Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,197,452 T→G 13.8% I378L (ATT→CTT)  PP_4578 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,197,4520TG13.8% 141.2 / 17.5 58I378L (ATT→CTT) PP_4578MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (24/26);  new base G (6/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

GCCTGCAGCTGTTTGCCGCGGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTG  >  NC_002947/5197304‑5197600
                                                                                                                                                    |                                                                                                                                                    
gcCTGCAGCTGTTTGCCGCGGGTTTCAGTCAAGCTCATGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAAGATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAAt                                                                                                                                                      <  6:1400/149‑1 (MQ=255)
   tGCAGCTGTTTGCCGCGGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGAc                                                                                                                                                   >  2:296236/1‑149 (MQ=255)
              gCCGCGGGTTTCAGTCATGCTCAAGGCCGCCACTATCACAATGCCGTAGTACACCTCATAAAACACGCCGATACCCATGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCTGCAATGACCTTTCCGAtg                                                                                                                                         <  4:268611/148‑1 (MQ=255)
                cgcgGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttg                                                                                                                                      <  1:296236/149‑1 (MQ=255)
                 gcgGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttgt                                                                                                                                     <  7:3471/149‑1 (MQ=255)
                     gTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACa                                                                                                                                 <  7:262047/149‑1 (MQ=255)
                         cAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttgt                                                                                                                                     <  7:42920/141‑1 (MQ=255)
                         cAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttgt                                                                                                                                     >  8:42920/1‑141 (MQ=255)
                          aGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCACGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGc                                                                                                                            <  7:282642/149‑1 (MQ=255)
                                  tCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGcccc                                                                                                                    >  4:95046/1‑149 (MQ=255)
                                     aGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccccgaa                                                                                                                 <  7:74322/149‑1 (MQ=255)
                                         cgccACGATCACAATGCAGTAGGACACCGCAGAAAACACGCAGATACCCAGGCCCAGCGGAACATTCTGGCAGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCTAAGTTGTAACAGAAGCAATGccacgaacccc                                                                                                             >  8:65966/1‑149 (MQ=255)
                                                  cacaATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccccgaa                                                                                                                 <  4:2113/136‑1 (MQ=255)
                                                  cacaATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccccgaa                                                                                                                 >  3:2113/1‑136 (MQ=255)
                                                     aaTGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGaa                                                                                                                              >  1:303278/1‑120 (MQ=255)
                                                     aaTGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGaa                                                                                                                              <  2:303278/120‑1 (MQ=255)
                                                      aTGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTggg                                                                                                <  3:62568/149‑1 (MQ=255)
                                                          cGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCCAGCATGCCGATCATCAACGGAAACAGCGCGTCAATGACCTTCCCGATGTTGTAACAGAAGCCCTGACCCGAACCCCGAATGCGCGTGGGGaa                                                                                             >  2:275951/1‑148 (MQ=255)
                                                              ggACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGtt                                                                                        <  2:5973/149‑1 (MQ=255)
                                                                  acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccc                                                                                                                     >  3:46556/1‑116 (MQ=255)
                                                                  acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccc                                                                                                                     <  4:46556/116‑1 (MQ=255)
                                                                  acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                    <  5:159687/149‑1 (MQ=255)
                                                                  acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                    <  1:198374/149‑1 (MQ=255)
                                                                                   gATACCCATGCCCAGCGGAACATTCTGGCCGAGCATGCAGATCCTCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   <  2:308056/149‑1 (MQ=255)
                                                                                   gATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   <  3:95046/149‑1 (MQ=255)
                                                                                        ccAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCTCGGCAATGACCTTGCAGATGTTGTAACAGAAGACCTGCCCCGAACCCCGAATGCGCGTGGGGATCAGTTCGCTCAGAAACGACCCCATGCCGcg                                                               >  8:281175/1‑147 (MQ=255)
                                                                                        ccAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCt                                                               >  7:259609/1‑148 (MQ=255)
                                                                                            gCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGt                                                                                                   >  6:152507/1‑108 (MQ=255)
                                                                                            gCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGt                                                                                                   <  5:152507/108‑1 (MQ=255)
                                                                                             cccAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            <  1:194106/146‑1 (MQ=255)
                                                                                             cccAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            >  2:194106/1‑146 (MQ=255)
                                                                                              ccAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGTGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATa                                                        <  5:133101/149‑1 (MQ=255)
                                                                                               cAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATAc                                                       >  6:268935/1‑149 (MQ=255)
                                                                                                    gAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAg                                                  >  1:270454/1‑149 (MQ=255)
                                                                                                         ttCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   >  1:192134/1‑127 (MQ=255)
                                                                                                         ttCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   <  2:192134/127‑1 (MQ=255)
                                                                                                         ttCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCg                                               >  1:334481/1‑147 (MQ=255)
                                                                                                            tGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCgaag                                            >  8:7031/1‑147 (MQ=255)
                                                                                                             ggCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGaagaag                                         >  5:85683/1‑149 (MQ=255)
                                                                                                                       gCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                    >  8:118995/1‑96 (MQ=255)
                                                                                                                         cGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGGTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAgg                             <  8:225241/149‑1 (MQ=255)
                                                                                                                              atcaACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCa                         <  2:334481/148‑1 (MQ=255)
                                                                                                                               tcaACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGc                       >  3:129255/1‑149 (MQ=255)
                                                                                                                                   cGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCca                     >  4:177814/1‑147 (MQ=255)
                                                                                                                                    ggAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAg                  >  3:335184/1‑149 (MQ=255)
                                                                                                                                     gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGATCAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAg                  >  4:167221/1‑148 (MQ=255)
                                                                                                                                     gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGc                 <  5:268935/149‑1 (MQ=255)
                                                                                                                                     gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGc                 <  8:259609/149‑1 (MQ=255)
                                                                                                                                         aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            <  1:110037/97‑1 (MQ=255)
                                                                                                                                         aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            >  2:110037/6‑102 (MQ=255)
                                                                                                                                         aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGa                                                             >  8:111174/6‑101 (MQ=255)
                                                                                                                                         aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGa                                                             <  7:111174/96‑1 (MQ=255)
                                                                                                                                          aGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCAc            <  2:270454/149‑1 (MQ=255)
                                                                                                                                            atcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGGAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGc          >  3:116967/3‑149 (MQ=255)
                                                                                                                                            atcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCCCAGCATCACGc          >  8:333373/3‑149 (MQ=255)
                                                                                                                                             tcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGc          >  6:152124/2‑148 (MQ=255)
                                                                                                                                              cgGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCa        >  6:47304/1‑149 (MQ=255)
                                                                                                                                               ggCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCAt       >  4:77390/1‑149 (MQ=255)
                                                                                                                                                 cAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCg     <  4:335184/149‑1 (MQ=255)
                                                                                                                                                    tGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCTGTCAGAAACGCCCCCATGCCGCTGAAGATACCCTAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTg  <  1:275951/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCTGCAGCTGTTTGCCGCGGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTG  >  NC_002947/5197304‑5197600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑