Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,239,884 G→A 10.2% A144V (GCG→GTG)  ldh ← leucine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,239,8840GA10.2% 206.6 / 11.2 78A144V (GCG→GTG) ldhleucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (33/37);  new base A (3/5);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-02

CTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTC  >  NC_002947/5239739‑5240032
                                                                                                                                                 |                                                                                                                                                     
cTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCCGCCGGAACGAGGTTGTGGCCCGTATGCCCGCGAACACCCCCATCGCCGCAt                                                                                                                                                    >  5:375777/1‑149 (MQ=255)
    gcgcCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑gg                                                                                                                                               <  4:138113/149‑1 (MQ=255)
       cccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTTCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGa                                                                                                                                            <  7:340387/149‑1 (MQ=255)
        ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGAt                                                                                                                                           <  3:104687/149‑1 (MQ=255)
        ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGAt                                                                                                                                           >  6:7136/1‑149 (MQ=255)
          aCCGCATGCAATTTCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTTCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATgg                                                                                                                                         <  2:114936/149‑1 (MQ=255)
          aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACTGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATgg                                                                                                                                         <  2:231404/149‑1 (MQ=255)
          aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATgg                                                                                                                                         <  7:34365/149‑1 (MQ=255)
          aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgg                                                                                                                                         <  5:7136/149‑1 (MQ=255)
          aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgg                                                                                                                                         <  5:194394/149‑1 (MQ=255)
          aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgg                                                                                                                                         <  2:307481/149‑1 (MQ=255)
           ccGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATggg                                                                                                                                        >  5:96448/1‑149 (MQ=255)
            cGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGGGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgggg                                                                                                                                       <  5:372281/149‑2 (MQ=255)
             gCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTc                                                                                                                                      <  2:105226/149‑1 (MQ=255)
              cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgggggt                                                                                                                                     <  8:281850/149‑4 (MQ=255)
                tGCAATTGCTCCCCCAGGTCATATACCACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGTAACGAGGTTGGGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGcc                                                                                                                                   <  4:436/149‑1 (MQ=255)
                    aTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGAt                                                                                                                               >  7:95807/1‑149 (MQ=255)
                    aTTGCTCCCCCAGGGAATAACCAAAATTGCCCAACCCCTGGACCGCTCCCCCCAAGCCTCTCTGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCTAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGAt                                                                                                                               <  4:183121/149‑1 (MQ=255)
                         tCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgt                                                                                                                          >  8:264673/1‑149 (MQ=255)
                         tCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgt                                                                                                                          >  7:42888/1‑149 (MQ=255)
                         tCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgt                                                                                                                          >  6:153407/1‑149 (MQ=255)
                            gCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGATCACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgtggt                                                                                                                       <  6:147898/149‑1 (MQ=255)
                                gggCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTg                                                                                                                   <  4:99444/149‑1 (MQ=255)
                                    aTAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCa                                                                                                               >  6:339197/1‑149 (MQ=255)
                                       aCCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACg                                                                                                             >  1:313297/1‑148 (MQ=255)
                                        ccAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCatg                                                                                                             >  4:240834/1‑145 (MQ=255)
                                        ccAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGAAGTGGTGCTGGTCACGTa                                                                                                           >  8:348761/1‑149 (MQ=255)
                                         cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAg                                                                                                          >  7:38453/1‑149 (MQ=255)
                                            cATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                       <  8:90003/149‑1 (MQ=255)
                                                gCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                    <  7:5215/148‑1 (MQ=255)
                                                  ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtg                                                                                                 >  2:58597/1‑149 (MQ=255)
                                                    aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGACCGAGGTTGTGGCACGTATGCCCGCGACCACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATCCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                               >  7:68881/1‑149 (MQ=255)
                                                    aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                               <  8:38453/149‑1 (MQ=255)
                                                         cTGGACCGCGAACCGCAAGGCTCTCCGGTCATCGCTGCCCAGCCGGACCGTGGGTGTGGCACGTTTGCCGGCGAACCCCCCCTTCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCaa                                                                                          <  8:68881/149‑1 (MQ=255)
                                                            gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                        <  2:334913/148‑1 (MQ=255)
                                                             aCCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                      <  7:264673/149‑1 (MQ=255)
                                                               cGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGt                                                                                    >  7:351950/1‑149 (MQ=255)
                                                                    cccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                              >  1:71429/1‑134 (MQ=255)
                                                                    cccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                              <  2:71429/134‑1 (MQ=255)
                                                                    cccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATg                                                                               >  2:242240/1‑149 (MQ=255)
                                                                     ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGCGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGt                                                                              <  8:51377/149‑1 (MQ=255)
                                                                     ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTggg                                                                                             >  1:330445/1‑134 (MQ=255)
                                                                     ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTggg                                                                                             <  2:330445/134‑1 (MQ=255)
                                                                          aGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAg                                                                         <  5:339197/149‑1 (MQ=255)
                                                                              tctcAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGAGGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTc                                                                      >  2:247895/1‑148 (MQ=255)
                                                                                    gTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                               >  8:95071/1‑117 (MQ=255)
                                                                                    gTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                               <  7:95071/117‑1 (MQ=255)
                                                                                    gTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGc                                                               >  2:198868/1‑149 (MQ=255)
                                                                                     tCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                   >  7:331790/1‑144 (MQ=255)
                                                                                     tCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                   <  8:331790/144‑1 (MQ=255)
                                                                                       aTCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGTTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCAc                                                            <  1:287363/149‑1 (MQ=255)
                                                                                       aTCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCa                                                             <  1:242240/148‑1 (MQ=255)
                                                                                        tCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCACATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACt                                                           <  2:313297/149‑1 (MQ=255)
                                                                                           cTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTc                                                        <  8:95807/149‑1 (MQ=255)
                                                                                               ccAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACg                                                    >  6:191041/1‑149 (MQ=255)
                                                                                               ccAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACg                                                    >  2:275853/1‑149 (MQ=255)
                                                                                                cAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACg                                                    >  1:103919/1‑149 (MQ=255)
                                                                                                  gCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGc                                                  >  8:116438/1‑148 (MQ=255)
                                                                                                        aCGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatgat                                            >  5:282047/1‑148 (MQ=255)
                                                                                                                 tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTg                                                          >  1:106201/1‑125 (MQ=255)
                                                                                                                 tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTg                                                          <  2:106201/125‑1 (MQ=255)
                                                                                                                 tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGccc                                   <  5:153407/148‑1 (MQ=255)
                                                                                                                   gCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCAt                                 <  5:191041/148‑1 (MQ=255)
                                                                                                                   gCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCAt                                 <  8:42888/148‑1 (MQ=255)
                                                                                                                    cACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCAc                                                     >  7:318425/1‑127 (MQ=255)
                                                                                                                    cACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCAc                                                     <  8:318425/127‑1 (MQ=255)
                                                                                                                      cGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                             <  6:282047/149‑1 (MQ=255)
                                                                                                                      cGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                             <  1:275853/149‑1 (MQ=255)
                                                                                                                            cccGCGCACACCCCCATGGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTAt                        <  4:271493/148‑1 (MQ=255)
                                                                                                                             ccGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAAt                                                                                         <  2:106835/82‑1 (MQ=255)
                                                                                                                             ccGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCATGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATc                       >  5:144774/1‑148 (MQ=255)
                                                                                                                             ccGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCAAGTAGGGCGTGCTGTGGGCAAt                                                                                         >  1:106835/1‑82 (MQ=255)
                                                                                                                                 gAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGa                  <  1:198868/149‑1 (MQ=255)
                                                                                                                                       ccccATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGc            >  3:75425/1‑149 (MQ=255)
                                                                                                                                       ccccATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGc            >  3:95650/1‑149 (MQ=255)
                                                                                                                                        cccATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGaa                                         >  2:75930/1‑119 (MQ=255)
                                                                                                                                           aTCGCCACATGC‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa          >  6:133756/1‑147 (MQ=255)
                                                                                                                                           aTCGCCACATGC‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa          >  6:104760/1‑147 (MQ=255)
                                                                                                                                                 gcATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCTCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTc  <  1:247895/149‑1 (MQ=255)
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CTCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTC  >  NC_002947/5239739‑5240032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑