Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,104,900 G→A 7.5% A376T (GCA→ACA)  yhhS → carbohydrate efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,104,9000GA 7.5% 151.4 / 2.3 54A376T (GCA→ACA) yhhScarbohydrate efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base A (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AACCAAGTGTCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTT  >  NC_002947/5104753‑5105047
                                                                                                                                                   |                                                                                                                                                   
aaCCAAGTGTCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCgg                                                                                                                                                     <  3:347303/148‑1 (MQ=255)
aaCCAAGTGTCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTACATAGACTTGTCACTGGGGGTGACCGGGCCAATGCACGGTGCGGTTGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGCCGGCTGCgg                                                                                                                                                     >  6:328962/1‑148 (MQ=255)
  ccAAGTGTCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAg                                                                                                                                                  >  3:107744/1‑149 (MQ=255)
  ccAAGTGTCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAg                                                                                                                                                  >  7:267778/1‑149 (MQ=255)
   cAAGTGTCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGc                                                                                                                                                 >  5:281571/1‑149 (MQ=255)
         tCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCgg                                                                                                                                           >  5:130829/1‑149 (MQ=255)
         tCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCgg                                                                                                                                           >  5:316381/1‑149 (MQ=255)
         tCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTAATCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCgg                                                                                                                                           >  4:99899/1‑149 (MQ=255)
              ggcTAACCGCGGGGGGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTgg                                                                                                                                      >  7:319513/1‑149 (MQ=255)
               gcTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGAGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGgt                                                                                                                                     >  1:120798/1‑149 (MQ=255)
                    ccGCGGGGGGGCGGTTGGGTCGTATTCCGTGTTCATGTACTGGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGa                                                                                                                                <  8:287614/149‑1 (MQ=255)
                    ccGCGGGGCGGGGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGa                                                                                                                                <  4:101940/149‑1 (MQ=255)
                    ccGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGtt                                                                                                                                  >  4:181651/1‑147 (MQ=255)
                    ccGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGa                                                                                                                                <  2:94892/149‑1 (MQ=255)
                    ccGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGa                                                                                                                                <  4:107744/149‑1 (MQ=255)
                    ccGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGa                                                                                                                                <  6:281571/149‑1 (MQ=255)
                    ccGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGa                                                                                                                                <  2:163026/149‑1 (MQ=255)
                            cggcggTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTAt                                                                                                                        <  6:316381/149‑1 (MQ=255)
                              gcggTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTAttt                                                                                                                      <  1:185419/149‑1 (MQ=255)
                                ggTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGt                                                                                                                    <  4:47018/149‑1 (MQ=255)
                                 gTGGGGTCGTATTCGGTGTTGATCGTCTTGTCTGTGGGGGTGAGCGGGCCATTGGTCGGTGCGGTGGCGGCGGTGTTCGTCTTTTCTTCGATGTTTCTGTTCGCGGAGGCTGCGTCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTa                                                                                                                   <  5:328962/149‑1 (MQ=255)
                                    ggggCGTATTCTCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCg                                                                                                                <  8:267778/149‑1 (MQ=255)
                                     gggCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGc                                                                                                               <  6:130829/149‑1 (MQ=255)
                                     gggCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGAGGCGGGGTTCCGCTTTGCTTCGATGTTACTGTTCGCGGCGGCTGCGGCAGCCTGCGGGTTGGTGTTGAGCCTCTATATGTACCGc                                                                                                               >  3:86912/1‑149 (MQ=255)
                                         gTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGACAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAg                                                                                                           >  5:14064/1‑149 (MQ=255)
                                         gTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGATTTGCTTCGATGTTTCTGTTCTCGGAGGCTGCGACAGCGTGCGGGTTGGTGTTGAGCCACTATTTGTACCGCCAAg                                                                                                           >  5:19326/1‑149 (MQ=255)
                                            ttCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGc                                                                                                        >  2:107971/1‑149 (MQ=255)
                                               gCTGTTCATCGACTTGTCACTGGGGGGGACCGGGCCATTGGTCGGGGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTa                                                                                                     <  2:82178/149‑1 (MQ=255)
                                                cTGTTCATCGTGTTGTCAGTGGGGGTGACCGGGGCTTTGGTCGGTGCGTTGGCGGCGGGGTTCGGGTTTGCTTCGATGTTTCTGTGCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTag                                                                                                    <  4:330068/149‑1 (MQ=255)
                                                     cATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGGGGGTTGGTGGTGAGCCTCTATTTGTACCGCCAAGGGCGTaggga                                                                                                 >  4:315805/1‑147 (MQ=255)
                                                       tCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTagggacagg                                                                                             >  1:191380/1‑149 (MQ=255)
                                                            ttGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCg                                                                                        >  4:320739/1‑149 (MQ=255)
                                                                     ggggTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCGTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGa                                                                               <  4:55666/149‑1 (MQ=255)
                                                                             cGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGa                                                                        >  6:236794/1‑148 (MQ=255)
                                                                              gggCCATTGGTCGGGGCGGTGGCGGCGGGGGTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGaaa                                                                      <  3:222149/149‑1 (MQ=255)
                                                                                gCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGAGGGTTCTGTTCGCGGCGGCTGCGGCAGCGGGCGGGTTGGGGTTGAGCCTCTAATTGTACCGCCAAGGGCGAAGGGTCAGGGACCGTACAACAGAAGCCTGAAAGc                                                                    >  6:267608/1‑149 (MQ=255)
                                                                                 ccATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGACAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGcc                                                                   <  6:14064/149‑1 (MQ=255)
                                                                                 ccATTGGTCGGGGCGGGGGCGGCGGGGTTCGGCTTTGCTTCGATGTTGCTGTTCGCGGCGGCTGCGACAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCTAGGGCGTAGGGACAGGGACCGTACAACAGAAGCGTGAAAGcc                                                                   <  6:19326/149‑1 (MQ=255)
                                                                                  cATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGcc                                                                   <  1:184122/148‑1 (MQ=255)
                                                                                  cATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGcc                                                                   >  2:184122/1‑148 (MQ=255)
                                                                                  cATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCg                                                                  <  8:319513/149‑1 (MQ=255)
                                                                                  cATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCg                                                                  >  8:199748/1‑149 (MQ=255)
                                                                                       gTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGc                                                              <  7:199748/148‑1 (MQ=255)
                                                                                        tCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCg                                                            <  3:99899/149‑1 (MQ=255)
                                                                                          ggtgcggtgGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCgg                                                           <  5:188219/148‑1 (MQ=255)
                                                                                            tgcggtgGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCct                                                        >  2:278284/1‑149 (MQ=255)
                                                                                                 tgGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTcg                                                   >  5:126252/1‑149 (MQ=255)
                                                                                                   gcggcgGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTcgcg                                                 <  3:320739/149‑1 (MQ=255)
                                                                                                                       ttCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGc                             >  7:79337/1‑149 (MQ=255)
                                                                                                                       ttCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGc                             >  1:257082/1‑149 (MQ=255)
                                                                                                                                 cTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGAt                    <  1:107971/148‑1 (MQ=255)
                                                                                                                                    ttCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATcccc                >  2:91681/1‑149 (MQ=255)
                                                                                                                                            ggcTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAg        >  2:362408/1‑149 (MQ=255)
                                                                                                                                                gCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCggg    >  6:182511/1‑149 (MQ=255)
                                                                                                                                                 cGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCggg    <  5:236794/148‑1 (MQ=255)
                                                                                                                                                 cGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCgg     <  2:257082/147‑1 (MQ=255)
                                                                                                                                                  ggCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGtt  >  6:305245/1‑149 (MQ=255)
                                                                                                                                                  ggCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGtt  <  1:91681/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACCAAGTGTCGGCGGCTAACCGCGGGGCGGCGGTGGGGGCGTATTCGCTGTTCATCGACTTGTCACTGGGGGTGACCGGGCCATTGGTCGGTGCGGTGGCGGCGGGGTTCGGCTTTGCTTCGATGTTTCTGTTCGCGGCGGCTGCGGCAGCGTGCGGGTTGGTGTTGAGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTT  >  NC_002947/5104753‑5105047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑