Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,253,887 G→C 7.1% F249L (TTC→TTG)  PP_4630 ← MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,253,8870GC 7.1% 166.1 / 4.2 56F249L (TTC→TTG) PP_4630MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/29);  new base C (2/2);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GGGCCAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGC  >  NC_002947/5253739‑5254026
                                                                                                                                                    |                                                                                                                                            
gggCCAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTg                                                                                                                                              <  3:323438/149‑1 (MQ=255)
   ccAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAg                                                                                                                                           >  5:110404/1‑149 (MQ=255)
    cAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGt                                                                                                                                          <  4:36480/149‑1 (MQ=255)
     aGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACACCAGGCGCTTGAAGTg                                                                                                                                         >  7:47614/1‑149 (MQ=255)
        aaCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCt                                                                                                                                      <  1:12282/149‑1 (MQ=255)
         aCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTg                                                                                                                                     <  4:211650/149‑1 (MQ=255)
         aCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCACAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTg                                                                                                                                     <  2:125994/149‑1 (MQ=255)
              cGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAg                                                                                                                                >  3:202924/1‑149 (MQ=255)
               gCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGt                                                                                                                               >  8:266611/1‑149 (MQ=255)
                   gcgcTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCTCCAGCCAGTAACAAGGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTctg                                                                                                                           <  4:202924/149‑1 (MQ=255)
                     gcTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTctgct                                                                                                                         <  3:161305/149‑1 (MQ=255)
                        ggCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                      <  1:245864/149‑1 (MQ=255)
                         gcgcATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTgg                                                                                                                     >  1:248671/1‑149 (MQ=255)
                           gcATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGACTGCCAGCCCGGCTGGCCTGTACCGCTTCCGCTGCGTGACCAGCCAGTTACAACGGGCATTGCAGCATCGACGCCCGGCGCGTGAAGTGCCTGGGGAGTTCTGCTGTGGGc                                                                                                                   >  1:257633/1‑149 (MQ=255)
                                  acTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                            >  3:316191/1‑149 (MQ=255)
                                   cTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATg                                                                                                           <  7:301687/149‑1 (MQ=255)
                                    tGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATgg                                                                                                          >  7:169807/1‑149 (MQ=255)
                                        cTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGaa                                                                                                       >  3:241695/1‑148 (MQ=255)
                                           cgcgcCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACACCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGaaaa                                                                                                     <  8:47614/147‑1 (MQ=255)
                                              gcCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCa                                                                                                <  6:110404/149‑1 (MQ=255)
                                                    gcaggcTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAggg                                                                                          >  4:185402/1‑149 (MQ=255)
                                                        gcTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTc                                                                                      >  7:203266/1‑149 (MQ=255)
                                                          tACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCg                                                                                    >  3:142506/1‑149 (MQ=255)
                                                             aGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCAGCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACGGATGGGAAAACAGCACCAGGGCGTCCGGCt                                                                                 >  8:76692/1‑149 (MQ=255)
                                                               ggCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTc                                                                               <  1:368336/149‑1 (MQ=255)
                                                                gCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGTGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCa                                                                              <  1:258390/149‑1 (MQ=255)
                                                                  tGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGTCTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGc                                                                            >  1:115846/1‑149 (MQ=255)
                                                                  tGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGc                                                                            >  1:139382/1‑149 (MQ=255)
                                                                      agccagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGtt                                                                        <  4:142506/149‑1 (MQ=255)
                                                                      agccagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGt                                                                         >  8:104781/1‑148 (MQ=255)
                                                                         cagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCg                                                                      >  2:213223/1‑148 (MQ=255)
                                                                            gCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGa                                                                  <  8:165337/149‑1 (MQ=255)
                                                                                  cTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAg‑c                                                            <  8:180253/148‑1 (MQ=255)
                                                                                        cGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCt                                                     >  1:51168/1‑149 (MQ=255)
                                                                                         gCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTc                                                    <  7:76692/149‑1 (MQ=255)
                                                                                          cTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTcc                                                   <  3:185402/149‑1 (MQ=255)
                                                                                          cTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTcc                                                   <  4:316191/149‑1 (MQ=255)
                                                                                           ttCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATg                                                                                                           <  2:1680/93‑1 (MQ=255)
                                                                                             ccGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTg                                                 <  1:213223/148‑1 (MQ=255)
                                                                                                ctgctTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGc                                             <  2:51168/149‑1 (MQ=255)
                                                                                                    ttCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCTGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCt                                         >  2:257560/1‑149 (MQ=255)
                                                                                                        ccagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGccc                                     <  2:178706/149‑1 (MQ=255)
                                                                                                         cagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGGTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCg                                    >  7:168856/1‑149 (MQ=255)
                                                                                                         cagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCg                                    >  3:304446/1‑149 (MQ=255)
                                                                                                            ccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTc                                 <  7:323046/149‑1 (MQ=255)
                                                                                                                   caacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCg                          <  7:266611/149‑1 (MQ=255)
                                                                                                                      cGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCTGGCTTCAGCCGTTCGCAGACCAGCCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCggg                        <  1:257560/149‑1 (MQ=255)
                                                                                                                       gggCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGAt                      >  5:382823/1‑149 (MQ=255)
                                                                                                                        ggCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATc                     <  7:104781/149‑1 (MQ=255)
                                                                                                                            ttCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGTCTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCAcgc                 <  2:115846/149‑1 (MQ=255)
                                                                                                                               cAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCgt               <  4:241695/148‑1 (MQ=255)
                                                                                                                                  cATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGttt           >  1:74313/1‑149 (MQ=255)
                                                                                                                                      gACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTtcgtc       <  8:203266/149‑1 (MQ=255)
                                                                                                                                       tcGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAg                                                       <  2:279576/99‑1 (MQ=39)
                                                                                                                                       tcGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAg                                                       >  1:279576/2‑100 (MQ=39)
                                                                                                                                       tcGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTtcgtcg      >  6:78166/2‑149 (MQ=255)
                                                                                                                                          ccAGGCACTTCAAGCGCCTGGCG‑TCGATGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGc  <  2:74336/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GGGCCAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGC  >  NC_002947/5253739‑5254026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑