Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,382,270 A→T 8.2% I125F (ATC→TTC)  smpB → SsrA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,382,2700AT 8.2% 277.8 / 10.6 97I125F (ATC→TTC) smpBSsrA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (42/46);  new base T (4/4);  total (47/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGGCAA  >  NC_002947/5382127‑5382416
                                                                                                                                               |                                                                                                                                                  
aCGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCa                                                                                                                                               <  2:48582/149‑1 (MQ=255)
  gTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACt                                                                                                                                             <  6:63289/149‑1 (MQ=255)
  gTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACt                                                                                                                                             >  7:43049/1‑149 (MQ=255)
  gTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACAACTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACt                                                                                                                                             <  7:354573/149‑1 (MQ=255)
   tCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTg                                                                                                                                            <  2:699/149‑1 (MQ=255)
   tCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTg                                                                                                                                            >  7:289085/1‑149 (MQ=255)
    cATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgg                                                                                                                                           >  2:139597/1‑149 (MQ=255)
     aTCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgg                                                                                                                                           <  1:139597/148‑1 (MQ=255)
        gCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCACttgatc                                                                                                                                        <  3:329822/148‑6 (MQ=255)
         ccGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCACttgatcag                                                                                                                                      <  4:364464/149‑8 (MQ=255)
         ccGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaag                                                                                                                                      <  8:285521/149‑1 (MQ=255)
           gACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggcaaggg                                                                                                                                    >  2:74634/1‑149 (MQ=255)
                tACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGggcaagggcaag                                                                                                                                >  6:220252/1‑148 (MQ=255)
                  cacGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag                                                                                                                             <  1:5358/149‑1 (MQ=255)
                  cacGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag                                                                                                                             <  8:46394/149‑1 (MQ=255)
                  cacGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaa                                                                                                                              <  4:57312/148‑1 (MQ=255)
                  cacGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaa                                                                                                                              <  5:220252/148‑1 (MQ=255)
                  cacGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACATGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag                                                                                                                             >  8:348922/1‑149 (MQ=255)
                        cccGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTc                                                                                                                       <  6:260573/149‑1 (MQ=255)
                            cAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACa                                                                                                                   <  1:74634/149‑1 (MQ=255)
                             aaGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACaa                                                                                                                  <  8:255543/149‑1 (MQ=255)
                               gctgctgctGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAgc                                                                                                                >  6:249474/1‑149 (MQ=255)
                                   ctgctgAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGaa                                                                                                                          <  6:236915/135‑1 (MQ=255)
                                   ctgctgAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGa                                                                                                            <  7:348922/149‑1 (MQ=255)
                                        gAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCACGCACCTGATCAAGTGCGAAGTCGCGCTGGGCAGGGGGAGGAGGGAGTGCGGGAGGCGCGaggcgg                                                                                                       >  4:267525/1‑144 (MQ=255)
                                          aCAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCAt                                                                                                      <  1:322131/148‑1 (MQ=255)
                                           cAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGTCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGTTCAAGAAGGAATTCGACAAGCGCGACACCATgc                                                                                                    <  6:201521/149‑1 (MQ=255)
                                           cAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATgc                                                                                                    >  6:368868/1‑149 (MQ=255)
                                           cAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATgc                                                                                                    <  1:136090/149‑1 (MQ=255)
                                              gcgcGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGggcaagggcaag                                                                                                                                <  8:61623/118‑1 (MQ=255)
                                              gcgcGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGggcaagggcaag                                                                                                                                >  7:61623/1‑118 (MQ=255)
                                                 cgAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGac                                                                                                           >  3:367339/1‑136 (MQ=255)
                                                 cgAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGac                                                                                                           <  4:367339/136‑1 (MQ=255)
                                                     cTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGa                                                                                          >  2:274350/1‑149 (MQ=255)
                                                        gAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTc                                                                                       >  6:198539/1‑149 (MQ=255)
                                                        gAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTc                                                                                       >  7:75038/1‑149 (MQ=255)
                                                           cgcgTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa                                                                                    <  2:343887/149‑1 (MQ=255)
                                                            gcgTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAAc                                                                                   <  5:198539/149‑1 (MQ=255)
                                                              gTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACcg                                                                                 >  6:62611/1‑149 (MQ=255)
                                                              gTGGAAGCGGACGTCGCGCAAAAAGGCTACACCTGTGTGGCTTTCGCGCTGTACTGGACCAACCACATGATCAACTCCGAAATCGCACTCGACAAGGGCAAGAACGAATTCGACAAGCGACACCCCCTGCGCGAACGCGATTCCAAcca                                                                                 >  8:180029/1‑148 (MQ=255)
                                                                 gAAGCGGCCGTGGCGCAAAAAGGCTACTCCTGTGTGTCTTTGGCGCGGTACTGCAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGCGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGa                                                                              <  8:123943/149‑1 (MQ=255)
                                                                 gAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACcgcg                                                                               <  8:43049/148‑1 (MQ=255)
                                                                   aGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa                                                                                    <  8:59386/141‑1 (MQ=255)
                                                                   aGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa                                                                                    >  7:59386/1‑141 (MQ=255)
                                                                   aGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGc                                                                            <  7:180029/149‑1 (MQ=255)
                                                                      ggCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGa                                                                                          <  8:135862/132‑1 (MQ=255)
                                                                      ggCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGa                                                                                          >  7:135862/1‑132 (MQ=255)
                                                                      ggCCGTGGCGCAAAAAGGCTACACCCGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTg                                                                          >  7:201210/1‑148 (MQ=255)
                                                                         cGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGc                                                                      >  3:299244/1‑149 (MQ=255)
                                                                             gcgcAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCggg                                                                   <  8:75038/148‑1 (MQ=255)
                                                                               gcAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCtg                                                                >  6:382416/1‑149 (MQ=255)
                                                                                cAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCtgt                                                               >  6:305958/1‑149 (MQ=255)
                                                                                 aaaaaGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCggg                                                                   >  8:300732/1‑144 (MQ=255)
                                                                                 aaaaaGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCggg                                                                   <  7:300732/144‑1 (MQ=255)
                                                                                  aaaaGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGc                                                             >  5:6037/1‑149 (MQ=255)
                                                                                  aaaaGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGc                                                             >  6:331715/1‑149 (MQ=255)
                                                                                   aaaGGCTTCACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATGCCAACCGCGAGCTGCAGCGGGCTGTGCg                                                            >  6:271031/1‑149 (MQ=255)
                                                                                           cacCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAgg                                                    <  4:268278/149‑1 (MQ=255)
                                                                                           cacCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAgg                                                    >  6:216929/1‑149 (MQ=255)
                                                                                              cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGc                                                             <  5:19371/137‑1 (MQ=255)
                                                                                              cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGc                                                             >  6:19371/1‑137 (MQ=255)
                                                                                              cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGc                                                  >  4:264601/1‑148 (MQ=255)
                                                                                              cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGc                                                  >  3:267689/1‑148 (MQ=255)
                                                                                                 tgtgGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAg                                               >  4:267074/1‑148 (MQ=255)
                                                                                                    ggCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAgg                                                    <  1:274539/140‑1 (MQ=255)
                                                                                                    ggCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAgg                                                    >  2:274539/1‑140 (MQ=255)
                                                                                                     gCTTTGGCGCTGTACTGGAGCAAGCACCTTATCATGTGCGAAATGGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAaga                                          <  5:249474/149‑1 (MQ=255)
                                                                                                     gCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAaga                                          <  5:368868/149‑1 (MQ=255)
                                                                                                      cTTTGGCGCTGTACTGGAGCAAGCACCTTATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAagag                                         <  8:289085/149‑1 (MQ=255)
                                                                                                       tttGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAagag                                         >  3:26176/1‑148 (MQ=255)
                                                                                                             gcTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAactac                                  >  7:118822/1‑149 (MQ=255)
                                                                                                               atgtcTGGAGCAAGCACGTGATCAAGTGCGAAATCGCACTTGGCAAGGGAAAGAAGTAATTCGACAAGAGCGACACCTTGCGCGAACGCGATTCCAACGGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTActc                                <  8:271486/145‑1 (MQ=255)
                                                                                                                    tGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGAAAAGCGCGACACCATGCGCGAACGCGATTCCAACAGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTActc                                >  7:271486/1‑144 (MQ=255)
                                                                                                                      gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAg                                                     <  8:346812/121‑1 (MQ=255)
                                                                                                                      gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAg                                                     >  7:346812/1‑121 (MQ=255)
                                                                                                                      gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAAcc                         >  2:44553/1‑149 (MQ=255)
                                                                                                                      gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAAcc                         >  2:335355/1‑149 (MQ=255)
                                                                                                                      gAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAAc                          <  4:299244/148‑1 (MQ=255)
                                                                                                                         caagcaCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGt                      >  3:182359/1‑149 (MQ=255)
                                                                                                                            gcaCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTcc                   <  8:148369/149‑1 (MQ=255)
                                                                                                                            gcaCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTcc                   <  3:267074/149‑1 (MQ=255)
                                                                                                                             caCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCg                  <  1:274350/149‑1 (MQ=255)
                                                                                                                             caCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCg                  <  7:32981/149‑1 (MQ=255)
                                                                                                                              aCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCgg                 >  4:5992/1‑149 (MQ=255)
                                                                                                                               ccTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGc                <  8:118822/149‑1 (MQ=255)
                                                                                                                                cttgcccaGTGCGATTTCGCACTTGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGcc               >  3:365786/8‑149 (MQ=255)
                                                                                                                                cTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCg                                                                     >  1:374583/1‑95 (MQ=255)
                                                                                                                                cTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCg                                                                     <  2:374583/95‑1 (MQ=255)
                                                                                                                                   aTCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCct              <  5:305958/147‑1 (MQ=255)
                                                                                                                                    cccaGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCtt           >  3:57893/4‑149 (MQ=255)
                                                                                                                                     ccaGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTc          >  4:368667/3‑149 (MQ=255)
                                                                                                                                       aGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGc                >  7:159566/1‑141 (MQ=255)
                                                                                                                                       aGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGc                <  8:159566/141‑1 (MQ=255)
                                                                                                                                        gTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTcgcg       <  4:267689/149‑1 (MQ=255)
                                                                                                                                         tGCGATTTCGCACTTGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg      <  4:365786/149‑1 (MQ=255)
                                                                                                                                          gCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTaa                                      >  6:40747/1‑116 (MQ=255)
                                                                                                                                            gAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGGc    >  6:96042/1‑148 (MQ=255)
                                                                                                                                              aaTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGGCaa  <  4:26176/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
ACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGGGCAA  >  NC_002947/5382127‑5382416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑