Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,367,621	A→T	5.5%	K48M (AAG→ATG)	PP_4720 →	RNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,367,621	0	A	T	5.5%	224.3 / 2.7	73	K48M (AAG→ATG)	PP_4720	RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (29/40);  new base T (2/2);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

ATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGT  >  NC_002947/5367476‑5367764                                                                                                                                                  |                                                                                                                                                aTTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCTTGATCAGCTCATGGt                                                                                                                                              >  5:131891/1‑149 (MQ=255)     tGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCTTGATCAGCTCATGGTCga                                                                                                                                           <  6:199050/148‑1 (MQ=255)       ccGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCTTGATCAGCTCATGGTCgacca                                                                                                                                        <  6:131891/149‑4 (MQ=255)       ccGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGAt                                                                                                                                        <  6:257900/149‑1 (MQ=255)         gCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTc                                                                                                                                      <  3:222110/149‑1 (MQ=255)             aaTAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTc                                                                                                                                  >  5:140459/1‑149 (MQ=255)               tAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGCTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCgg                                                                                                                                <  1:121210/149‑1 (MQ=255)                aaCGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCgg                                                                                                                                >  6:331461/1‑148 (MQ=255)                 aCGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGaa                                                                                                                              <  6:90411/149‑1 (MQ=255)                        gaagaaGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgcg                                                                                                                       <  7:340925/149‑1 (MQ=255)                         aagaagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgcg                                                                                                                       >  8:104587/1‑148 (MQ=255)                             agaaaTCCAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACTGTTTGAATGAATGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCgaagaa                                                                                                                  <  7:107868/146‑1 (MQ=255)                             agCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCgaag                                                                                                                    >  2:326836/1‑147 (MQ=255)                              gCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAAc                                                                                                                 >  2:74669/1‑149 (MQ=255)                                     aaGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGcc                                                                                                            >  8:70383/1‑147 (MQ=255)                                       gTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCgaga                                                                                                        <  3:305339/149‑1 (MQ=255)                                       gTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCgaga                                                                                                        >  6:105762/1‑149 (MQ=255)                                       gTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGGCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCgaga                                                                                                        <  2:146487/149‑1 (MQ=255)                                          cATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCa                                                                                                     >  3:290368/1‑149 (MQ=255)                                           aTTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCAt                                                                                                    <  1:326836/149‑1 (MQ=255)                                           aTTGGTCATGACCTGAAGCCGGACCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCAt                                                                                                    >  6:307992/1‑149 (MQ=255)                                            ttcgTCATGAGCTTAAGCCTGTCCTGATCGTTGCTGGCAACGTTTTTAATGAAGGCGTTATCGCCGAACTGGAGCGCTATCTGGTCGACCATGAGCTGATCAATGTCGAGATTCGCTCGGAAGACCGCGTAGAACGCGCCGAGACCATc                                                                                                   <  5:156261/146‑1 (MQ=255)                                            ttGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATc                                                                                                   >  2:135248/1‑149 (MQ=255)                                                  aTGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGa                                                                                              <  7:304510/148‑1 (MQ=255)                                                  aTGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGa                                                                                              <  1:135248/148‑1 (MQ=255)                                                  aTGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAg                                                                                             <  1:74669/149‑1 (MQ=255)                                                      ccTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCt                                                                                           >  5:64251/1‑147 (MQ=255)                                                          aaGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGc                                                                                       >  5:237937/1‑147 (MQ=255)                                                               ggTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAggccg                                                                                <  1:318403/149‑1 (MQ=255)                                                                gTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAggccgg                                                                               >  7:147252/1‑149 (MQ=255)                                                                  ccTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAggccggcc                                                                             <  4:304800/149‑1 (MQ=255)                                                                      aTCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGc                                                                         >  3:78660/1‑149 (MQ=255)                                                                       tCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGcc                                                                        <  6:277498/149‑1 (MQ=255)                                                                       tCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGcc                                                                        <  6:85638/149‑1 (MQ=255)                                                                         gTTGCTGGCAACGGTTTGAATGAAGGCGTGAGCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGa                                                                      <  7:168863/149‑1 (MQ=255)                                                                          ttGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAg                                                                     >  2:133989/1‑149 (MQ=255)                                                                          taccTGGCAACGGTTTGAAAGAAGGCGTCATCGCCGAACTGGACCGCGCTCTGGTCGACCAAGAGCTCATCTAGGTCGAGATTCGCACGGAAGACCGCGAAGAAAGCGCCGAGACCATCGCCGAGCTCTCCAAGCCCGGCAGTGCCGAg                                                                     >  8:286327/4‑149 (MQ=255)                                                                             cTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTg                                                                  <  5:307992/149‑1 (MQ=255)                                                                             cTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTg                                                                  >  4:154360/1‑149 (MQ=255)                                                                             cTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTg                                                                  >  2:344124/1‑149 (MQ=255)                                                                                gCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTgg                                                                 <  6:237937/147‑1 (MQ=255)                                                                                gCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTgg                                                                 <  5:66915/147‑1 (MQ=255)                                                                                gCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTgg                                                                 <  1:341179/147‑1 (MQ=255)                                                                                    cGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGa                                                           <  5:331461/149‑1 (MQ=255)                                                                                    cGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGa                                                           <  6:140459/149‑1 (MQ=255)                                                                                                  gCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGa                                               >  5:147797/1‑147 (MQ=255)                                                                                                       aTCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGccc                                         <  7:70383/148‑1 (MQ=255)                                                                                                         cGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGc                                                                    >  7:203898/1‑119 (MQ=255)                                                                                                         cGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGc                                                                    <  8:203898/119‑1 (MQ=255)                                                                                                          gCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGa                                      <  4:56199/148‑1 (MQ=255)                                                                                                          gCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGAt                                     >  1:167407/1‑149 (MQ=255)                                                                                                          gCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGAt                                     >  7:349729/1‑149 (MQ=255)                                                                                                           ccGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGt                                                                <  4:138890/121‑1 (MQ=255)                                                                                                           ccGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGt                                                                >  3:138890/1‑121 (MQ=255)                                                                                                            cGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                               <  3:348667/121‑1 (MQ=255)                                                                                                            cGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                               >  4:348667/1‑121 (MQ=255)                                                                                                              aaCTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTAc                                 <  7:104587/149‑1 (MQ=255)                                                                                                              aaCTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTAc                                 <  4:290368/149‑1 (MQ=255)                                                                                                                 tGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCg                               >  4:119145/1‑148 (MQ=255)                                                                                                                           tctGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCca                     >  1:147509/1‑148 (MQ=255)                                                                                                                             tGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCca                     <  2:147509/146‑1 (MQ=255)                                                                                                                                tCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCg               <  6:147797/149‑1 (MQ=255)                                                                                                                                  gACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGaa             >  4:47850/1‑149 (MQ=255)                                                                                                                                    ccATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAAc            >  1:220488/1‑148 (MQ=255)                                                                                                                                     cATGAGCTGATCAAGGTCGAGATGCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACaa          <  8:147252/149‑1 (MQ=255)                                                                                                                                      aTGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAag         <  2:167407/149‑1 (MQ=255)                                                                                                                                       tGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAagc        <  4:78660/149‑1 (MQ=255)                                                                                                                                        gatcagctCATGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAag         >  3:286056/8‑147 (MQ=255)                                                                                                                                         aGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAagcag      <  5:105762/149‑1 (MQ=255)                                                                                                                                             gATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAAccc                      <  2:375992/129‑1 (MQ=255)                                                                                                                                             gATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAAccc                      >  1:375992/1‑129 (MQ=255)                                                                                                                                              aTCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGt  <  3:154360/148‑1 (MQ=255)                                                                                                                                              aTCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGt  <  1:344124/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                ATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGCTGT  >  NC_002947/5367476‑5367764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑