Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,239,889	T→C	10.2%	P142P (CCA→CCG)	ldh ←	leucine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,239,889	0	T	C	10.2%	201.1 / 12.1	78	P142P (CCA→CCG)	ldh	leucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (32/38);  new base C (3/5);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

GCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTCGAA  >  NC_002947/5239743‑5240035                                                                                                                                                   |                                                                                                                                                    gcgcCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑gg                                                                                                                                                  <  4:138113/149‑1 (MQ=255)    cccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTTCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGa                                                                                                                                               <  7:340387/149‑1 (MQ=255)     ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGAt                                                                                                                                              <  3:104687/149‑1 (MQ=255)     ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGAt                                                                                                                                              >  6:7136/1‑149 (MQ=255)       aCCGCATGCAATTTCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTTCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATgg                                                                                                                                            <  2:114936/149‑1 (MQ=255)       aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACTGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATgg                                                                                                                                            <  2:231404/149‑1 (MQ=255)       aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATgg                                                                                                                                            <  7:34365/149‑1 (MQ=255)       aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgg                                                                                                                                            <  5:7136/149‑1 (MQ=255)       aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgg                                                                                                                                            <  5:194394/149‑1 (MQ=255)       aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgg                                                                                                                                            <  2:307481/149‑1 (MQ=255)        ccGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATggg                                                                                                                                           >  5:96448/1‑149 (MQ=255)         cGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGGGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgggg                                                                                                                                          <  5:372281/149‑2 (MQ=255)          gCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTc                                                                                                                                         <  2:105226/149‑1 (MQ=255)           cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGC‑GGCGATgggggt                                                                                                                                        <  8:281850/149‑4 (MQ=255)             tGCAATTGCTCCCCCAGGTCATATACCACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGTAACGAGGTTGGGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGcc                                                                                                                                      <  4:436/149‑1 (MQ=255)                 aTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGAt                                                                                                                                  >  7:95807/1‑149 (MQ=255)                 aTTGCTCCCCCAGGGAATAACCAAAATTGCCCAACCCCTGGACCGCTCCCCCCAAGCCTCTCTGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCTAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGAt                                                                                                                                  <  4:183121/149‑1 (MQ=255)                      tCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgt                                                                                                                             >  8:264673/1‑149 (MQ=255)                      tCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgt                                                                                                                             >  6:153407/1‑149 (MQ=255)                      tCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgt                                                                                                                             >  7:42888/1‑149 (MQ=255)                         gCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGATCACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAgtggt                                                                                                                          <  6:147898/149‑1 (MQ=255)                             gggCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTg                                                                                                                      <  4:99444/149‑1 (MQ=255)                                 aTAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCa                                                                                                                  >  6:339197/1‑149 (MQ=255)                                    aCCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACg                                                                                                                >  1:313297/1‑148 (MQ=255)                                     ccAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCatg                                                                                                                >  4:240834/1‑145 (MQ=255)                                     ccAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGAAGTGGTGCTGGTCACGTa                                                                                                              >  8:348761/1‑149 (MQ=255)                                      cAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAg                                                                                                             >  7:38453/1‑149 (MQ=255)                                         cATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGc                                                                                                          <  8:90003/149‑1 (MQ=255)                                             gCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                       <  7:5215/148‑1 (MQ=255)                                               ccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtg                                                                                                    >  2:58597/1‑149 (MQ=255)                                                 aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGACCGAGGTTGTGGCACGTATGCCCGCGACCACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATCCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                                  >  7:68881/1‑149 (MQ=255)                                                 aaCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                                  <  8:38453/149‑1 (MQ=255)                                                      cTGGACCGCGAACCGCAAGGCTCTCCGGTCATCGCTGCCCAGCCGGACCGTGGGTGTGGCACGTTTGCCGGCGAACCCCCCCTTCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCaa                                                                                             <  8:68881/149‑1 (MQ=255)                                                         gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                           <  2:334913/148‑1 (MQ=255)                                                          aCCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                         <  7:264673/149‑1 (MQ=255)                                                            cGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGt                                                                                       >  7:351950/1‑149 (MQ=255)                                                                 cccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                                 <  2:71429/134‑1 (MQ=255)                                                                 cccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                                 >  1:71429/1‑134 (MQ=255)                                                                 cccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATg                                                                                  >  2:242240/1‑149 (MQ=255)                                                                  ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGCGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGt                                                                                 <  8:51377/149‑1 (MQ=255)                                                                  ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTggg                                                                                                >  1:330445/1‑134 (MQ=255)                                                                  ccGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTggg                                                                                                <  2:330445/134‑1 (MQ=255)                                                                       aGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAg                                                                            <  5:339197/149‑1 (MQ=255)                                                                           tctcAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGAGGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTc                                                                         >  2:247895/1‑148 (MQ=255)                                                                                 gTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                                  <  7:95071/117‑1 (MQ=255)                                                                                 gTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtgtg                                                                                                  >  8:95071/1‑117 (MQ=255)                                                                                 gTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGc                                                                  >  2:198868/1‑149 (MQ=255)                                                                                  tCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                      <  8:331790/144‑1 (MQ=255)                                                                                  tCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                      >  7:331790/1‑144 (MQ=255)                                                                                    aTCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGTTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCAc                                                               <  1:287363/149‑1 (MQ=255)                                                                                    aTCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCa                                                                <  1:242240/148‑1 (MQ=255)                                                                                     tCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCACATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACt                                                              <  2:313297/149‑1 (MQ=255)                                                                                        cTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTc                                                           <  8:95807/149‑1 (MQ=255)                                                                                            ccAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACg                                                       >  6:191041/1‑149 (MQ=255)                                                                                            ccAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACg                                                       >  2:275853/1‑149 (MQ=255)                                                                                             cAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACg                                                       >  1:103919/1‑149 (MQ=255)                                                                                               gCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGc                                                     >  8:116438/1‑148 (MQ=255)                                                                                                     aCGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatgat                                               >  5:282047/1‑148 (MQ=255)                                                                                                              tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTg                                                             >  1:106201/1‑125 (MQ=255)                                                                                                              tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTg                                                             <  2:106201/125‑1 (MQ=255)                                                                                                              tgGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGccc                                      <  5:153407/148‑1 (MQ=255)                                                                                                                gCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCAt                                    <  8:42888/148‑1 (MQ=255)                                                                                                                gCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCAt                                    <  5:191041/148‑1 (MQ=255)                                                                                                                 cACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCAc                                                        >  7:318425/1‑127 (MQ=255)                                                                                                                 cACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCAc                                                        <  8:318425/127‑1 (MQ=255)                                                                                                                   cGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                                <  6:282047/149‑1 (MQ=255)                                                                                                                   cGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCaa                                <  1:275853/149‑1 (MQ=255)                                                                                                                         cccGCGCACACCCCCATGGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTAt                           <  4:271493/148‑1 (MQ=255)                                                                                                                          ccGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAAt                                                                                            <  2:106835/82‑1 (MQ=255)                                                                                                                          ccGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCATGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATc                          >  5:144774/1‑148 (MQ=255)                                                                                                                          ccGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCAAGTAGGGCGTGCTGTGGGCAAt                                                                                            >  1:106835/1‑82 (MQ=255)                                                                                                                              gAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGa                     <  1:198868/149‑1 (MQ=255)                                                                                                                                    ccccATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGc               >  3:95650/1‑149 (MQ=255)                                                                                                                                    ccccATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGc               >  3:75425/1‑149 (MQ=255)                                                                                                                                     cccATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGaa                                            >  2:75930/1‑119 (MQ=255)                                                                                                                                        aTCGCCACATGC‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa             >  6:104760/1‑147 (MQ=255)                                                                                                                                        aTCGCCACATGC‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa             >  6:133756/1‑147 (MQ=255)                                                                                                                                              gcATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCTCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTc     <  1:247895/149‑1 (MQ=255)                                                                                                                                                   t‑gGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTCGaa  >  8:321552/1‑147 (MQ=255)                                                                                                                                                   t‑gGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTCGaa  >  5:268716/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGT‑GGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTCGAA  >  NC_002947/5239743‑5240035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑