Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,197,454 A→T 13.6% V377D (GTC→GAC)  PP_4578 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,197,4540AT13.6% 135.0 / 13.1 59V377D (GTC→GAC) PP_4578MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base T (6/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

TGCAGCTGTTTGCCGCGGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTG  >  NC_002947/5197307‑5197600
                                                                                                                                                   |                                                                                                                                                  
tGCAGCTGTTTGCCGCGGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGAc                                                                                                                                                   >  2:296236/1‑149 (MQ=255)
           gCCGCGGGTTTCAGTCATGCTCAAGGCCGCCACTATCACAATGCCGTAGTACACCTCATAAAACACGCCGATACCCATGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCTGCAATGACCTTTCCGAtg                                                                                                                                         <  4:268611/148‑1 (MQ=255)
             cgcgGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttg                                                                                                                                      <  1:296236/149‑1 (MQ=255)
              gcgGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttgt                                                                                                                                     <  7:3471/149‑1 (MQ=255)
                  gTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACa                                                                                                                                 <  7:262047/149‑1 (MQ=255)
                      cAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttgt                                                                                                                                     >  8:42920/1‑141 (MQ=255)
                      cAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGAtgttgt                                                                                                                                     <  7:42920/141‑1 (MQ=255)
                       aGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCACGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGc                                                                                                                            <  7:282642/149‑1 (MQ=255)
                               tCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGcccc                                                                                                                    >  4:95046/1‑149 (MQ=255)
                                  aGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccccgaa                                                                                                                 <  7:74322/149‑1 (MQ=255)
                                      cgccACGATCACAATGCAGTAGGACACCGCAGAAAACACGCAGATACCCAGGCCCAGCGGAACATTCTGGCAGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCTAAGTTGTAACAGAAGCAATGccacgaacccc                                                                                                             >  8:65966/1‑149 (MQ=255)
                                               cacaATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccccgaa                                                                                                                 <  4:2113/136‑1 (MQ=255)
                                               cacaATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccccgaa                                                                                                                 >  3:2113/1‑136 (MQ=255)
                                                  aaTGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGaa                                                                                                                              >  1:303278/1‑120 (MQ=255)
                                                  aaTGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGaa                                                                                                                              <  2:303278/120‑1 (MQ=255)
                                                   aTGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTggg                                                                                                <  3:62568/149‑1 (MQ=255)
                                                       cGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCCAGCATGCCGATCATCAACGGAAACAGCGCGTCAATGACCTTCCCGATGTTGTAACAGAAGCCCTGACCCGAACCCCGAATGCGCGTGGGGaa                                                                                             >  2:275951/1‑148 (MQ=255)
                                                           ggACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGtt                                                                                        <  2:5973/149‑1 (MQ=255)
                                                               acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccc                                                                                                                     <  4:46556/116‑1 (MQ=255)
                                                               acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGccc                                                                                                                     >  3:46556/1‑116 (MQ=255)
                                                               acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                    <  1:198374/149‑1 (MQ=255)
                                                               acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                    <  5:159687/149‑1 (MQ=255)
                                                                                gATACCCATGCCCAGCGGAACATTCTGGCCGAGCATGCAGATCCTCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   <  2:308056/149‑1 (MQ=255)
                                                                                gATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   <  3:95046/149‑1 (MQ=255)
                                                                                     ccAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCTCGGCAATGACCTTGCAGATGTTGTAACAGAAGACCTGCCCCGAACCCCGAATGCGCGTGGGGATCAGTTCGCTCAGAAACGACCCCATGCCGcg                                                               >  8:281175/1‑147 (MQ=255)
                                                                                     ccAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCt                                                               >  7:259609/1‑148 (MQ=255)
                                                                                         gCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGt                                                                                                   >  6:152507/1‑108 (MQ=255)
                                                                                         gCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGt                                                                                                   <  5:152507/108‑1 (MQ=255)
                                                                                          cccAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            <  1:194106/146‑1 (MQ=255)
                                                                                          cccAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            >  2:194106/1‑146 (MQ=255)
                                                                                           ccAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGTGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATa                                                        <  5:133101/149‑1 (MQ=255)
                                                                                            cAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATAc                                                       >  6:268935/1‑149 (MQ=255)
                                                                                                 gAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAg                                                  >  1:270454/1‑149 (MQ=255)
                                                                                                      ttCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   >  1:192134/1‑127 (MQ=255)
                                                                                                      ttCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                   <  2:192134/127‑1 (MQ=255)
                                                                                                      ttCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCg                                               >  1:334481/1‑147 (MQ=255)
                                                                                                         tGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCgaag                                            >  8:7031/1‑147 (MQ=255)
                                                                                                          ggCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGaagaag                                         >  5:85683/1‑149 (MQ=255)
                                                                                                                    gCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                    >  8:118995/1‑96 (MQ=255)
                                                                                                                      cGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGGTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAgg                             <  8:225241/149‑1 (MQ=255)
                                                                                                                           atcaACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCa                         <  2:334481/148‑1 (MQ=255)
                                                                                                                            tcaACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGc                       >  3:129255/1‑149 (MQ=255)
                                                                                                                                cGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCca                     >  4:177814/1‑147 (MQ=255)
                                                                                                                                 ggAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAg                  >  3:335184/1‑149 (MQ=255)
                                                                                                                                  gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGATCAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAg                  >  4:167221/1‑148 (MQ=255)
                                                                                                                                  gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGc                 <  5:268935/149‑1 (MQ=255)
                                                                                                                                  gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGc                 <  8:259609/149‑1 (MQ=255)
                                                                                                                                      aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            <  1:110037/97‑1 (MQ=255)
                                                                                                                                      aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGaa                                                            >  2:110037/6‑102 (MQ=255)
                                                                                                                                      aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGa                                                             <  7:111174/96‑1 (MQ=255)
                                                                                                                                      aacatcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGa                                                             >  8:111174/6‑101 (MQ=255)
                                                                                                                                       aGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCAc            <  2:270454/149‑1 (MQ=255)
                                                                                                                                         atcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGGAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGc          >  3:116967/3‑149 (MQ=255)
                                                                                                                                         atcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCCCAGCATCACGc          >  8:333373/3‑149 (MQ=255)
                                                                                                                                          tcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGc          >  6:152124/2‑148 (MQ=255)
                                                                                                                                           cgGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCa        >  6:47304/1‑149 (MQ=255)
                                                                                                                                            ggCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCAt       >  4:77390/1‑149 (MQ=255)
                                                                                                                                              cAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCg     <  4:335184/149‑1 (MQ=255)
                                                                                                                                                 tGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCTGTCAGAAACGCCCCCATGCCGCTGAAGATACCCTAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTg  <  1:275951/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCAGCTGTTTGCCGCGGGTTTCAGGCAAGCTCAAGGCCGCCACGATCACAATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTG  >  NC_002947/5197307‑5197600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑