Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,344,484 C→G 10.5% intergenic (+59/‑119) pgi‑II → / → acsA‑II glucose 6‑phosphate isomerase/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,344,4840CG10.5% 92.0 / 2.4 36intergenic (+59/‑119)pgi‑II/acsA‑IIglucose 6‑phosphate isomerase/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (19/13);  new base G (2/2);  total (21/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

CAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGC  >  NC_002947/5344336‑5344631
                                                                                                                                                        |                                                                                                                                                   
cAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTAGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTc                                                                                                                                                     >  3:319384/1‑149 (MQ=255)
  gcgcTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCg                                                                                                                                                    >  5:70164/1‑148 (MQ=255)
   cgcTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTa                                                                                                                                                  <  4:174892/149‑1 (MQ=255)
   cgcTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTa                                                                                                                                                  <  5:303855/149‑1 (MQ=255)
     cTTCGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAg                                                                                                                                                 >  6:72178/1‑148 (MQ=255)
        ggTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGa                                                                                                                                               <  2:275008/147‑1 (MQ=255)
              tAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGttt                                                                                                                                       <  5:72178/149‑1 (MQ=255)
              gaGCCTGGACGACAGCGCCGAAGACTGTTAGACCAAAGTTCTGATCAACTCCTTCCGCGGCCGTCAGCGCGGTTGATCCGAGCTTGTCCCGGCCTCTTCGCGGTTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGttt                                                                                                                                       <  8:285785/148‑1 (MQ=255)
                gCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACGCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAc                                                                                                                                     >  5:321738/1‑149 (MQ=255)
                 ccTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAcc                                                                                                                                    <  7:61892/149‑1 (MQ=255)
                  cTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCTC‑‑CTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTAccc                                                                                                                                   <  1:140307/149‑1 (MQ=21)
                         aCAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAccc                                                                                                                                   >  4:279839/1‑142 (MQ=255)
                         aCAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAccc                                                                                                                                   <  3:279839/142‑1 (MQ=255)
                             cgcCGAAGACGGTTCCACCCAAGGCCTTATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGTTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAggcc                                                                                                                        <  7:177302/149‑1 (MQ=255)
                                cGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCggg                                                                                                                                          <  7:267174/128‑1 (MQ=255)
                                cGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCggg                                                                                                                                          >  8:267174/1‑128 (MQ=255)
                                             aCCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CGACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAacac                                                                                                        >  6:110789/1‑149 (MQ=255)
                                                       tGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTAGCGGGTAAAACCG‑‑C‑‑CTACCAGGCAACCCGTTACCTCGTAGGAGCCGGTTTACCCGCGAAGAGGCCGGGCCAGGTTGGACACCGCTACCAGc                                                                                              >  1:299590/1‑149 (MQ=38)
                                                         aTCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGt                                                                                                                                         >  4:287801/1‑104 (MQ=255)
                                                         aTCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGt                                                                                                                                         <  3:287801/104‑1 (MQ=255)
                                                               tactTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTAGCGGGTTAACCCG‑‑C‑‑CGACCCGGCAACGCGGTACCGCGTAGGGGCGGGGTTAACCGCGCAGAGGCCGGGCCCGGGTGCACCCCGCGCCCCGCGACACAGt                                                                                      >  8:79279/1‑149 (MQ=255)
                                                                  ttCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCt                                                                                    >  8:87983/1‑148 (MQ=255)
                                                                     cgcgGCCGTCCCCGCGGTTTATCCGAGCTTGTGCCGGCCTCTTGGCGGGTAACCCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCTTCGGAGCGGGTTAACCCGCTAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTAt                                                                                 <  6:70164/148‑1 (MQ=255)
                                                                          ccGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCTTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATa                                                                           <  7:87983/149‑1 (MQ=255)
                                                                                 cggggTTGATCCGCGCTTGTGCCGGCCTCTTCGCGGGTAACCCCG‑‑C‑‑CTACCAGGCAACGCTTTACCTGGTTGGTGGGGGTTTACCCGCGATTTGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTg                                                                    <  4:319384/146‑1 (MQ=255)
                                                                                                        ggCCTCTCGGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTATTAGCGGGTTTACCCGCTAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAagag                                             <  5:110789/149‑1 (MQ=255)
                                                                                                            tctTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAg                                          >  2:10457/1‑148 (MQ=255)
                                                                                                            tctTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCACTTACAAGAGCAg                                          >  6:189191/1‑148 (MQ=255)
                                                                                                                   gggTAAACCCGCTC‑‑CTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCAccc                                  >  3:51689/1‑149 (MQ=21)
                                                                                                                     gTAAACCCG‑‑CTACTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGACGTTGCAGTCCATCGCACCAAACAagag                                             >  6:26725/1‑136 (MQ=18)
                                                                                                                         aCCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCCCCCGCCATg                            >  2:85683/1‑149 (MQ=255)
                                                                                                                           ccG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGtt                          >  8:354976/1‑149 (MQ=255)
                                                                                                                                      ccAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCCCTCCAAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGCCCAGCCGCCAAGTACGATATc                   >  6:6822/1‑149 (MQ=39)
                                                                                                                                            aaCGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCaaa             >  4:256802/1‑149 (MQ=255)
                                                                                                                                                  ttACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACATGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATAcccc       >  5:318897/1‑149 (MQ=255)
                                                                                                                                                       tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCGCAGGTTGACCACCGCTCCCAGCTACACTGTCTTATCGAATAGGCGTTGCAGTCCGTCGCCCCGAGCAAGAGCAGGACCACACGCCATGTTCGATATCCGCAAATACCCCCCGGc  >  8:281022/1‑149 (MQ=38)
                                                                                                                                                       tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGc  >  5:48586/1‑149 (MQ=255)
                                                                                                                                                        cGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGa                                        >  5:115072/1‑110 (MQ=255)
                                                                                                                                                        cGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGa                                        <  6:115072/110‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                   
CAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑C‑‑CTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGC  >  NC_002947/5344336‑5344631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑