| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,192,759 | T→G | 13.3% | intergenic (+140/‑62) | PP_4572 → / → PP_4573 | hypothetical protein/AAA family ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,192,759 | 0 | T | G | 13.3% | 104.5 / 7.1 | 44 | intergenic (+140/‑62) | PP_4572/PP_4573 | hypothetical protein/AAA family ATPase |
| Reads supporting (aligned to +/- strand): ref base T (22/16); new base G (3/3); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01 | |||||||||||
ACTGAGACAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGC > NC_002947/5192615‑5192905 | actgaGACAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCttatt < 4:115932/149‑1 (MQ=255) tgaGACAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAg > 3:258245/1‑149 (MQ=255) aCAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTC‑CAACCTTATTAGgcgc > 2:71360/1‑149 (MQ=255) aGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCgg < 8:264645/149‑1 (MQ=255) gTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCAGCCCCTA‑CAACCTTATTAGGCGCGGGtt > 2:238076/1‑149 (MQ=255) aCGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCGCCTA‑ATAAGGTTGTAGGGGCGGGTTTAc < 8:105621/149‑1 (MQ=25) ggggCACTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCGCCTA‑ATAAGGTTGTAGGGGCGGGTTTAccc < 3:224070/149‑1 (MQ=25) aTTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATCGGCGCAAACAAATCCAGATCATGGGCTGCCTGTTCTGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGaa < 1:298491/149‑1 (MQ=38) ttCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCcgcg > 7:356052/1‑146 (MQ=255) cAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAaga < 6:372130/148‑1 (MQ=255) gAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAAGCTTATTTGGCGCGGGTTTACCCGCGAAGAGGCCCGCa > 3:244508/1‑148 (MQ=255) aTACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCGA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAg > 4:97425/1‑149 (MQ=255) ccGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGccc > 5:300313/1‑148 (MQ=255) gTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCt < 4:336595/146‑1 (MQ=255) gTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCGTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTaag > 3:336595/1‑148 (MQ=255) gTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAgg > 5:371587/1‑149 (MQ=255) tttGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCGCCTA‑ATAAGGTTGTAGGGGCGGGTTTAccc < 6:350772/117‑1 (MQ=18) tttGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCGCCTA‑ATAAGGTTGTAGGGGCGGGTTTAccc > 5:350772/1‑117 (MQ=18) ttGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCttatt < 2:74227/100‑1 (MQ=255) ttGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCttatt > 1:74227/1‑100 (MQ=255) gccgccAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCAA‑CAACCGTATTTTGGGCGGGTTTACCCGCGAAGAGGGCCCGAATGCCCTAAACAGAAGGCaaaac > 7:313944/1‑147 (MQ=37) gccAGGCCCCGCGTAATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTACGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAAccccc < 5:205623/149‑1 (MQ=255) cgcgTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTc > 1:28465/1‑149 (MQ=255) ttATAGTCGGAAACAAATCCAGATCATGTTCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAg < 1:238076/149‑1 (MQ=255) gTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGc > 7:349433/1‑146 (MQ=255) gTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGc < 8:349433/146‑1 (MQ=255) ggAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCcgc < 6:300313/149‑1 (MQ=255) aTCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGt > 5:103139/1‑149 (MQ=255) ctcctgtgCGGCCTGTGCGGGCATCTTCGGGGGTAAACCGGCGCCTA‑CCACCTTATTAGGCGCGGGATTTCCCGGGAAGAGGCCCGCAAAGCCGTAAACAGAGGACTAACCCCCATGATGTTCGAAGGCACCCGCGACTACGTCTcc < 4:258245/143‑1 (MQ=255) aTCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCca > 3:333042/1‑148 (MQ=255) gtggtgCCTGTTCGGGCCGCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGTTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGAt < 2:85736/148‑1 (MQ=255) gtgCCGCCTCTTGGGGCTTCTTGGCGTGAAAACCCCCCCCTA‑CAACCTTTTTCGGGGGGGGTTAACCCGGTAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGAt < 8:313944/148‑1 (MQ=255) gggCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTgg < 6:72656/148‑1 (MQ=255) ggCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTCGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCg < 4:333042/149‑1 (MQ=255) tctTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTa > 6:127372/1‑148 (MQ=255) ggggTAAACCCCCCCCCACCAACATTTTTCGCCGGGGTTTAACCCGGTAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAAcgcg < 1:371182/148‑1 (MQ=255) cgGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCgg > 4:286061/1‑149 (MQ=255) ccGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTGGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTAccc > 1:353238/1‑149 (MQ=255) ccGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTAccc > 1:347592/1‑149 (MQ=255) cAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTc < 3:138753/142‑1 (MQ=255) cAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTc > 4:138753/1‑142 (MQ=255) aaCCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGccc > 2:59721/1‑149 (MQ=255) aaCCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGccc > 1:153988/1‑149 (MQ=255) aaCCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGacc > 3:301549/1‑149 (MQ=255) ccTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAg > 7:329594/1‑101 (MQ=255) ccTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAg < 8:329594/101‑1 (MQ=255) cTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGccctt > 2:247510/1‑146 (MQ=255) | ACTGAGACAGCGTGACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTA‑CAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGC > NC_002947/5192615‑5192905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |