Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,225,905 C→A 7.4% P48H (CCT→CAT)  PP_4605 → AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,225,9050CA 7.4% 172.9 / 3.2 54P48H (CCT→CAT) PP_4605AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (23/27);  new base A (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CCGCCCATGACGCCACTCACCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATGGCGCGATCCGCCT  >  NC_002947/5225757‑5226052
                                                                                                                                                    |                                                                                                                                                   
ccGCCCATGACGCCACTCACCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGcc                                                                                                                                                     <  6:335195/149‑1 (MQ=255)
  gCCCATGACGCCACTCACCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTa                                                                                                                                                   <  8:56376/149‑1 (MQ=255)
     cATGACGCCACTCACCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGcc                                                                                                                                                <  2:155862/149‑1 (MQ=255)
       tGACGCCACTCACCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCAt                                                                                                                                              <  2:78069/149‑1 (MQ=255)
          cGCCACTCACCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATca                                                                                                                                            >  8:215907/1‑148 (MQ=255)
                  aCCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGTAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTc                                                                                                                                   <  5:73367/149‑1 (MQ=255)
                  aCCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTg                                                                                                                                     <  3:245776/147‑1 (MQ=255)
                  aCCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTg                                                                                                                                     <  3:144234/147‑1 (MQ=255)
                  aCCCACTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTg                                                                                                                                     <  6:358498/147‑1 (MQ=255)
                    ccAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGt                                                                                                                                 <  2:78862/149‑1 (MQ=255)
                    ccAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGt                                                                                                                                 >  1:345488/1‑149 (MQ=255)
                       gTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCt                                                                                                                              <  1:38337/149‑1 (MQ=255)
                            aaGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATc                                                                                                                         >  4:313134/1‑149 (MQ=255)
                               tgtTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGcc                                                                                                                                                >  5:61254/1‑123 (MQ=255)
                               tgtTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGcc                                                                                                                                                <  6:61254/123‑1 (MQ=255)
                                 ttCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGa                                                                                                                    >  5:239432/1‑149 (MQ=255)
                                  tCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGAc                                                                                                                   >  4:97905/1‑149 (MQ=255)
                                   cAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGAcg                                                                                                                  >  4:257340/1‑149 (MQ=255)
                                          atgcatgcCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGAcggcaccgg                                                                                                           <  4:191882/149‑1 (MQ=255)
                                                   tCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTTGCGGCGGCGTTGTGGAGCAAACGAGACGATTCGCGAGACTACCAGGCGCCTAGCCATCACCCCTTGTCGAGCTACATCGCCGACGGCACAGGCACCTTccg                                                                                                  >  7:245056/1‑149 (MQ=255)
                                                      cccAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTccgccg                                                                                               >  2:321642/1‑149 (MQ=255)
                                                       ccAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCcgccgc                                                                                              <  5:349263/149‑1 (MQ=255)
                                                              cAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGcc                                                                                       >  2:145591/1‑149 (MQ=255)
                                                                 gCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCgg                                                                                    <  7:215907/149‑1 (MQ=255)
                                                                   tGGAGCTGAGCGCGCACCTGGGCGACGGACTGGTGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGcc                                                                                  >  8:330561/1‑149 (MQ=255)
                                                                   tGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGcc                                                                                  >  1:190608/1‑149 (MQ=255)
                                                                       gCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCa                                                                                            <  8:178820/135‑1 (MQ=255)
                                                                       gCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCa                                                                                            >  7:178820/1‑135 (MQ=255)
                                                                       gCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGAc                                                                               >  3:84885/1‑148 (MQ=255)
                                                                       gCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGAc                                                                               >  6:37878/1‑148 (MQ=255)
                                                                       gCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGAc                                                                               >  4:131780/1‑148 (MQ=255)
                                                                           aGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACaaa                                                                            <  1:105402/147‑1 (MQ=255)
                                                                            gcgcgcACCTGGGCGACGGACTGGCGGCGGCGTTGTGTAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGt                                                                         <  8:295054/149‑1 (MQ=255)
                                                                            gcgcgcACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGt                                                                         <  5:37878/149‑1 (MQ=255)
                                                                            gcgcgcACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGt                                                                         <  3:257340/149‑1 (MQ=255)
                                                                            gcgcgcACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGt                                                                         <  1:321642/149‑1 (MQ=255)
                                                                             cgcgcACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTg                                                                        <  3:237050/149‑1 (MQ=255)
                                                                                 cACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGcc                                                                    <  6:16693/149‑1 (MQ=255)
                                                                                  aCCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGccc                                                                   <  3:313134/149‑1 (MQ=255)
                                                                                         cGACGGACTGGCGGCGGCGTTGTGTAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGATACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGc                                                            <  4:183431/149‑1 (MQ=255)
                                                                                            cGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCtgt                                                         <  2:345488/149‑1 (MQ=255)
                                                                                                   gcggcggcgTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGa                                                   <  6:119513/148‑1 (MQ=255)
                                                                                                   gcggcggcgTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCATAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGAc                                                                <  7:77150/135‑1 (MQ=255)
                                                                                                   gcggcggcgTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCATAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGAc                                                                >  8:77150/1‑135 (MQ=255)
                                                                                                   gcggcggcgTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCATAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGAc                                                                >  8:72063/1‑135 (MQ=255)
                                                                                                   gcggcggcgTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCATAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGAc                                                                <  7:72063/135‑1 (MQ=255)
                                                                                                                 gAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAg                                                             <  7:62717/124‑1 (MQ=255)
                                                                                                                 gAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAg                                                             >  8:62717/1‑124 (MQ=255)
                                                                                                                                tGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGgtcg                     >  4:333766/1‑149 (MQ=255)
                                                                                                                                   gcgcgACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGgtcgtc                   >  4:18447/1‑148 (MQ=255)
                                                                                                                                       gACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATgg              <  7:330561/149‑1 (MQ=255)
                                                                                                                                          tACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATGgcgc           >  8:252868/1‑149 (MQ=255)
                                                                                                                                          tACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATGgcgc           >  8:71330/1‑149 (MQ=255)
                                                                                                                                              aGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATGGCGCGATc       >  1:243586/1‑149 (MQ=255)
                                                                                                                                               ggCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATGGCGCGATcc      >  5:81836/1‑149 (MQ=255)
                                                                                                                                                   ccTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATGGCGCGATCCGCCt  >  5:28260/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGCCCATGACGCCACTCACCCAGTTGCAAGTGTTCAACGCCATGCATGCCTCGCCCAATGCCAGGCTGGAGCTGAGCGCGCACCTGGGCGACGGACTGGCGGCGGCGTTGTGGAGCAACCGTGACGATGCGCGCGACTACCAGGCGCCTAGCCATCACACCTTGTCGTGCTACATCGCCGACGGCACCGGCACCTTCCGCCGCCAGCGCCCGGCCGACAAAGGTGCGCCCGACAAGCTGTGCGTGATGCCGGCCGGGCAGGAATCGAACTGGGTCGTCAATGGCGCGATCCGCCT  >  NC_002947/5225757‑5226052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑