Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,205,616 C→A 5.8% V273L (GTG→TTG)  PP_4586 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,205,6160CA 5.8% 274.0 / 5.7 87V273L (GTG→TTG) PP_4586hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/39);  new base A (3/2);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-02

TTGCTCAGACGCGCCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAA  >  NC_002947/5205471‑5205763
                                                                                                                                                 |                                                                                                                                                   
ttgctCAGACGCGCCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCaa                                                                                                                                                  >  8:87909/1‑149 (MQ=255)
    tCAGACGCGCCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTcg                                                                                                                                              >  3:269818/1‑149 (MQ=255)
      aGACGCGCCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTcgc                                                                                                                                             <  2:126699/148‑1 (MQ=255)
        aCGCGCCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCTAACAGTTGCACCAATTCGCgca                                                                                                                                          <  6:372234/149‑1 (MQ=255)
         cgcgCCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCgcag                                                                                                                                         <  6:244281/149‑1 (MQ=255)
          gcgcCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCgcag                                                                                                                                         >  4:324071/1‑148 (MQ=255)
           cgcCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAATTGGTGCAACTGTTCgcgc                                                                                                                                           <  3:218142/145‑1 (MQ=255)
           cgcCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAATTGGTGCAACTGTTCgcgc                                                                                                                                           >  4:218142/1‑145 (MQ=255)
                   aGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTa                                                                                                                               <  3:125443/149‑1 (MQ=255)
                   aGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGACGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTa                                                                                                                               >  5:374680/1‑149 (MQ=255)
                    gACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTAt                                                                                                                              >  5:79019/1‑149 (MQ=255)
                      ccTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTAtt                                                                                                                             <  5:263431/148‑1 (MQ=255)
                               ggTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCg                                                                                                                   >  1:11006/1‑149 (MQ=255)
                                gTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGc                                                                                                                  >  8:243481/1‑149 (MQ=255)
                                 tcatcaCTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCt                                                                                                                 >  7:68483/1‑149 (MQ=255)
                                   atcaCTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGcc                                                                                                                          <  5:362231/138‑1 (MQ=255)
                                   atcaCTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGcc                                                                                                                          >  6:362231/1‑138 (MQ=255)
                                   atcaCTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGa                                                                                                               >  5:284253/1‑149 (MQ=255)
                                    tcaCTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTGACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGAc                                                                                                              >  5:384206/1‑149 (MQ=255)
                                       cTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCAt                                                                                                           >  1:180617/1‑149 (MQ=255)
                                        tCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACGAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCGATTGCCGTTTTCGCTGACCATg                                                                                                          >  7:239514/1‑149 (MQ=255)
                                        tCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCgcag                                                                                                                                         <  7:306929/118‑1 (MQ=255)
                                        tCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCgcag                                                                                                                                         >  8:306929/1‑118 (MQ=255)
                                        tCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATg                                                                                                          >  8:292018/1‑149 (MQ=255)
                                           tCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGc                                                                                                       <  3:283496/149‑1 (MQ=255)
                                            cccgcccgAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGcc                                                                                                      >  3:228779/1‑149 (MQ=255)
                                              cgcccgAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCgg                                                                                                    <  5:258091/149‑1 (MQ=255)
                                                 ccgAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCa                                                                                                 <  6:284253/149‑1 (MQ=255)
                                                      cggcgTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGtt                                                                                            <  7:87909/149‑1 (MQ=255)
                                                       ggcgTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGtt                                                                                            >  1:319348/1‑148 (MQ=255)
                                                        gcgTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCa                                                                                          <  1:371495/149‑1 (MQ=255)
                                                         cgTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAg                                                                                         <  2:11006/149‑1 (MQ=255)
                                                           tAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGTCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAgg                                                                                        >  5:362714/1‑148 (MQ=255)
                                                                 cTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAAc                                                                                  >  5:355950/1‑148 (MQ=255)
                                                                  tCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAAc                                                                                  >  2:236800/1‑147 (MQ=255)
                                                                  tCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAAc                                                                                  >  4:318487/1‑147 (MQ=255)
                                                                  tCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGa                                                                                >  6:8126/1‑149 (MQ=255)
                                                                    gTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAg                                                                               <  1:124927/148‑1 (MQ=255)
                                                                       ggCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGccc                                                                            <  4:269818/148‑1 (MQ=255)
                                                                        gCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCt                                                                           <  6:374680/148‑1 (MQ=255)
                                                                         cGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTgc                                                                         <  6:384206/149‑1 (MQ=255)
                                                                          gTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTgcg                                                                        >  7:221427/1‑149 (MQ=255)
                                                                           taataaTCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTgcg                                                                        >  7:144303/1‑148 (MQ=255)
                                                                            aataatCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTATTCAGGTACAACGAGCCCTgcg                                                                        >  8:59256/1‑147 (MQ=255)
                                                                             ataatCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACGAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCa                                                                      <  7:243481/148‑1 (MQ=255)
                                                                             ataatCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCa                                                                      <  3:318487/148‑1 (MQ=255)
                                                                                atCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACACCGCGCCCTGCGCAGCCa                                                                  >  6:969/1‑149 (MQ=255)
                                                                                atCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCa                                                                  >  4:352159/1‑149 (MQ=255)
                                                                                   ggTCAGGCTCTCTACCAGTCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATa                                                                <  6:362714/148‑1 (MQ=255)
                                                                                   ggTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATa                                                                <  2:15405/148‑1 (MQ=255)
                                                                                   ggTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATa                                                                <  5:337344/148‑1 (MQ=255)
                                                                                   ggTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAg                                                               <  6:285560/149‑1 (MQ=255)
                                                                                       aGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGccc                                                            <  6:252970/148‑1 (MQ=255)
                                                                                         gCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAg                                                               <  1:375199/143‑1 (MQ=255)
                                                                                         gCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAg                                                               >  2:375199/1‑143 (MQ=255)
                                                                                         gCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTc                                                         <  6:79019/149‑1 (MQ=255)
                                                                                           tctctACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGc                                                       >  2:145974/1‑149 (MQ=255)
                                                                                               tACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCcaca                                                   >  4:62384/1‑149 (MQ=255)
                                                                                                 ccagccagTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATg                                                 <  8:68483/149‑1 (MQ=255)
                                                                                                 ccagccagTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCGATTGCCGTTGTCGCGGCCCAGGGGCCGGGCACGGTTCCGGGACAACGCGCCCTGCGCAGCCATCGCCCCTCGcccccgg                                                 >  8:327342/1‑144 (MQ=255)
                                                                                                    gccagTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCg                                              <  1:145974/149‑1 (MQ=255)
                                                                                                     ccagTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGt                                             >  7:282411/1‑149 (MQ=255)
                                                                                                           aCTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTAATGCCGTTTTCGCTGACCATGGGCCGGGGACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCACTCGCCACATGCCGTCAcgcg                                       >  8:4638/1‑149 (MQ=255)
                                                                                                            cTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTCTTCAGGTACAACGAGCCATCCGCAGCCATAGCCCCTCGCCACATGCCGTCAcgctc                                      >  8:160948/1‑147 (MQ=255)
                                                                                                              gCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACAGGCCGTCCCGCGccc                                    >  6:260931/1‑149 (MQ=255)
                                                                                                                 atCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGa                                 >  3:990/2‑149 (MQ=255)
                                                                                                                    aCGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGAcc                               <  2:180617/148‑1 (MQ=255)
                                                                                                                       ccccTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCaa                             >  3:148378/1‑147 (MQ=255)
                                                                                                                          cTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAagag                          >  8:355434/1‑147 (MQ=255)
                                                                                                                          cTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGagca                        <  7:355434/149‑1 (MQ=255)
                                                                                                                          cTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCGATTGCCGTTTTCTCTGACCATGGGCCGGGCACTGTTCGGGTACAACGAGCCCTGCGCAGCAATAGCCCCTCGCCACAAGCCGTCACGCGCCCCGACCAagagac                        >  4:63731/1‑147 (MQ=255)
                                                                                                                              aTCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCtt                    <  3:62384/149‑1 (MQ=255)
                                                                                                                               tCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTg                   >  1:40938/1‑149 (MQ=255)
                                                                                                                                 tGCGCGAATTGGTGCAACTGTTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTggg                 >  1:198424/1‑149 (MQ=255)
                                                                                                                                  gcgcGAATTGGTGCAACTGTTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCg                                              <  2:370775/119‑1 (MQ=255)
                                                                                                                                  gcgcGAATTGGTGCAACTGTTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCg                                              >  1:370775/1‑119 (MQ=255)
                                                                                                                                   cgcgAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACATCGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTgg                  <  5:260931/146‑1 (MQ=255)
                                                                                                                                   cgcgAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTg                   <  7:160948/145‑1 (MQ=255)
                                                                                                                                    gcgAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTATTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCgg              <  7:59256/149‑1 (MQ=255)
                                                                                                                                     cgAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCggg             <  5:88394/149‑1 (MQ=255)
                                                                                                                                      gAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCcaca                                                   <  2:219265/110‑1 (MQ=255)
                                                                                                                                      gAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCcaca                                                   >  1:219265/1‑110 (MQ=255)
                                                                                                                                      gAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCgtgt            <  2:40938/149‑1 (MQ=255)
                                                                                                                                              gCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCgg     >  5:336793/1‑148 (MQ=255)
                                                                                                                                                aCCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCGGCTTGGGCGGGTTACGCGGGaa  <  8:154654/149‑1 (MQ=255)
                                                                                                                                                aCCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCgg     >  3:220114/1‑146 (MQ=255)
                                                                                                                                                aCCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCgg     <  4:220114/146‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TTGCTCAGACGCGCCTGCCAGACCTGGTAGCGGTCATCACTCATCCCGCCCGAGCGGCGTAACAACTCGTTGGCGTAATAATCGGTCAGGCTCTCTACCAGCCAGTCACTGCCGTCACGCCCCTCAATCTGCGCGAACAGTTGCACCAATTCGCGCAGCAACGGGCTATTGCCGTTTTCGCTGACCATGGGCCGGGCACTGTTCAGGTACAACGAGCCCTGCGCAGCCATAGCCCCTCGCCACATGCCGTCACGCGCCCCGACCAAGAGCAGCTTGGGCGGGTTACGCGGGAA  >  NC_002947/5205471‑5205763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑