Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	464,320	G→C	8.5%	H259Q (CAC→CAG) ‡	davB ←	lysine 2‑monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	464,320	0	G	C	8.5%	231.0 / 12.2	82	H259Q (CAC→CAG) ‡	davB	lysine 2‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (35/39);  new base C (2/5);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-02

CGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAG  >  NC_002947/464178‑464467                                                                                                                                               |                                                                                                                                                    cgcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtgg                                                                                                                                               <  7:125830/149‑1 (MQ=255)  gcgGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCtggt                                                                                                                                              >  5:83199/1‑149 (MQ=255)       cAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTAGTGgtcg                                                                                                                                         <  8:115309/149‑1 (MQ=255)       cAATGCGCTTGACCCCGGGACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcg                                                                                                                                         <  8:206554/149‑1 (MQ=255)        aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTGGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgg                                                                                                                                        <  2:234739/149‑2 (MQ=255)        aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgg                                                                                                                                        <  5:340788/149‑2 (MQ=255)        aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgg                                                                                                                                        <  4:318822/149‑2 (MQ=255)         aTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGgtcgtc                                                                                                                                       >  8:295264/1‑149 (MQ=255)            cgcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCTCCGACCACCAGGTGCTGGTGGTCGTCGCa                                                                                                                                    <  4:145792/149‑1 (MQ=255)            cgcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCa                                                                                                                                    <  1:239415/149‑1 (MQ=255)             gcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAg                                                                                                                                   >  8:316077/1‑149 (MQ=255)              cTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGt                                                                                                                                  <  3:43899/149‑1 (MQ=255)                 gACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGtt                                                                                                                                 <  1:23855/147‑1 (MQ=255)                  aCCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCATCTGGCAAATGCACGCAGCGTTCCGGTACGACCCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCCCCACCCAGTTGCTGGTGGTCGTCGCAGTTgg                                                                                                                               >  6:325040/1‑148 (MQ=255)                      cGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtcatc                                                                                                                          <  1:379568/149‑1 (MQ=255)                      cGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGtcatc                                                                                                                          <  6:40656/149‑1 (MQ=255)                            gcgGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCAcg                                                                                                                    >  3:300591/1‑149 (MQ=255)                                    ccgccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGCCACATGTACCACGCCCCCGACCACCCGGTGCTGGTGGTCGACGCTGTAGGTCATCACCCCTCGGAAGAt                                                                                                            >  8:327284/1‑149 (MQ=255)                                     cgccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGAtt                                                                                                           >  4:288379/1‑149 (MQ=255)                                        cATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                         >  5:15940/1‑148 (MQ=255)                                        cATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                         >  6:145481/1‑148 (MQ=255)                                         aTGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                         >  4:355136/1‑147 (MQ=255)                                              gCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATc                                                                                                  >  6:157848/1‑149 (MQ=255)                                               cGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCATTTGGTCATCACCACGCGGAAGATTTCCAGCATc                                                                                                  <  2:192641/148‑1 (MQ=255)                                                gTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGa                                                                                                <  5:380332/149‑1 (MQ=255)                                                   cTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGc                                                                                                     <  5:230286/141‑1 (MQ=255)                                                   cTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGc                                                                                                     >  6:230286/1‑141 (MQ=255)                                                   cTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGtt                                                                                             <  7:259263/149‑1 (MQ=255)                                                     cAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTgg                                                                                           >  7:132163/1‑149 (MQ=255)                                                      aGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCa                                                                                                                                    <  8:40104/107‑1 (MQ=255)                                                      aGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCa                                                                                                                                    >  7:40104/1‑107 (MQ=255)                                                         cTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGaa                                                                                       <  4:300591/149‑1 (MQ=255)                                                             tGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTcc                                                                                   <  3:288379/149‑1 (MQ=255)                                                             tGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTcc                                                                                   <  1:225051/149‑1 (MQ=255)                                                                  tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                         >  3:73063/1‑122 (MQ=255)                                                                  tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                         <  4:73063/122‑1 (MQ=255)                                                                  tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                              >  1:366829/1‑149 (MQ=255)                                                                      gCCAATGCACGCAGCGATCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCTAGTCCCaa                                                                          >  6:105860/1‑149 (MQ=255)                                                                        cAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCTAGTTGGGTAAGTCCGAGTCCCAAcc                                                                        <  8:96906/149‑1 (MQ=255)                                                                        cAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAcc                                                                        >  5:250887/1‑149 (MQ=255)                                                                        cAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAcc                                                                        >  1:287543/1‑149 (MQ=255)                                                                              aCGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                         <  5:245101/142‑1 (MQ=255)                                                                              aCGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAc                                                                         >  6:245101/1‑142 (MQ=255)                                                                               cGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGt                                                                  <  2:108227/148‑1 (MQ=255)                                                                                gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTcc                                                                                   <  6:299814/130‑1 (MQ=255)                                                                                gCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCAGCGAGTTGGGGAAGTCCGAGTCCCACCCGCCGGTGc                                                                >  6:295540/1‑149 (MQ=255)                                                                                  aGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                >  5:290792/1‑147 (MQ=255)                                                                                  aGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGc                                                                <  6:290792/147‑1 (MQ=255)                                                                                         cGGTACGTCGCGCCAGATGCCTTGTGACACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGa                                                       >  1:201996/1‑149 (MQ=255)                                                                                               gTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGc                                                 <  7:295264/149‑1 (MQ=255)                                                                                                  gcgcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCg                                               >  1:341096/1‑148 (MQ=255)                                                                                                   cgcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGaa                                             <  3:355136/149‑1 (MQ=255)                                                                                                   cgcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGaa                                             <  4:260225/149‑1 (MQ=255)                                                                                                            ccTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcgcg                                    <  2:287543/149‑1 (MQ=255)                                                                                                            ccTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcgcg                                    <  7:316077/149‑1 (MQ=255)                                                                                                            ccTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTcgcg                                    <  5:105860/149‑1 (MQ=255)                                                                                                             cTTGTGGCACTTGTTCCTCGCCCGGGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTGCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTg                                                   <  4:78251/133‑1 (MQ=255)                                                                                                             cTTGTGGCACTTGTTCCACGCCCCCGACCCCCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCgg                                   <  8:132163/149‑1 (MQ=255)                                                                                                             cTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTg                                                   >  3:78251/1‑133 (MQ=255)                                                                                                             cTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCgg                                   <  5:145481/149‑1 (MQ=255)                                                                                                             cTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGACCCAACACTTcgcgc                                   >  7:31357/1‑148 (MQ=255)                                                                                                                gtgGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGc                                >  5:37592/1‑149 (MQ=255)                                                                                                                 tgGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGc                                <  5:325040/148‑1 (MQ=255)                                                                                                                   gCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTgg                             <  2:366829/149‑1 (MQ=255)                                                                                                                     aCTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCaa                                                                          >  1:153871/1‑102 (MQ=255)                                                                                                                     aCTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCaa                                                                          <  2:153871/102‑1 (MQ=255)                                                                                                                     aCTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTgg                             >  6:334312/1‑147 (MQ=255)                                                                                                                      cTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAg                          <  6:15940/149‑1 (MQ=255)                                                                                                                       ttGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGc                         >  8:237162/1‑149 (MQ=255)                                                                                                                       ttGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGc                         >  3:327154/1‑149 (MQ=255)                                                                                                                          ttCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCTTCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCTGTGCTGGAAGCTCa                      <  6:250887/149‑1 (MQ=255)                                                                                                                          ttCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTc                       <  6:323401/148‑1 (MQ=255)                                                                                                                          ttCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCCGTGCCGAAACCCACCAGGCCCAACACTTCCCCGTGCTGGAAGCt                        >  6:60515/1‑147 (MQ=255)                                                                                                                             cACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCt                   >  3:118609/1‑149 (MQ=255)                                                                                                                              actgcgacGACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt                  >  7:79526/8‑149 (MQ=255)                                                                                                                                gCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTgg                <  6:83199/149‑1 (MQ=255)                                                                                                                                    ccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa             >  3:190536/1‑148 (MQ=255)                                                                                                                                    acGACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaa             >  7:354353/2‑148 (MQ=255)                                                                                                                                       accaccAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAaga         <  5:60515/149‑1 (MQ=255)                                                                                                                                       accaccAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAaga         <  7:237162/149‑1 (MQ=255)                                                                                                                                         caccaGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAgc       >  2:278003/1‑149 (MQ=255)                                                                                                                                          accaGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAgcg      >  1:100118/1‑149 (MQ=255)                                                                                                                                              ggTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAg  <  5:125611/149‑1 (MQ=255)                                                                                                                                              ggTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAg  >  6:125611/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                    CGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGAGCGCGAG  >  NC_002947/464178‑464467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑