Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,130,433 A→C 8.1% L324R (CTG→CGG)  recQ ← ATP‑dependent DNA 3'‑5' helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,130,4330AC 8.1% 201.7 / 4.1 68L324R (CTG→CGG) recQATP‑dependent DNA 3'‑5' helicase
Reads supporting (aligned to +/- strand):  ref base A (27/35);  new base C (4/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTCTTCGCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGCCCTTCCTCGTTCA  >  NC_002947/5130292‑5130580
                                                                                                                                             |                                                                                                                                                   
gTTTCTTCGCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGt                                                                                                                                               >  1:133638/1‑148 (MQ=255)
 tttCTTCGCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCa                                                                                                                                             <  7:62221/149‑1 (MQ=255)
     ttcGCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCCGGCCGTGAcggc                                                                                                                                         >  4:57805/1‑149 (MQ=255)
     ttcGCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCAcggc                                                                                                                                         >  5:372201/1‑149 (MQ=255)
        gCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCCGGCCGTGAcggcctg                                                                                                                                      >  1:205924/1‑147 (MQ=255)
        gCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCAcggccgg                                                                                                                                      >  5:278285/1‑149 (MQ=255)
          acaGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCCGGCCGTGACggcctgcc                                                                                                                                    <  3:57805/149‑1 (MQ=255)
          acaGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACggccggcc                                                                                                                                    >  5:96514/1‑149 (MQ=255)
          acaGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACggccggcc                                                                                                                                    >  7:213485/1‑149 (MQ=255)
            aGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCCGGCCGTGACGGcctgccct                                                                                                                                  <  2:205924/149‑2 (MQ=255)
                 cAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCccggccgg                                                                                                                             >  1:99132/1‑149 (MQ=255)
                     aTGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTc                                                                                                                         <  3:111717/149‑1 (MQ=255)
                        gCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCCTCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTg                                                                                                                      <  3:221799/149‑1 (MQ=255)
                        gCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCt                                                                                                                       <  5:107376/148‑1 (MQ=255)
                         cGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGa                                                                                                                     <  4:334998/149‑1 (MQ=255)
                           tCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGAta                                                                                                                   <  1:161037/149‑1 (MQ=255)
                             cAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGAtac                                                                                                                  <  8:221420/148‑2 (MQ=255)
                                   gtgCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTc                                                                                                                         <  3:366976/135‑1 (MQ=255)
                                   gtgCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTc                                                                                                                         >  4:366976/1‑135 (MQ=255)
                                    tgctgcTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCtt                                                                                                           <  2:99132/148‑1 (MQ=255)
                                         ctCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGc                                                                                                      <  2:133638/148‑1 (MQ=255)
                                             aTGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGAttt                                                                                                 >  8:274951/1‑149 (MQ=255)
                                              tGCGCTTGTGGCGCTCGTCGCCTGCGGAGTTCGGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTg                                                                                                <  8:152325/149‑1 (MQ=255)
                                               gagcTTGTGGCGCTCGTCGCCTTCGGTGTTCTTCAGGATCTGCTTGAGCATGACCATGTCCTGGAGCCCGTAGGCCATCCAGGCATCGGATGGCAGGCCGTCACGGCCGGCCCTGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTg                                                                                                <  5:362394/146‑1 (MQ=255)
                                                   ttGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGTAg                                                                                           >  4:269727/1‑149 (MQ=255)
                                                     gtgGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAg                                                                                           <  2:251605/147‑1 (MQ=255)
                                                     gtgGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGACATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGt                                                                                         >  1:9458/1‑149 (MQ=255)
                                                         cgcTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGTAGGTCGAg                                                                                     <  3:269727/149‑1 (MQ=255)
                                                          gcTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCCGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAgg                                                                                    >  3:291645/1‑149 (MQ=255)
                                                             cgtcgCCTTCGGCGTTCTTCAGCATCTGCTTTAGCATCACCATGTCCTGCACCCCGTAGGCCATCCATGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGa                                                                                 <  8:302630/149‑1 (MQ=255)
                                                                    ttCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACg                                                                           >  5:289146/1‑148 (MQ=255)
                                                                    ttCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGAATCGGAGGGCCGGCCGTCACGGCCGGCCCGGCCGGTTTCCGGATAGTAGGCTTCAAGCGATTTGGGCGGGTCACGGTGAGGAACg                                                                           >  8:260883/1‑148 (MQ=255)
                                                                       ggAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGa                                                                                                                     <  4:255202/103‑1 (MQ=255)
                                                                       ggAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGa                                                                                 <  4:172527/139‑1 (MQ=255)
                                                                       ggAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGa                                                                                 >  3:172527/1‑139 (MQ=255)
                                                                       ggAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAgc                                                                       <  4:237630/149‑1 (MQ=255)
                                                                               gcagcaTCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTg                                                               >  6:345521/1‑149 (MQ=255)
                                                                                cagcaTCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGtt                                                                <  6:96514/147‑1 (MQ=255)
                                                                                cagcaTCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTgg                                                              <  6:289146/149‑1 (MQ=255)
                                                                                 agcaTCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTggg                                                             <  4:291645/149‑1 (MQ=255)
                                                                                  gcaTCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGc                                                            <  2:127009/149‑1 (MQ=255)
                                                                                        gCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTc                                                       >  6:190475/1‑148 (MQ=255)
                                                                                        gCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTc                                                       >  6:352476/1‑148 (MQ=255)
                                                                                         cTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGt                                                        <  3:203352/146‑1 (MQ=255)
                                                                                         cTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGa                                                     >  7:188829/1‑149 (MQ=255)
                                                                                          ttGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGa                                                     >  8:89938/1‑148 (MQ=255)
                                                                                            gAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATa                                                   <  8:213485/148‑1 (MQ=255)
                                                                                              gCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATAccc                                                <  5:345521/149‑1 (MQ=255)
                                                                                                aTCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGTAGGTCGAGGTGAGCAACGAAGCGCCCGTTGGGCTTGTCGAGACCCag                                              >  6:211777/1‑148 (MQ=255)
                                                                                                  cACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGc                                            >  4:101449/1‑149 (MQ=255)
                                                                                                     cATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCAAGCCg                                          >  8:316228/1‑148 (MQ=255)
                                                                                                         tCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAg                                      <  7:260883/148‑1 (MQ=255)
                                                                                                         tCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAg                                      <  7:89938/148‑1 (MQ=255)
                                                                                                          ccTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCTTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGATGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGc                                     <  7:328483/148‑1 (MQ=255)
                                                                                                           cTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGGGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCaa                                   >  4:89974/1‑149 (MQ=255)
                                                                                                             gCAGCCCGTAGGCCATCCAGGCATCGGAGTGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGa                                                     <  2:218655/129‑1 (MQ=255)
                                                                                                             gCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGa                                                     >  1:218655/1‑129 (MQ=255)
                                                                                                              cAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAAt                                  <  6:278285/147‑1 (MQ=255)
                                                                                                                 cccGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGt                                                        <  7:29675/122‑1 (MQ=255)
                                                                                                                 cccGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGt                                                        >  8:29675/1‑122 (MQ=255)
                                                                                                                      aGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGAc                         >  5:120179/1‑148 (MQ=255)
                                                                                                                              cAGGCATCGGACGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAg                <  2:306110/149‑1 (MQ=255)
                                                                                                                                ggCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGcc              >  5:120663/1‑149 (MQ=255)
                                                                                                                                 gCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGccc             >  2:237595/1‑149 (MQ=255)
                                                                                                                                    tCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGCCCTTc          <  8:188829/149‑1 (MQ=255)
                                                                                                                                         gggCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGCCCTTcc         >  7:231834/1‑145 (MQ=255)
                                                                                                                                         gggCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGCCCTTcc         <  8:231834/145‑1 (MQ=255)
                                                                                                                                            cAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGCCCTTCCTCGTTCa  <  7:274951/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GTTTCTTCGCACAGCGCCAGCATGGCGTCCAGCTTGTGCTGCTCGATGCGCTTGTGGCGCTCGTCGCCTTCGGAGTTCTGCAGCATCTGCTTGAGCATCACCATGTCCTGCAGCCCGTAGGCCATCCAGGCATCGGAGGGCAGGCCGTCACGGCCGGCCCGGCCGGTTTCCTGATAGTAGGCTTCAAGCGATTTGGGCAGGTCGAGGTGAGCAACGAAGCGCACGTTGGGCTTGTCGATACCCATGCCGAAAGCAATGGTAGCGACCATGATCAGCCCTTCCTCGTTCA  >  NC_002947/5130292‑5130580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑