Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,253,891 T→C 7.1% H248R (CAC→CGC)  PP_4630 ← MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,253,8910TC 7.1% 158.2 / 2.7 56H248R (CAC→CGC) PP_4630MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (23/29);  new base C (2/2);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

CAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCT  >  NC_002947/5253743‑5254039
                                                                                                                                                    |                                                                                                                                                     
cAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGt                                                                                                                                                       <  4:36480/149‑1 (MQ=255)
 aGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACACCAGGCGCTTGAAGTg                                                                                                                                                      >  7:47614/1‑149 (MQ=255)
    aaCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCt                                                                                                                                                   <  1:12282/149‑1 (MQ=255)
     aCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTg                                                                                                                                                  <  4:211650/149‑1 (MQ=255)
     aCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCACAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTg                                                                                                                                                  <  2:125994/149‑1 (MQ=255)
          cGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAg                                                                                                                                             >  3:202924/1‑149 (MQ=255)
           gCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGt                                                                                                                                            >  8:266611/1‑149 (MQ=255)
               gcgcTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCTCCAGCCAGTAACAAGGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTctg                                                                                                                                        <  4:202924/149‑1 (MQ=255)
                 gcTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTctgct                                                                                                                                      <  3:161305/149‑1 (MQ=255)
                    ggCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                                   <  1:245864/149‑1 (MQ=255)
                     gcgcATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTgg                                                                                                                                  >  1:248671/1‑149 (MQ=255)
                       gcATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGACTGCCAGCCCGGCTGGCCTGTACCGCTTCCGCTGCGTGACCAGCCAGTTACAACGGGCATTGCAGCATCGACGCCCGGCGCGTGAAGTGCCTGGGGAGTTCTGCTGTGGGc                                                                                                                                >  1:257633/1‑149 (MQ=255)
                              acTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                                         >  3:316191/1‑149 (MQ=255)
                               cTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATg                                                                                                                        <  7:301687/149‑1 (MQ=255)
                                tGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATgg                                                                                                                       >  7:169807/1‑149 (MQ=255)
                                    cTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGaa                                                                                                                    >  3:241695/1‑148 (MQ=255)
                                       cgcgcCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACACCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGaaaa                                                                                                                  <  8:47614/147‑1 (MQ=255)
                                          gcCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCa                                                                                                             <  6:110404/149‑1 (MQ=255)
                                                gcaggcTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAggg                                                                                                       >  4:185402/1‑149 (MQ=255)
                                                    gcTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTc                                                                                                   >  7:203266/1‑149 (MQ=255)
                                                      tACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCg                                                                                                 >  3:142506/1‑149 (MQ=255)
                                                         aGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCAGCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACGGATGGGAAAACAGCACCAGGGCGTCCGGCt                                                                                              >  8:76692/1‑149 (MQ=255)
                                                           ggCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTc                                                                                            <  1:368336/149‑1 (MQ=255)
                                                            gCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGTGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCa                                                                                           <  1:258390/149‑1 (MQ=255)
                                                              tGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGTCTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGc                                                                                         >  1:115846/1‑149 (MQ=255)
                                                              tGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGc                                                                                         >  1:139382/1‑149 (MQ=255)
                                                                  agccagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGtt                                                                                     <  4:142506/149‑1 (MQ=255)
                                                                  agccagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGt                                                                                      >  8:104781/1‑148 (MQ=255)
                                                                     cagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCg                                                                                   >  2:213223/1‑148 (MQ=255)
                                                                        gCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGa                                                                               <  8:165337/149‑1 (MQ=255)
                                                                              cTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAg‑c                                                                         <  8:180253/148‑1 (MQ=255)
                                                                                    cGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCt                                                                  >  1:51168/1‑149 (MQ=255)
                                                                                     gCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTc                                                                 <  7:76692/149‑1 (MQ=255)
                                                                                      cTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTcc                                                                <  3:185402/149‑1 (MQ=255)
                                                                                      cTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTcc                                                                <  4:316191/149‑1 (MQ=255)
                                                                                       ttCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATg                                                                                                                        <  2:1680/93‑1 (MQ=255)
                                                                                         ccGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTg                                                              <  1:213223/148‑1 (MQ=255)
                                                                                            ctgctTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGc                                                          <  2:51168/149‑1 (MQ=255)
                                                                                                ttCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCTGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCt                                                      >  2:257560/1‑149 (MQ=255)
                                                                                                    ccagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGccc                                                  <  2:178706/149‑1 (MQ=255)
                                                                                                     cagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGGTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCg                                                 >  7:168856/1‑149 (MQ=255)
                                                                                                     cagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCg                                                 >  3:304446/1‑149 (MQ=255)
                                                                                                        ccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTc                                              <  7:323046/149‑1 (MQ=255)
                                                                                                               caacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCg                                       <  7:266611/149‑1 (MQ=255)
                                                                                                                  cGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCTGGCTTCAGCCGTTCGCAGACCAGCCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCggg                                     <  1:257560/149‑1 (MQ=255)
                                                                                                                   gggCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGAt                                   >  5:382823/1‑149 (MQ=255)
                                                                                                                    ggCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATc                                  <  7:104781/149‑1 (MQ=255)
                                                                                                                        ttCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGTCTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCAcgc                              <  2:115846/149‑1 (MQ=255)
                                                                                                                           cAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCgt                            <  4:241695/148‑1 (MQ=255)
                                                                                                                              cATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGttt                        >  1:74313/1‑149 (MQ=255)
                                                                                                                                  gACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTtcgtc                    <  8:203266/149‑1 (MQ=255)
                                                                                                                                   tcGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAg                                                                    >  1:279576/2‑100 (MQ=39)
                                                                                                                                   tcGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAg                                                                    <  2:279576/99‑1 (MQ=39)
                                                                                                                                   tcGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTtcgtcg                   >  6:78166/2‑149 (MQ=255)
                                                                                                                                      ccAGGCACTTCAAGCGCCTGGCG‑TCGATGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGc               <  2:74336/149‑1 (MQ=255)
                                                                                                                                                   gTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCTTCGCTTCCCAGGATCAGGc   >  6:283354/1‑149 (MQ=255)
                                                                                                                                                   gTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGc   <  2:74313/149‑1 (MQ=255)
                                                                                                                                                    tGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCt  >  4:226093/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAG‑CGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCT  >  NC_002947/5253743‑5254039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑