Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,147,421 G→A 100% G176S (GGT→AGT)  mscMB → miniconductance mechanosensitive channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,147,4210GA100.0% 215.8 / NA 64G176S (GGT→AGT) mscMBminiconductance mechanosensitive channel protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/28);  total (36/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACATCTATGCCCGCACCGAACACGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGGGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATGGCGACGTGGTG  >  NC_002947/5147275‑5147564
                                                                                                                                                  |                                                                                                                                               
aCATCTATGCCCGCACCGAACACGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGt                                                                                                                                               >  6:156697/1‑149 (MQ=255)
      aTGCCCGCACCGAACACGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATg                                                                                                                                         >  3:127033/1‑149 (MQ=255)
         cccGCACCGAACACCCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTc                                                                                                                                       <  7:335671/148‑1 (MQ=255)
             cACCGAACACGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACTCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTcggcgg                                                                                                                                  <  1:103272/149‑1 (MQ=255)
                cGAACACGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTAt                                                                                                                               >  7:81432/1‑149 (MQ=255)
                   acacGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCTATGTCGGCGGTAtt                                                                                                                              <  8:81432/147‑1 (MQ=255)
                     acGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTg                                                                                                                          <  5:18312/149‑1 (MQ=255)
                      cGCCCGCACCCGCTCGATCAAGGGCTACGTGCTACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGt                                                                                                                         <  1:18582/149‑1 (MQ=255)
                       gCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGtt                                                                                                                        >  4:372994/1‑149 (MQ=255)
                       gCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGACAAGATGATGTTGTGGATCTTAGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGACTGAGTGCGATGTCGGCGGTATTGCTGtt                                                                                                                        >  1:358885/1‑149 (MQ=255)
                        cccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTcggcg                                                                                                                                   >  5:119092/1‑137 (MQ=255)
                        cccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTcggcg                                                                                                                                   <  6:119092/137‑1 (MQ=255)
                        cccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTg                                                                                                                       <  3:372994/149‑1 (MQ=255)
                        cccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTg                                                                                                                       <  8:204477/149‑1 (MQ=255)
                         ccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTgg                                                                                                                      >  2:246436/1‑149 (MQ=255)
                         ccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTgg                                                                                                                      >  7:273267/1‑149 (MQ=255)
                         ccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTgg                                                                                                                      >  4:338184/1‑149 (MQ=255)
                         ccGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGATGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTgg                                                                                                                      >  1:270120/1‑149 (MQ=255)
                                 gCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAg                                                                                                              <  5:156697/149‑1 (MQ=255)
                                     gATCAAGGGCTACGTGCAACTGGCCAATATTATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTTTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCTGTATTGCTGTTGGTGTACAAGGac                                                                                                           <  2:358885/148‑1 (MQ=255)
                                     gATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGc                                                                                                                                             >  2:37723/1‑114 (MQ=255)
                                     gATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGc                                                                                                                                             <  1:37723/114‑1 (MQ=255)
                                     gATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGaca                                                                                                          >  1:111449/1‑149 (MQ=255)
                                     gATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGaca                                                                                                          >  2:29070/1‑149 (MQ=255)
                                       tCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGtt                                                                                                                        >  6:378934/1‑133 (MQ=255)
                                       tCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGtt                                                                                                                        <  5:378934/133‑1 (MQ=255)
                                        cAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACAccc                                                                                                       >  8:56012/1‑149 (MQ=255)
                                                   tGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCATCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATtc                                                                                            <  4:106517/149‑1 (MQ=255)
                                                      aaCTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATtcgtc                                                                                         <  4:127033/149‑1 (MQ=255)
                                                         tGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGc                                                                                       >  7:86211/1‑148 (MQ=255)
                                                           gCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAg                                                                                    >  3:275151/1‑149 (MQ=255)
                                                              aaGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGt                                                                                 <  4:367826/149‑1 (MQ=255)
                                                              aaGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGt                                                                                 <  1:246436/149‑1 (MQ=255)
                                                                         gtgGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGAcc                                                                       <  2:111449/148‑1 (MQ=255)
                                                                          tgGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCa                                                                      <  7:56012/148‑1 (MQ=255)
                                                                           ggATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAg                                                                     >  2:103909/1‑148 (MQ=255)
                                                                              tCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAAc                                                                 >  5:137376/1‑149 (MQ=255)
                                                                                  cGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCaa                                                                  <  3:338184/144‑1 (MQ=255)
                                                                                    ccTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGa                                                                                                            <  6:243424/100‑1 (MQ=255)
                                                                                    ccTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGa                                                                                                            >  5:243424/1‑100 (MQ=255)
                                                                                    ccTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACAtg                                                           >  7:164491/1‑149 (MQ=255)
                                                                                        gATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACAtgct                                                         <  4:275151/147‑1 (MQ=255)
                                                                                             tcatcGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTc                                                  >  3:335801/1‑149 (MQ=255)
                                                                                              catcGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACAtgctgc                                                       >  3:12121/1‑143 (MQ=255)
                                                                                              catcGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACAtgctgc                                                       <  4:12121/143‑1 (MQ=255)
                                                                                               atcGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCgg                                                >  6:141255/1‑149 (MQ=255)
                                                                                                tcgtcgCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGCCACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCggg                                               >  5:174683/1‑149 (MQ=255)
                                                                                                tcgtcgCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCggg                                               >  5:289138/1‑149 (MQ=255)
                                                                                                  gtcgCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCAGCATGTCgttgc                                             <  6:137376/149‑5 (MQ=255)
                                                                                                     gCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTg                                          >  5:190604/1‑149 (MQ=255)
                                                                                                        aCCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGAt                                       >  7:129955/1‑149 (MQ=255)
                                                                                                        aCCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGAt                                       >  4:261572/1‑149 (MQ=255)
                                                                                                              aTCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGaa                                   <  1:65183/147‑1 (MQ=255)
                                                                                                                  aCCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGACg                             >  6:352634/1‑149 (MQ=255)
                                                                                                                              tGTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGcc                 >  6:115709/1‑149 (MQ=255)
                                                                                                                               gTTGCTGCTGTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGcc                 >  4:275417/1‑148 (MQ=255)
                                                                                                                                   ctgctgTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATg             <  1:26587/148‑1 (MQ=255)
                                                                                                                                     gctgTCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATGGc           >  7:297406/1‑148 (MQ=255)
                                                                                                                                         tCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCg                                                 <  7:271301/106‑1 (MQ=255)
                                                                                                                                         tCCGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCg                                                 >  8:271301/1‑106 (MQ=255)
                                                                                                                                           cGGTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATGGCGACgtgg    <  8:86211/149‑1 (MQ=255)
                                                                                                                                            ggTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATGGCGACgtggt   >  2:371242/1‑149 (MQ=255)
                                                                                                                                            ggTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACACGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATGGCGACgtggt   >  3:354355/1‑149 (MQ=255)
                                                                                                                                             gTCTGAGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATGGCGACgtggtg  <  6:289138/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACATCTATGCCCGCACCGAACACGCCCGCACCCGCTCGATCAAGGGCTACGTGCAACTGGCCAAGATGATGTTGTGGATCTTCGCCTCGATCATCATCGTCGCCACCCTGATCGACCGCTCGCCGCTGTTGCTGCTGTCCGGTCTGGGTGCGATGTCGGCGGTATTGCTGTTGGTGTACAAGGACACCCTGCTGTCATTCGTCGCCAGCGTGCAACTGACCAGCAACGACATGCTGCATGTCGGGGACTGGATCGAAATGCCGCAGGTAGGCGCCGATGGCGACGTGGTG  >  NC_002947/5147275‑5147564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑