) cccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCa > 2:57638/1‑149 (MQ=255) cccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCa < 6:50335/149‑1 (MQ=255) ccTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCAc > 5:279109/1‑149 (MQ=255) tGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCActg > 8:220542/1‑149 (MQ=255) gATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAAc < 4:191431/127‑1 (MQ=255) gATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAAc > 3:191431/1‑127 (MQ=255) aTGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCActg < 4:256003/147‑1 (MQ=255) ggggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGCTCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCActgct < 1:108443/148‑1 (MQ=255) aTCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTg > 2:235933/1‑149 (MQ=255) cAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTa < 3:213113/126‑1 (MQ=255) cAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTa > 4:213113/1‑126 (MQ=255) gggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGt > 3:370772/1‑149 (MQ=255) ggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCGGGCCGGCGCAGTGCAACt > 2:63356/1‑116 (MQ=255) ggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCGGGCCGGCGCAGTGCAACt < 1:63356/116‑1 (MQ=255) ggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGtt < 4:300998/149‑1 (MQ=255) ccGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGc < 8:31106/149‑1 (MQ=255) ggggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACg < 2:179748/149‑1 (MQ=255) gCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAg < 7:220542/149‑1 (MQ=255) cAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTcaca < 5:360864/149‑1 (MQ=255) aGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACaa < 8:95829/149‑1 (MQ=255) gggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAAc < 6:80197/149‑1 (MQ=255) ggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACg > 1:329634/1‑149 (MQ=255) cagccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCActg < 3:36391/112‑1 (MQ=255) cagccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCActg > 4:36391/1‑112 (MQ=255) agccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTc < 8:261446/149‑1 (MQ=255) ccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt < 7:350678/149‑1 (MQ=255) ccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt > 3:233420/1‑149 (MQ=255) ccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt < 2:76953/149‑1 (MQ=255) ccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt < 7:17713/149‑1 (MQ=255) agcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGc < 6:279109/149‑1 (MQ=255) cAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCTGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAg < 4:208209/149‑1 (MQ=255) cAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCa < 8:57251/115‑1 (MQ=255) cAACTGGCAGGTGGCTTGAGCAACAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCa > 7:57251/1‑115 (MQ=255) tGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGgc > 2:349534/1‑149 (MQ=255) gCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGgc < 1:57638/147‑1 (MQ=255) gCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGgc < 6:87786/147‑1 (MQ=255) gTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCaa > 2:22773/1‑73 (MQ=255) gTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCaa < 1:22773/73‑1 (MQ=255) gTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAgg > 5:160919/1‑149 (MQ=255) gTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAgg < 2:217701/149‑1 (MQ=255) cTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCt > 3:195325/1‑149 (MQ=255) cTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCt > 6:164982/1‑149 (MQ=255) ttGAGCATCAAGGCCAAGGCTTGTTCCACGTGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCt > 1:28981/1‑149 (MQ=255) ttGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTg > 5:161420/1‑149 (MQ=255) gAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCAGTACAGCAGtgccg > 2:53347/1‑100 (MQ=255) gAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCAGTACAGCAGtgccg < 1:53347/104‑5 (MQ=255) gCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTc > 3:166639/1‑148 (MQ=255) cATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCa < 1:205263/149‑1 (MQ=255) cATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCa < 4:233420/149‑1 (MQ=255) cATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGACCTGTTCCa < 2:329634/149‑1 (MQ=255) aTCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAg > 1:89488/1‑149 (MQ=255) caaggccaaggcTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGca > 5:143557/1‑149 (MQ=255) caaggcTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt > 2:275622/1‑149 (MQ=255) aaggcTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTg > 6:326054/1‑149 (MQ=255) gcTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTaa > 3:308185/1‑149 (MQ=255) ttGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACg > 5:126947/1‑149 (MQ=255) gTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACgg < 2:28981/148‑1 (MQ=255) ttCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACGCGCTGTACCGGCt > 4:272732/1‑149 (MQ=255) ttCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCt < 5:326054/149‑1 (MQ=255) cggcc‑TGC‑CAGCAGCAGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTg > 4:85117/5‑149 (MQ=255) cggcc‑TGC‑CAGCAGCAGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTg > 7:192044/5‑149 (MQ=255) aC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGgcg < 4:166639/149‑1 (MQ=255) tGC‑CAGCAGCAGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACgg > 4:73682/1‑141 (MQ=255) tGC‑CAGCAGCAGTGCTGCGGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACgg < 3:73682/141‑1 (MQ=255) | CCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCAC‑TGC‑CCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCG > NC_002947/460877‑461165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |