Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,345,552	T→G	6.2%	L317R (CTG→CGG)	acsA‑II →	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,345,552	0	T	G	6.2%	189.2 / 2.6	64	L317R (CTG→CGG)	acsA‑II	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (25/35);  new base G (2/2);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

AAGGGGTGCTGCATACCACCGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGCGTGAGGGCTCCGC  >  NC_002947/5345405‑5345699                                                                                                                                                    |                                                                                                                                                        aaGGGGTGCTGCATACCACCGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTg                                                                                                                                                        <  6:337459/149‑1 (MQ=255)      gTGCTGCATACCACCGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCaa                                                                                                                                                   <  5:103549/149‑1 (MQ=255)         ctgcATACCACCGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATg                                                                                                                                                 <  7:304720/148‑1 (MQ=255)         ctgcATACCACCGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCAGCGGGCCataga                                                                                                                                                <  8:206121/149‑5 (MQ=255)          tgcATACCACCGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGgc                                                                                                                                               >  3:275642/1‑149 (MQ=255)                   ccGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCg                                                                                                                                     <  1:352868/149‑1 (MQ=255)                    cggcggCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGc                                                                                                                                    <  3:72593/149‑1 (MQ=255)                    cggcggCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGc                                                                                                                                    >  2:250569/1‑149 (MQ=255)                     ggcggcTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCt                                                                                                                                   <  4:275642/149‑1 (MQ=255)                     ggcggcTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCt                                                                                                                                   <  2:42525/149‑1 (MQ=255)                     ggcggcTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCt                                                                                                                                   <  2:254506/149‑1 (MQ=255)                                gctgcAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAg                                                                                                                        >  5:354500/1‑149 (MQ=255)                                 ctgcAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGaa                                                                                                                         <  3:33914/147‑1 (MQ=255)                                 ctgcAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAg                                                                                                                        <  4:370357/148‑1 (MQ=255)                                 ctgcAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCGAGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAg                                                                                                                        <  6:358348/148‑1 (MQ=255)                                            gATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAAc                                                                                                             >  3:99812/1‑148 (MQ=255)                                                 ccTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTAcccc                                                                                                       <  2:248180/149‑1 (MQ=255)                                                  cTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTAcccc                                                                                                       >  5:214361/1‑148 (MQ=255)                                                   ttCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCg                                                                                                      >  5:331820/1‑148 (MQ=255)                                                   ttCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGa                                                                                                     >  6:13524/1‑149 (MQ=255)                                                   ttCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATATTCGAAGGCGTGCCCAACTACCCCGa                                                                                                     >  5:303031/1‑149 (MQ=255)                                                   ttCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCCGATGTTCGAAGGCGTGCCCAACTACCCCGa                                                                                                     >  5:69400/1‑149 (MQ=255)                                                     gaaGGTGGTGTTCGTTTATCGTGACGGCGAGGTGTTCTGGTGCAGCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GGAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGa                                                                                                     <  5:70496/146‑1 (MQ=255)                                                     cAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCg                                                                                                                                     >  2:18593/1‑115 (MQ=255)                                                     cAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGa                                                                                                     <  4:159775/147‑1 (MQ=255)                                                      aaGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑Ac                                                                                               <  5:122701/149‑1 (MQ=255)                                                      aaGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑Ac                                                                                               <  7:63827/149‑1 (MQ=255)                                                         gtggtgTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTc                                                                                            <  5:358153/149‑1 (MQ=255)                                                            gtgtTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTcgcg                                                                                         >  4:140213/1‑149 (MQ=255)                                                              gtTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCt                                                                                       >  7:143262/1‑149 (MQ=255)                                                                  gATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCt                                                                                    <  6:344358/148‑1 (MQ=255)                                                                    ttATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCaa                                                                                                              <  5:156417/123‑1 (MQ=255)                                                                    ttATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCaa                                                                                                              >  6:156417/1‑123 (MQ=255)                                                                    ttATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTgg                                                                                  >  1:55426/1‑148 (MQ=255)                                                                         gTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAAAGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTACTGGCAggtg                                                                            >  2:307468/1‑149 (MQ=255)                                                                          tGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAggtgg                                                                           <  5:13524/149‑1 (MQ=255)                                                                          tGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAggtgg                                                                           >  2:241046/1‑149 (MQ=255)                                                                          tGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCAAATGTC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAggtgg                                                                           >  7:238051/1‑149 (MQ=255)                                                                            aCGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAggtggt                                                                          <  1:250569/148‑1 (MQ=255)                                                                             cGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGtggtgg                                                                        <  6:217169/149‑1 (MQ=255)                                                                              ggCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGa                                                                       >  8:182756/1‑149 (MQ=255)                                                                                cGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACa                                                                     <  1:241046/149‑1 (MQ=255)                                                                                   ggagtTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGc                                                                  <  3:200397/146‑1 (MQ=255)                                                                                     tgtTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCAc                                                                <  4:99812/149‑1 (MQ=255)                                                                                         cTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAgg                                                            >  2:164273/1‑149 (MQ=255)                                                                                            gTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGt                                                           <  6:214361/147‑1 (MQ=255)                                                                                               cACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGGCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACa                                                      >  1:366389/1‑149 (MQ=255)                                                                                                     cgATGTCGGCTGGGGGACCGGCCACATCTACATCCTCTAATGCCCGCTTGCCAATTGCTGGAATCTCGCTGATTTTCGAATGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACAtcttc                                                 <  1:308002/149‑1 (MQ=255)                                                                                                                   tGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGAcc                                  >  6:328925/1‑149 (MQ=255)                                                                                                                    gACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCg                                 >  3:331781/1‑149 (MQ=255)                                                                                                                            acaGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCTTGCCCAACTACCCAGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCATgcgc                         <  3:73438/149‑1 (MQ=255)                                                                                                                             caGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGATCTCGCCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTgc                           <  1:307468/149‑1 (MQ=38)                                                                                                                                cTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGCCCGCCCTGCGCGCa                      >  8:141192/1‑148 (MQ=255)                                                                                                                                cTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCAc                     <  6:331820/149‑1 (MQ=255)                                                                                                                                cTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCAc                     <  6:105363/149‑1 (MQ=255)                                                                                                                                  aCATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGTACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACt                    >  4:284786/1‑148 (MQ=255)                                                                                                                                      cGTCTATGGACAGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGc               >  4:184333/1‑149 (MQ=255)                                                                                                                                       gccattGGCCAGCGGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTATCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATg                <  1:20264/142‑1 (MQ=255)                                                                                                                                       gccattGGCCAGCGGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTATCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATg                >  2:20264/6‑147 (MQ=255)                                                                                                                                       gTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGc               <  3:140213/148‑1 (MQ=255)                                                                                                                                        tCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGCGt             <  5:138294/149‑1 (MQ=255)                                                                                                                                         cattGGCCAGCGGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGCGt             >  8:73503/4‑148 (MQ=255)                                                                                                                                            tGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGCGTGa           <  7:182756/147‑1 (MQ=255)                                                                                                                                                   cTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGCGTGAGGGCTCcgc  <  8:143262/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                        AAGGGGTGCTGCATACCACCGGCGGCTACCTGCTGCAGGCAACGATGACCTTCAAGGTGGTGTTCGATTATCGTGACGGCGAGGTGTTCTGGTGCACCGCCGATGTCGGCTGGGTGACCGGCCACAGCTACATCGTCTATGGCCCGCTGGCCAATGGC‑GCAATCTCGCTGATGTTCGAAGGCGTGCCCAACTACCCCGAT‑‑‑ACCTCGCGCTTCTGGCAGGTGGTGGACAAGCACCAGGTGAACATCTTCTACACCGCCCCGACCGCCCTGCGCGCACTGATGCGTGAGGGCTCCGC  >  NC_002947/5345405‑5345699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑