Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,338,065 C→T 5.9% intergenic (+72/‑212) PP_4696 → / → PP_mr53 nitrogen regulation protein NR(I)/CrcZ ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,338,0650CT 5.9% 248.4 / 4.1 84intergenic (+72/‑212)PP_4696/PP_mr53nitrogen regulation protein NR(I)/CrcZ ncRNA
Reads supporting (aligned to +/- strand):  ref base C (40/39);  new base T (2/3);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

AGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGCCAAAAACTGCAG  >  NC_002947/5337919‑5338211
                                                                                                                                                  |                                                                                                                                                  
aGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCt                                                                                                                                                  <  8:315442/149‑1 (MQ=255)
   cGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTca                                                                                                                                                >  7:98089/1‑148 (MQ=255)
    gCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCTcacc                                                                                                                                              <  2:308496/149‑2 (MQ=255)
          gCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCt                                                                                                                                        >  4:314470/1‑149 (MQ=255)
             tgtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGaa                                                                                                                                     >  2:7752/1‑149 (MQ=255)
              gtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAAc                                                                                                                                    >  7:7943/1‑149 (MQ=255)
               tgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAAc                                                                                                                                    <  6:209983/148‑1 (MQ=255)
               tgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGCCTGTGAGGCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGacgccgcata                                                                                                                                   <  6:86973/149‑7 (MQ=25)
                  gAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                                <  4:233410/149‑1 (MQ=255)
                      gccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCa                                                                                                                              <  7:280423/147‑1 (MQ=255)
                      gccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCa                                                                                                                              >  8:280423/1‑147 (MQ=255)
                         gccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgac                                                                                                                          >  8:332644/1‑148 (MQ=255)
                          ccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgacg                                                                                                                         >  2:68539/1‑148 (MQ=255)
                              cgcCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCTAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAacac                                                                                                                    <  8:98089/149‑1 (MQ=255)
                                ccTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACAccc                                                                                                                  <  2:128006/149‑1 (MQ=255)
                                ccTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACAccc                                                                                                                  <  6:356987/149‑1 (MQ=255)
                                    ggCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAg                                                                                                               <  2:117271/148‑1 (MQ=255)
                                           cACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacga                                                                                                                           >  8:105187/1‑129 (MQ=255)
                                           cACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacga                                                                                                                           <  7:105187/129‑1 (MQ=255)
                                             cGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacctc                                                                                                     <  1:333780/149‑1 (MQ=255)
                                                  cAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACacctcacctc                                                                                                <  8:109355/149‑1 (MQ=255)
                                                   aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGCCGAACACCCCAGGTAACACCTCCACTcc                                                                                               >  7:247270/1‑149 (MQ=255)
                                                      agCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAg                                                                                                               <  7:347208/130‑1 (MQ=255)
                                                      agCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAg                                                                                                               >  8:347208/1‑130 (MQ=255)
                                                       gCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGt                                                                                            <  8:27316/148‑1 (MQ=255)
                                                        cAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGt                                                                                                             >  2:352103/1‑130 (MQ=255)
                                                        cAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGt                                                                                                             <  1:352103/130‑1 (MQ=255)
                                                           cGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTaaaaa                                                                                       <  3:314470/149‑1 (MQ=255)
                                                             cTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATc                                                                                     <  7:332644/149‑1 (MQ=255)
                                                              tACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTACCGTAAAAATCt                                                                                    >  2:286459/1‑149 (MQ=255)
                                                              tACCAGCGAATAACCCACCTACGCACAGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGGAAAAATCt                                                                                    >  3:224467/1‑149 (MQ=255)
                                                                ccAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                                    <  2:57902/147‑1 (MQ=255)
                                                                ccAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                                    <  2:217778/147‑1 (MQ=255)
                                                                ccAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGt                                                                                  <  1:186342/149‑1 (MQ=255)
                                                                  aGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACCTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGtt                                                                                 >  4:194953/1‑148 (MQ=255)
                                                                   gCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                               >  4:294248/1‑149 (MQ=255)
                                                                   gCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                               >  8:53249/1‑149 (MQ=255)
                                                                    cGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTTACACCCAGCGCCCCCCGCGCGGCGCTCGGCCACACAAGGCCAGAACGTGGCAAGACGAACACCCCCGCTAACACCAAACCTCCCCTAAAAATCTGTTaac                                                                              >  6:242611/1‑147 (MQ=255)
                                                                     gAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAccc                                                                             <  8:7943/149‑1 (MQ=255)
                                                                        tAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAg                                                                          <  1:68539/149‑1 (MQ=255)
                                                                        tAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAg                                                                          <  4:83079/149‑1 (MQ=255)
                                                                          aCCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATc                                                                                     >  4:35032/1‑136 (MQ=255)
                                                                          aCCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATc                                                                                     <  3:35032/136‑1 (MQ=255)
                                                                           cccACCTACGCACCGCACGCCGCAGCCTTTGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCCGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCtt                                                                       >  1:214300/1‑149 (MQ=255)
                                                                           cacACCTACGCACCGCACGCCGCAGCCTAAGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCtt                                                                       >  8:20845/3‑149 (MQ=255)
                                                                                    gcaccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGt                                                               >  3:340967/1‑148 (MQ=255)
                                                                                           acGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcc                                                       <  2:113933/149‑1 (MQ=255)
                                                                                               cgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTcccc                                                     >  5:107185/1‑147 (MQ=255)
                                                                                                 cAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAgg                                                 >  2:267008/1‑149 (MQ=255)
                                                                                                  aGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGc                                                <  1:286459/149‑1 (MQ=255)
                                                                                                     gatccgtgcgTAGGTGACACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTCTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAACCTTTCCGCCGTAACAGTCCCCGAGGCGTa                                             <  8:237346/142‑1 (MQ=38)
                                                                                                        tGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACg                                           <  6:371547/148‑1 (MQ=255)
                                                                                                           tgcgTAGGTGAGACCGAGCGCCTCCCGCGCGGCGCTCGGCCTAACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACGTCCCGTAAAAATCAGTTACCCAAGCTTTCCGCCGGACCAGTGCCCGAGGCGTAcagaac                                       >  7:237346/2‑144 (MQ=255)
                                                                                                            gcgTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAAc                                      <  1:7752/149‑1 (MQ=255)
                                                                                                               tCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGa                                    >  2:38500/1‑148 (MQ=255)
                                                                                                               tCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTAGGCCTCACAGGCGCTGAACGTGTCACGCCGAGCACCCCAGGTAACCCCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGTCGTACGGTAACgt                                    >  6:71659/1‑147 (MQ=39)
                                                                                                                  gTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTACCACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTACCAGTCCCCGAGGCGTACGGTAACGAAAtt                                >  3:299823/1‑149 (MQ=255)
                                                                                                                   tGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTg                               <  3:294248/149‑1 (MQ=255)
                                                                                                                     agaCCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGc                              <  7:20845/148‑1 (MQ=255)
                                                                                                                     aaagagAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCg                             <  8:37528/144‑1 (MQ=255)
                                                                                                                       gccGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                            >  5:105041/2‑148 (MQ=255)
                                                                                                                       gccGAGCGCCGCGCGGGCGGCGCTCGGTCTCACCGGCCCTGAACGTGTCACGACCAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCCAGGCGTACGGAAACGAAATTGCGa                            >  5:54086/2‑148 (MQ=38)
                                                                                                                       aCCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGATCACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTCCCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                            >  3:354751/1‑148 (MQ=255)
                                                                                                                       aCCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTCCCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                            >  3:360487/1‑148 (MQ=255)
                                                                                                                       aCCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                            >  1:153943/1‑148 (MQ=255)
                                                                                                                       aCCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                            >  4:232501/1‑148 (MQ=255)
                                                                                                                          gAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtt                        <  6:107185/149‑1 (MQ=255)
                                                                                                                             cgccgcCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTaa                                       <  7:284720/131‑1 (MQ=255)
                                                                                                                             cgccgcCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTaa                                       >  8:284720/1‑131 (MQ=255)
                                                                                                                               ccgcccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCCTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGaa                   <  1:267008/149‑1 (MQ=255)
                                                                                                                                cgcccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTACCCGAGGCGTACGGTAACGAAATTGAGACATTTTGAAc                  >  3:147844/1‑149 (MQ=255)
                                                                                                                                 gcccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAAcc                 <  7:53249/149‑1 (MQ=255)
                                                                                                                                  cccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCt                >  1:254207/1‑149 (MQ=255)
                                                                                                                                  cccgcGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCACCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCt                >  3:270797/1‑149 (MQ=255)
                                                                                                                                    cgcgcgGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGc              <  4:340967/149‑1 (MQ=255)
                                                                                                                                     gcgcgGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGc              >  4:299507/1‑148 (MQ=255)
                                                                                                                                         ggcgCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGCCATTTTGAACCTGCCaaa          >  8:277872/1‑148 (MQ=255)
                                                                                                                                         ggcgCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGCCaaa          >  4:116583/1‑148 (MQ=255)
                                                                                                                                         ggcgCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGCCaaa          >  7:84182/1‑148 (MQ=255)
                                                                                                                                          gcgcTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGCCtaaaa        <  2:153943/149‑1 (MQ=255)
                                                                                                                                            gcTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACGCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTACCAGTCCCCGAGGCGTACTGTAACGAAATTGCGACATTTTGAACCTGCCAAAAACt      <  4:224467/149‑1 (MQ=255)
                                                                                                                                                ggCCTCACAGGCGCTGACCGTGTCACGAAGAACACCCCATGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGCCAAAAACTGc    <  4:354751/147‑1 (MQ=255)
                                                                                                                                                ggCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGCCAAAAACTGc    <  4:360487/147‑1 (MQ=255)
                                                                                                                                                 gCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGAAATTTTGAACCTGCCAAAAACTGCac  >  3:7815/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCGCGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGCCAAAAACTGCAG  >  NC_002947/5337919‑5338211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑