Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,156,328 A→G 6.4% S263G (AGC→GGC)  PP_4539 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,156,3280AG 6.4% 183.9 / 2.4 64S263G (AGC→GGC) PP_4539LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (26/33);  new base G (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGA  >  NC_002947/5156187‑5156470
                                                                                                                                             |                                                                                                                                              
cagcGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAgccgcc                                                                                                                                          <  8:321526/148‑1 (MQ=255)
       ggACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTg                                                                                                                                  >  5:350162/1‑149 (MQ=255)
       ggACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTg                                                                                                                                  >  5:344508/1‑149 (MQ=255)
               ggCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCt                                                                                                                          <  4:355140/149‑1 (MQ=255)
                   gCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccc                                                                                                                                      <  3:94993/133‑1 (MQ=255)
                   gCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccc                                                                                                                                      >  4:94993/1‑133 (MQ=255)
                   gCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTAt                                                                                                                      <  5:117123/149‑1 (MQ=255)
                     cTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                    >  6:318833/1‑149 (MQ=255)
                     cTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                    <  2:171017/149‑1 (MQ=255)
                       gggCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                    >  6:42429/1‑147 (MQ=255)
                       gggCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTCTTCTACCTTTATTc                                                                                                                    >  7:321619/1‑147 (MQ=255)
                        ggCACCGCCGCCTCCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAg                                                                                                                 <  1:129580/149‑1 (MQ=255)
                        ggCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGa                                                                                                                  <  6:144381/148‑1 (MQ=255)
                         gCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAg                                                                                                                 >  3:57381/1‑148 (MQ=255)
                         gCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGGGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGc                                                                                                                <  2:133136/149‑1 (MQ=255)
                           aCCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCa                                                                                                               >  2:87730/1‑148 (MQ=255)
                           aCCGCCGCCACCTCCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGTGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCa                                                                                                               >  3:348951/1‑148 (MQ=255)
                            ccgccgCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCaaa                                                                                                             <  4:137267/149‑1 (MQ=255)
                            ccgccgCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCaaa                                                                                                             <  4:131672/149‑1 (MQ=255)
                             cgccgcCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCaaa                                                                                                             <  6:18995/148‑1 (MQ=255)
                             cgccgcCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCa                                                                                                               >  5:18995/1‑146 (MQ=255)
                              gccgccACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAaga                                                                                                           <  6:122896/149‑1 (MQ=255)
                                     ccTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGAcc                                                                                                    >  4:78727/1‑149 (MQ=255)
                                      cTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGAccc                                                                                                   >  5:227552/1‑149 (MQ=255)
                                         cGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTg                                                                                                 <  2:31707/148‑1 (MQ=255)
                                              gcagcGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGagaa                                                                                                        >  8:280105/1‑136 (MQ=255)
                                              gcagcGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGagaa                                                                                                        <  7:280105/136‑1 (MQ=255)
                                                agcGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGc                                                                                         <  4:129625/149‑1 (MQ=255)
                                                   ggCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCAc                                                                                      <  6:11180/149‑1 (MQ=255)
                                                     ccTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACg                                                                                     >  5:102731/1‑148 (MQ=255)
                                                        gggCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACg                                                                                     >  3:192233/1‑145 (MQ=255)
                                                           cTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATtc                                                                              <  3:351675/149‑1 (MQ=255)
                                                           cTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATtc                                                                              <  6:243569/149‑1 (MQ=255)
                                                              tGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATtctc                                                                            <  5:42429/148‑1 (MQ=255)
                                                                ccTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATc                                                                         <  8:334856/149‑1 (MQ=255)
                                                                 cTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCGATTGCCGCTGGATCAGGGTGGCTGCCGCCGCCCGCTGTTCTAGGTGTATTCGAGGAAAGAGAAGCCCCTGGGCCCGGCCACGCTGATTCTCATc                                                                         >  4:86113/1‑148 (MQ=255)
                                                                 cTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCgg                                                                                          <  5:322280/131‑1 (MQ=255)
                                                                 cTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCgg                                                                                          >  6:322280/1‑131 (MQ=255)
                                                                          ccGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCtgctgc                                                               >  6:135798/1‑149 (MQ=255)
                                                                                  tGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                       <  1:356213/149‑1 (MQ=255)
                                                                                  tGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                       <  4:57381/149‑1 (MQ=255)
                                                                                   gATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                       >  3:331641/1‑148 (MQ=255)
                                                                                    aTCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGa                                                     <  4:331641/149‑1 (MQ=255)
                                                                                     tCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGa                                                     >  7:66765/1‑148 (MQ=255)
                                                                                               gCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCAc                                          <  6:227552/149‑1 (MQ=255)
                                                                                                   cgcgAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTg                                        <  5:135798/147‑1 (MQ=255)
                                                                                                   cgcgAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGa                                      >  1:378282/1‑149 (MQ=255)
                                                                                                    gcgAAGGCGTGCTGAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGAc                                     <  4:192233/149‑1 (MQ=255)
                                                                                                     cgAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACg                                    >  7:317528/1‑149 (MQ=255)
                                                                                                               cTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCg                           >  6:86267/1‑148 (MQ=255)
                                                                                                                tCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCg                           >  1:380651/1‑147 (MQ=255)
                                                                                                                  aaGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCa                         <  3:204842/147‑1 (MQ=255)
                                                                                                                  aaGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGc                       >  4:121525/1‑149 (MQ=255)
                                                                                                                  aaGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGATCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGATGGCAAATTTCGACACCGCGCCACTGGACGTGCAATTCGCAGc                       >  7:4382/1‑149 (MQ=255)
                                                                                                                            cGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACaa             <  5:86267/149‑1 (MQ=255)
                                                                                                                            cGCTGGATCAGCGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACaa             <  4:348951/149‑1 (MQ=255)
                                                                                                                                   tCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGa                                                                                 >  3:77851/1‑74 (MQ=255)
                                                                                                                                   tCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGa                                                                                 <  4:77851/74‑1 (MQ=255)
                                                                                                                                   tCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCt         >  7:314977/1‑146 (MQ=255)
                                                                                                                                    gtgggTGGCGGCTGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTc                            <  7:287690/124‑1 (MQ=255)
                                                                                                                                    gtgggTGGCGGCTGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTc                            >  8:287690/3‑126 (MQ=255)
                                                                                                                                     aGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGTCACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGagag    <  1:87730/149‑1 (MQ=255)
                                                                                                                                       ggcggCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGa  <  6:350162/146‑1 (MQ=255)
                                                                                                                                       ggTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGa  <  6:344508/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
CAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGA  >  NC_002947/5156187‑5156470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑