Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	468,464	A→G	6.6%	C831C (TGT→TGC)	PP_0386 ←	sensory box protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	468,464	0	A	G	6.6%	265.1 / 6.3	94	C831C (TGT→TGC)	PP_0386	sensory box protein
Reads supporting (aligned to +/- strand):  ref base A (37/49);  new base G (3/3);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

TCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTGGGTGAT  >  NC_002947/468318‑468610                                                                                                                                                    |                                                                                                                                                    tCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGca                                                                                                                                                     >  5:274690/1‑147 (MQ=255)   aaCGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAgg                                                                                                                                                 >  6:306246/1‑149 (MQ=255)    aCGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGt                                                                                                                                                <  4:141509/149‑1 (MQ=255)    aCGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGt                                                                                                                                                <  8:262293/149‑1 (MQ=255)      gTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGGCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCTCTGTGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                              <  6:124164/149‑1 (MQ=255)      gTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                              <  5:123467/149‑1 (MQ=255)      gTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                              <  6:309139/149‑1 (MQ=255)        cgcgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGt                                                                                                                                            <  7:27910/149‑1 (MQ=255)         gcgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGCAAGGACCCCGCCCGGGTGGCCAAGGCTGACGTTGATGTGCTTGAGGCGGTCGAGGTCCAGGAACAGCACTCCGCCCTGGCGGTTGGACACCTGCGCACAGGTCAg                                                                                                                                             >  1:73299/1‑148 (MQ=255)         gcgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGTGCGCAGGTgtcc                                                                                                                                            <  4:322505/148‑4 (MQ=255)         gcgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGt                                                                                                                                            <  1:339722/148‑1 (MQ=255)         gcgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGt                                                                                                                                            <  5:76857/148‑1 (MQ=255)         gcgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTa                                                                                                                                           >  1:71054/1‑149 (MQ=255)          cgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                              <  6:61840/145‑1 (MQ=255)          cgTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                              >  5:61840/1‑145 (MQ=255)              ccTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                              >  5:29440/1‑141 (MQ=255)              ccTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGa                                                                                                                                              <  6:29440/141‑1 (MQ=255)              ccTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGccc                                                                                                                                      <  7:278519/149‑1 (MQ=255)               cTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCt                                                                                                                                     >  5:183399/1‑149 (MQ=255)                 gCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTg                                                                                                                                    >  5:85009/1‑148 (MQ=255)                 actctTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCCCAGGTGAGTACGCCCTg                                                                                                                                    >  5:224544/2‑148 (MQ=255)                 actctTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTg                                                                                                                                    <  6:224544/147‑1 (MQ=255)                   tctTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAg                                                                                                                                 >  8:35962/1‑149 (MQ=255)                     tttCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGcc                                                                                                                               >  6:230838/1‑149 (MQ=255)                       tCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGc                                                                                                                                <  2:97017/146‑1 (MQ=255)                            cgcTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTc                                                                                                                        <  6:183399/149‑1 (MQ=255)                            cgcTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTc                                                                                                                        <  5:230838/149‑1 (MQ=255)                                    gATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAgg                                                                                                                <  5:306246/149‑1 (MQ=255)                                         ccTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACaa                                                                                                                  >  2:35470/1‑142 (MQ=255)                                         ccTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACaa                                                                                                                  <  1:35470/142‑1 (MQ=255)                                         ccTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGa                                                                                                           >  6:109425/1‑149 (MQ=255)                                         ccTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGAGGTTGATGTCCTTGAAGCGGTCGAGGTCCAGGAACAGCACTTCGCCCTGGCGGTTGGACAACTGCGAAAACGTGACTACGAGCTGGAGCCGGTGTTCGACCAATGTGCGa                                                                                                           >  8:155306/1‑149 (MQ=255)                                          cTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGAt                                                                                                          >  8:339904/1‑149 (MQ=255)                                           ttGAGCAGC‑AGGTCGCAGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGAtt                                                                                                         >  2:259404/1‑149 (MQ=255)                                              agcagc‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTgg                                                                                                       <  4:201441/148‑1 (MQ=255)                                                cagc‑agGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTggg                                                                                                      >  1:217041/1‑147 (MQ=255)                                                cagc‑agGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTggg                                                                                                      >  5:129245/1‑147 (MQ=255)                                                cagc‑agGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCa                                                                                                    <  5:109425/149‑1 (MQ=255)                                                   c‑agGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGc                                                                                                 <  1:362673/149‑1 (MQ=255)                                                            cGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCTCTGGTGTGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGc                                                                                        >  1:312677/1‑149 (MQ=255)                                                             gACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCg                                                                                       >  5:90979/1‑149 (MQ=255)                                                                 ggggggCCACGGCTGTCGTTGCTGTGCTTGTCGCGGTCGAGGTCCCGGAACAGCTCTGCGCCCTGGCGGTTGGACTCCTTCGCACAGGGGAGTACGCCCGGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTc                                                                                   <  8:265794/149‑1 (MQ=255)                                                                   gTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCgt                                                                                 >  4:245877/1‑149 (MQ=255)                                                                    tGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCgtg                                                                                <  3:274041/149‑1 (MQ=255)                                                                       ccAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGcc                                                                                                                               <  6:265047/100‑1 (MQ=255)                                                                       ccAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGcc                                                                                                                               >  5:265047/1‑100 (MQ=255)                                                                        cAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGGCGtggtgg                                                                            <  6:274690/149‑1 (MQ=255)                                                                          aGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGc                                                                          <  3:348806/149‑1 (MQ=255)                                                                          aGGCTGTCGTTGATGTGCTGGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGc                                                                          <  2:73299/149‑1 (MQ=255)                                                                            gCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCtt                                                                        <  2:217041/149‑1 (MQ=255)                                                                              tGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGa                                                                      <  3:116247/149‑1 (MQ=255)                                                                               gTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTCCGCCCTGGAGCCGGGTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGGTTGAt                                                                     >  8:266763/1‑149 (MQ=255)                                                                                tCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGa                                                                      >  3:172081/1‑147 (MQ=255)                                                                                tCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATa                                                                    <  7:35962/149‑1 (MQ=255)                                                                                  gTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTc                                                                                                                        <  8:221462/96‑1 (MQ=255)                                                                                  gTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTc                                                                                                                        >  5:9592/1‑96 (MQ=255)                                                                                  gTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTc                                                                                                                        >  7:221462/1‑96 (MQ=255)                                                                                  gTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTc                                                                                                                        <  6:9592/96‑1 (MQ=255)                                                                                   ttGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTc                                                                 >  3:261516/1‑149 (MQ=255)                                                                                      aTGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTCAAGCGGGTCGTGGTGGGCTTGATAGTCGa                                                               <  1:11802/149‑1 (MQ=255)                                                                                      aTGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAg                                                              <  5:150972/149‑1 (MQ=255)                                                                                        gtgCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGc                                                                                        >  3:26844/1‑121 (MQ=255)                                                                                        gtgCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGc                                                                                        <  4:26844/121‑1 (MQ=255)                                                                                           cTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTg                                                          <  1:259404/148‑1 (MQ=255)                                                                                           cTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTg                                                          <  6:85009/148‑1 (MQ=255)                                                                                             tGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCg                                                                                  <  8:132998/122‑1 (MQ=255)                                                                                             tGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCg                                                                                  >  7:132998/1‑122 (MQ=255)                                                                                             tGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGcc                                                       >  4:118716/1‑149 (MQ=255)                                                                                             tGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGcc                                                       >  8:210356/1‑149 (MQ=255)                                                                                               aaGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTg                                                     <  6:90979/149‑1 (MQ=255)                                                                                                  cGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGc                                                  <  2:71054/149‑1 (MQ=255)                                                                                                    gTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGt                                                >  7:92284/1‑149 (MQ=255)                                                                                                     tCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGGACAGGTGAGTACGCCCTGGAGCCGGTTTTCGATCAAGCTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTAGAGTTTGGCCTGGGCGtc                                               >  6:107489/1‑148 (MQ=255)                                                                                                       gAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCt                                             <  7:339904/149‑1 (MQ=255)                                                                                                         ggTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGGGTGCtg                                            >  2:342049/1‑147 (MQ=255)                                                                                                           tCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGa                                                                      <  5:292058/120‑1 (MQ=255)                                                                                                           tCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGa                                                                      >  6:292058/1‑120 (MQ=255)                                                                                                              aGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCt                                      <  7:210356/149‑1 (MQ=255)                                                                                                                    aGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGAt                                <  2:312677/149‑1 (MQ=255)                                                                                                                      cACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGTGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGt                              >  8:49854/1‑149 (MQ=255)                                                                                                                        cTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGTGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCt                            <  7:49854/149‑1 (MQ=255)                                                                                                                               cTGGTGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCaaaaa                     >  2:156893/1‑149 (MQ=255)                                                                                                                                 ggCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAAcc                   >  5:254084/1‑149 (MQ=255)                                                                                                                                      ttGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCAc              <  4:172081/149‑1 (MQ=255)                                                                                                                                        actcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTTCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACg             >  5:260039/4‑148 (MQ=255)                                                                                                                                         gACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGaa           <  4:261516/149‑1 (MQ=255)                                                                                                                                          tcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGc                                                                                        <  6:306830/70‑1 (MQ=255)                                                                                                                                          tcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGc                                                                                        >  5:306830/2‑71 (MQ=255)                                                                                                                                          tcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCaaaa                      <  5:68116/136‑1 (MQ=255)                                                                                                                                          tcacCTGTGCGCAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCaaaa                      >  6:68116/2‑137 (MQ=255)                                                                                                                                          acacCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGgag                                  <  4:26051/125‑1 (MQ=255)                                                                                                                                              cTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGga                                   >  8:33602/1‑120 (MQ=255)                                                                                                                                              cTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGga                                   <  7:33602/120‑1 (MQ=255)                                                                                                                                               tGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGGGGGt     >  4:141522/1‑149 (MQ=255)                                                                                                                                                  gcACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTGGGTGAt  <  8:92284/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCAACGTCGCGTACCTGCTCTTTCAGGCGCTGGGCGATGCCCTTGAGCAGC‑AGGTCGCCGACCGGGTGGCCAAGGCTGTCGTTGATGTGCTTGAAGCGGTCGAGGTCCAGGAACAGCACTGCGCCCTGGCGGTTGGACACCTGCGCACAGGTGAGTACGCCCTGGAGCCGGTTTTCGAACAAGGTGCGATTGGGCAGGCCGGTC‑AGCGGGTCGTGGTGGGCTTGATAGTCGAGTTTGGCCTGGGCGTGCTTGATGCTGGAGATGTCTGCAAAAACCGCCACGAAGTGGGTGAT  >  NC_002947/468318‑468610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑