Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 465,447 T→A 6.1% D122V (GAT→GTT) ‡ PP_0384 ← AsnC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947465,4470TA 6.1% 196.3 / 2.6 65D122V (GAT→GTT) ‡PP_0384AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base A (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

CTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACG  >  NC_002947/465303‑465594
                                                                                                                                                |                                                                                                                                                   
cTCCTACACCCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAg                                                                                                                                                 <  8:288714/149‑1 (MQ=255)
   cTACAACCGGCCTCATCGATAACCCCACCCGCGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAgctg                                                                                                                                              >  5:96762/1‑149 (MQ=255)
   cTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAgct                                                                                                                                               <  5:141079/148‑1 (MQ=255)
    tACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAgctgc                                                                                                                                             >  6:23525/1‑149 (MQ=255)
        aCCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGATGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCga                                                                                                                                         <  2:267108/149‑2 (MQ=255)
        aCCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGATGTCAAGGTTCCACCTTGCTGCTGATAATGATCGACGTGGTGGTTTTCTCCACACCGTAGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCga                                                                                                                                         <  6:343918/149‑2 (MQ=255)
          cGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGc                                                                                                                                       <  3:76159/149‑1 (MQ=255)
          cGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGc                                                                                                                                       <  8:265908/149‑1 (MQ=255)
            gCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGa                                                                                                                                     <  7:217634/149‑1 (MQ=255)
             ccTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAg                                                                                                                                    <  7:67583/149‑1 (MQ=255)
                 aTCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCg                                                                                                                                <  8:314166/149‑1 (MQ=255)
                 aTCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGACCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTc                                                                                                                                  <  2:127836/147‑1 (MQ=255)
                   cGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTa                                                                                                                              >  5:272429/1‑149 (MQ=255)
                    gATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTAc                                                                                                                             >  8:153743/1‑149 (MQ=255)
                                   gcgcCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACAt                                                                                                              <  6:349527/149‑1 (MQ=255)
                                   gcgcCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACAt                                                                                                              <  6:353598/149‑1 (MQ=255)
                                       cAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGc                                                                                                                   <  6:233580/140‑1 (MQ=255)
                                       cAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGc                                                                                                                   >  5:233580/1‑140 (MQ=255)
                                           ccGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAAt                                                                                                      >  8:275934/1‑149 (MQ=255)
                                              ttCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATcc                                                                                                                                                   >  5:359295/1‑101 (MQ=255)
                                              ttCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATcc                                                                                                                                                   <  6:359295/101‑1 (MQ=255)
                                               tCAAAGCGGCTGTCCACGGTCCTCCTTGCTGCAGCGAATGATCGACGTGTTGGTTTTCTCCACACCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTcgc                                                                                                  <  8:262206/149‑1 (MQ=255)
                                                    gCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCt                                                                                              >  7:333221/1‑148 (MQ=255)
                                                             cACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGCCACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                                     >  4:331909/1‑148 (MQ=255)
                                                              aCGGTCCACCTTGCTGATGAGAATGATCGACGTGGTGGTTTTCTCCACCCAGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGCGCGAGTACGTACGCAGCCAGCCGACATAGTCGAATTAGCCGCTTACCGcacaca                                                                                   >  6:53192/1‑149 (MQ=255)
                                                                 gTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAgct                                                                                <  2:46625/149‑1 (MQ=255)
                                                                  tCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAgctg                                                                               <  7:153743/149‑1 (MQ=255)
                                                                   ccACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAgctg                                                                               <  8:333221/148‑1 (MQ=255)
                                                                    cACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAgctgct                                                                             >  7:155727/1‑149 (MQ=255)
                                                                         tgctgcTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCAcc                                                                        >  7:343840/1‑149 (MQ=255)
                                                                           ctgctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTg                                                                      <  1:32939/149‑1 (MQ=255)
                                                                            tgctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTgg                                                                     <  8:239967/149‑1 (MQ=255)
                                                                            tgctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTgg                                                                     >  4:37543/1‑149 (MQ=255)
                                                                                 agaATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCAt                                                                <  8:155727/149‑1 (MQ=255)
                                                                                  gaATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATg                                                               <  8:22246/149‑1 (MQ=255)
                                                                                   aaTGATCGTCGTGGTGTTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATgg                                                              <  6:380107/149‑1 (MQ=255)
                                                                                   aaTGATCGACGTGGTGGTTTTCTCCCCCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATgg                                                              >  3:14478/1‑149 (MQ=255)
                                                                                   aaTGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATgg                                                              >  7:168602/1‑149 (MQ=255)
                                                                                     ttaTCGACGTGGTGGTTTTCTCCACCCCGTGGATACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCTAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGc                                                             <  5:53192/146‑1 (MQ=255)
                                                                                     tGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCa                                                            <  6:96762/149‑1 (MQ=255)
                                                                                      gATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCAc                                                           >  4:86636/1‑149 (MQ=255)
                                                                                       aTCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACt                                                          <  5:23525/149‑1 (MQ=255)
                                                                                                  ggtTTTCTCCACCCCGTCGACACTGCCAATGTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGTAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGca                                               >  1:31791/1‑149 (MQ=255)
                                                                                                  ggtTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGcgccgcc                                               >  8:177627/1‑148 (MQ=255)
                                                                                                  ggtTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGca                                               >  2:342337/1‑149 (MQ=255)
                                                                                                  ggtTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGca                                               >  2:163664/1‑149 (MQ=255)
                                                                                                     tttCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCCCACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcacca                                            >  2:75153/1‑149 (MQ=255)
                                                                                                     tttCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcacca                                            >  3:217135/1‑149 (MQ=255)
                                                                                                     gaaaaccACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCAcc                                                                        >  8:239363/6‑121 (MQ=255)
                                                                                                     gaaaaccACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCAcc                                                                        <  7:239363/116‑1 (MQ=255)
                                                                                                        ctcCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACg                                          <  6:272429/148‑1 (MQ=255)
                                                                                                         tcCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTc                                        >  2:278920/1‑149 (MQ=255)
                                                                                                                        cTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTgg                         <  1:195375/149‑1 (MQ=255)
                                                                                                                        cTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTgg                         <  4:14478/149‑1 (MQ=255)
                                                                                                                        cTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTgg                         <  4:217135/149‑1 (MQ=255)
                                                                                                                         tGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGc                        >  2:100240/1‑149 (MQ=255)
                                                                                                                           ccAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTg                      >  6:57398/1‑149 (MQ=255)
                                                                                                                                   gTCGATCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCt                 <  8:343840/146‑1 (MQ=255)
                                                                                                                                    ccGAGCAGCTGGATCAGTTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCGATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGAt             >  1:308960/2‑149 (MQ=255)
                                                                                                                                     cGAGCAGCTGGATCAGTTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATc            >  3:53655/1‑149 (MQ=255)
                                                                                                                                        gCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCg         <  1:100240/149‑1 (MQ=255)
                                                                                                                                          aaCTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCtt                                     <  1:110916/119‑1 (MQ=255)
                                                                                                                                          aaCTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCtt                                     >  2:110916/1‑119 (MQ=255)
                                                                                                                                          aaCTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGa        >  8:49277/1‑148 (MQ=255)
                                                                                                                                               aTCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACg  >  2:235825/1‑149 (MQ=255)
                                                                                                                                                tCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGc                                   <  7:331608/115‑1 (MQ=255)
                                                                                                                                                tCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGc                                   >  8:331608/1‑115 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACG  >  NC_002947/465303‑465594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑