| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,262,496 | T→A | 6.2% | T223T (ACT→ACA) | PP_4638 → | methylenetetrahydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,262,496 | 0 | T | A | 6.2% | 276.5 / 3.5 | 89 | T223T (ACT→ACA) | PP_4638 | methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (38/45); new base A (4/2); total (42/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCA > NC_002947/5262348‑5262642 | tCACCTTGCAGCTGGGCTTCGATGCCTCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCCTGAGCCTCACCCACGGGCGCGCGACGAGGCTCGCCCACGTGGCCGGCCTGACt > 6:336501/1‑149 (MQ=255)tCACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACt > 5:320519/1‑149 (MQ=255) cACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTg > 1:271498/1‑149 (MQ=255) cACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTg > 7:63361/1‑149 (MQ=255) cTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCa > 1:56684/1‑149 (MQ=255) cAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACAGTCACGcc < 4:159698/149‑1 (MQ=255) cTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGccatc > 3:187713/1‑149 (MQ=255) ggCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGccatccat > 3:6351/1‑149 (MQ=255) cGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATgcgc < 5:336501/148‑1 (MQ=255) aTGCCGCCAAGCATCAGGAAGCCATACACTGGTTGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGAc < 7:319745/149‑1 (MQ=255) aTGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGAc < 1:80288/149‑1 (MQ=255) ccgccAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATg < 4:70473/149‑1 (MQ=255) gccAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCt < 2:56684/149‑1 (MQ=255) gccAACCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGATGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCCCGCCATCCATGCGCGACATGCt > 6:135398/1‑149 (MQ=255) cAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTc > 2:178581/1‑148 (MQ=255) aGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCg > 3:221836/1‑147 (MQ=255) aGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCg > 8:273820/1‑146 (MQ=255) aGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGga < 5:107896/148‑1 (MQ=255) aGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATCCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCTAATCGgag < 8:242346/149‑1 (MQ=255) ggAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGgaga < 2:371982/149‑1 (MQ=255) aTACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGgcg > 4:19416/1‑147 (MQ=255) acacTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGt > 2:321172/1‑149 (MQ=255) acTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTc > 6:265151/1‑148 (MQ=255) acTGGATGCGCCGTCAGGCCTCACCCAAGACCATGCTGGCCATCCTCATGAGCCTCACCCACGGGCGCGCGACGAAGCTCGACCACGTGGCCGGCCTGACAGTCACGCCATCCATGCGCGACATGCTCTAAGCGGACACGGCGCAGTc > 8:79572/1‑148 (MQ=255) cTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTcc > 3:57878/1‑148 (MQ=255) tGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGCTGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTcc > 7:297029/1‑147 (MQ=255) gATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCGACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCaa < 2:186551/147‑1 (MQ=255) gATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAgg > 1:222840/1‑149 (MQ=255) gATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAgg < 4:28746/149‑1 (MQ=255) gATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAg < 4:57878/148‑1 (MQ=255) gATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAg < 2:271498/148‑1 (MQ=255) gcCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCttt < 4:80034/149‑1 (MQ=255) ggCCTCACCCAAGCCCATGCTGGCCTGCCTCATTACCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAgg < 4:215865/149‑1 (MQ=255) gCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAgg > 5:56135/1‑148 (MQ=255) gCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAgg < 6:56135/148‑1 (MQ=255) gCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc < 4:181749/149‑1 (MQ=255) gCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc < 1:178581/149‑1 (MQ=255) cTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAg > 3:35173/1‑145 (MQ=255) cTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAg < 4:35173/145‑1 (MQ=255) tCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgcg > 5:259168/1‑149 (MQ=255) ccAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAgt > 5:228759/1‑149 (MQ=255) caGCCCATGCTGGCGTGCCTACTGAGCCTCTCCCACGTGGGCGCGACGATGCTCGACCACGTGGCCGTCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGAAgtgt < 8:63361/148‑1 (MQ=255) aGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt < 4:221836/149‑1 (MQ=255) aGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt < 1:321172/149‑1 (MQ=255) aGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt < 7:273820/149‑1 (MQ=255) gCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt > 4:95327/1‑148 (MQ=255) gCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTg < 6:259168/149‑1 (MQ=255) ccATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGAc > 3:244766/1‑149 (MQ=255) ggCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTgg < 8:297029/149‑1 (MQ=255) tgcctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATGCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGATGACCGCCTGGCCtt > 8:304756/1‑149 (MQ=255) cctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGTCCAGGCGCGCAGTGTTGACCTCCTGGCCtt < 4:150322/147‑1 (MQ=255) cctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTg > 5:283573/1‑148 (MQ=255) cctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTg < 5:139964/148‑1 (MQ=255) cctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGc < 3:19416/149‑1 (MQ=255) ctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGACGCGGAGCCGGCGCAGTCCAAGGCCTTTGCCCGGGCGCGCAGGGTGGAGCGCCTGGCCTTGcc > 8:248940/1‑148 (MQ=255) ctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCa < 5:135398/149‑1 (MQ=255) ctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCCCAGTGTTGACCGCCTGGCCTTGCa < 7:54916/149‑1 (MQ=255) aTGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATGCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGa < 7:304756/148‑1 (MQ=255) gAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTa > 2:355910/1‑149 (MQ=255) aGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTa < 5:265151/148‑1 (MQ=255) aGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTa < 1:38375/148‑1 (MQ=255) gCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTAt < 4:6351/148‑1 (MQ=255) tCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATc > 3:155994/1‑146 (MQ=255) tCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATc < 4:155994/146‑1 (MQ=255) cACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGt > 2:205785/1‑148 (MQ=255) aCCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGAt < 5:368975/140‑1 (MQ=255) aCCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACAAGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGAt > 6:368975/1‑140 (MQ=255) ccACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACa > 2:130655/1‑148 (MQ=255) aCGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAg < 3:95327/149‑1 (MQ=255) gggCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCt > 4:339240/1‑149 (MQ=255) gcgACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATgt > 1:333397/1‑147 (MQ=255) gacgaTGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGc > 5:114623/1‑148 (MQ=255) gacgaTGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGc > 8:167296/1‑148 (MQ=255) cgaTGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTAc < 6:320519/149‑1 (MQ=255) cTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCg < 6:283573/148‑1 (MQ=255) tCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgt > 1:344322/1‑149 (MQ=255) tCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGAGCGCAGAGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATCGGCTACGCCGgt > 4:232038/1‑149 (MQ=255) gcgcccGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgtgt < 1:235474/144‑1 (MQ=255) gACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgt < 3:339240/147‑1 (MQ=255) aCCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgtgt > 1:185284/1‑148 (MQ=255) aCCACGTGGCCGGCGTGAATGTCACGACATCCATGCGCGACATGATCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGtgtg < 2:333397/149‑1 (MQ=255) cACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCa < 4:323804/149‑1 (MQ=255) cACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCa < 2:255833/149‑1 (MQ=255) atggCGTGACAGTCACGCCGTCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGt > 2:100367/3‑147 (MQ=255) atggCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGt > 5:243128/3‑147 (MQ=255) atggCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATCGGCTACGCCGGTGTGCACCTGt > 2:35522/3‑147 (MQ=255) cGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAg > 4:59236/1‑149 (MQ=255) ggCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAgg > 8:241271/1‑149 (MQ=255) gCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAggg < 4:187713/149‑1 (MQ=255) tGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATCGGCTACGCCGGTGTGCACCTGTCAGGGGtt < 1:35522/149‑1 (MQ=255) cTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTca < 7:167296/148‑1 (MQ=255) | TCACCTTGCAGCTGGGCTTCGATGCCGCCAAGCATCAGGAAGCCATACACTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCA > NC_002947/5262348‑5262642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |